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pEGFP-C1-KSENB-Nup133

Plasmid

#87340

Purpose

To express human Nup133 in mammalian cells

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP133 (NUP133 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deletion of Nup133 AA1-7

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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Cyclin E-pBI-EGFP

Plasmid

#16654

Depositor

Bert Vogelstein

Article

Rajagopalan et al Nature. 2004 Mar 4. 428(6978):77-81.

Insert

Cyclin E (CCNE1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(+18)

Plasmid

#199162

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+18)

Use

Tags

MGHHHHHHGGA

Expression

Mutation

E16R, D29K, E42K, E44K, D86K, D112K, T115K, D127R…

Promoter

T7

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(+6)

Plasmid

#199160

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+6)

Use

Tags

MGHHHHHHGGA

Expression

Mutation

E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

Promoter

T7

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-hSYN-eGFP

Plasmid

#162512

Purpose

AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the human synapsin (hSYN) promoter

Depositor

Bart Nieuwenhuis

Article

Nieuwenhuis et al Gene Ther. 2020 Jun 23. pii: 10.1038/s41

Insert

eGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSYN

Available since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GGamma4-GFP2

Plasmid

#166776

Purpose

Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GGamma4-GFP2 (GNG4 Human, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Contains an N-terminal GFP2 and GSAG linker

Promoter

CMV

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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(R42A) Cyclin B1-GFP (62)

Plasmid

#61849

Purpose

mammalian expression of Cyclin B1 with R42A mutation in destruction box to make cyclin B1 non-destructable

Depositor

Jonathon Pines

Article

Clute et al Nat Cell Biol. 1999 Jun;1(2):82-7.

Insert

cyclin B1 (CCNB1 Human)

Use

Tags

GFP and Myc

Expression

Mammalian

Mutation

V5A R42A

Promoter

CMV

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-IRAlus-Lin28

Plasmid

#92347

Purpose

Plasmid for expression of GFP gene with a Lin28 gene derived IR Alu element containing 3'UTR.

Depositor

Gordon Carmichael, Ling-Ling Chen

Article

Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

Insert

Lin28 gene derived IR Alu element containing 3'UTR (LIN28A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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pET-45b-mEGFP

Plasmid

#185013

Purpose

Bacterial Expression of 6xHis Sirius-GFP Fusion Protein

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

Sirius-GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pTY137 psfGFP-bimax2

Plasmid

#202410

Purpose

Expresses sfGFP-bimax2 to inhibit importin-alpha-mediated nuclear import

Depositor

Tim Mitchison

Article

Yoo et al J Cell Biol. 2021 Jul 5;220(7). pii: 212

Insert

bimax2

Use

Tags

sfGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV CAGG eGFP

Plasmid

#107707

Purpose

express eGFP in variety of cell types

Depositor

Troy Margrie

Article

Velez-Fort et al Neuron. 2018 Apr 4;98(1):179-191.e6. doi

Insert

eGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAGG

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST47-CYFIP1.GFP

Plasmid

#109139

Purpose

GFP-tagged CYFIP1 expression construct

Depositor

Josef Kittler

Article

Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

Insert

CYFIP1 (CYFIP1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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RUNX1_pcDNA6.2/EmGFP-Bsd

Plasmid

#176974

Purpose

Mammalian expression vector encoding RUNX1 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

RUNX1 (RUNX1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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NLS-tdPCP-GFP

Plasmid

#183934

Purpose

PCP tandem dimer binding to PP7 RNA stem loops; labeled with GFP

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

tdPCP-GFP

Use

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV (TATA box removed)

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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Lact-C2-GFP-p416

Plasmid

#22853

Depositor

Sergio Grinstein

Article

Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

Insert

Lactadherin-C2

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
YIplac211-iGFP-TLG1

Plasmid

#105261

Purpose

Plasmid for pop-in/pop-out replacement of Tlg1 with iGFP-Tlg1.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Tlg1 (TLG1 Budding Yeast)

Use

Tags

iGFP

Expression

Yeast

Mutation

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-GFP-bleMX6

Plasmid

#33141

Purpose

genomic GFP tagging in yeast

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Inserts

bleMX6
GFP

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pMAM-RecG-EGFP

Plasmid

#31274

Depositor

Andrew Pierce

Article

Killen et al BMC Mol Biol. 2012 Oct 30;13:33. doi: 10

Insert

RecG-EGFP

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pBEAST-J23101-sfGFP

Plasmid

#116917

Purpose

Strong constitutive expression of super-folder GFP for cell-free expression

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Tags

Expression

Mutation

Promoter

J23101

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.SV40.eGFP.SV40(polyA)

Plasmid

#195558

Purpose

Expresses eGFP under control of short GFAP promoter

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

eGFP

Use

AAV

Tags

No tags

Expression

Mammalian

Mutation

Promoter

short GFAP

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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