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Plasmid#105187PurposeModule 3 - GAL4 synthetic promoter( with fourteen binding sites) drives mKate2DepositorInsertG14p-mKate2
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Puro-PNPT1
Plasmid#223315PurposeLentiviral vector expressing human PNPT1DepositorInsertPolyribonucleotide Nucleotidyltransferase 1 (PNPT1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_CRFmut
Plasmid#208663PurposeExpresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) control sensor GRAB_CRFmut in a cre-dependent mannerDepositorInsertGPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
UseAAVTagsExpressionMutationPromoterEFSAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-GFP (PX458)
Plasmid#48138PurposeCas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNADepositorHas ServiceCloning Grade DNAInserthSpCas9
UseCRISPRTags3XFLAG and GFPExpressionMammalianMutationPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSR646
Plasmid#65215PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-6 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-6 cap
UseTagsExpressionInsectMutationPromoterP10 and polyhedrinAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tg(mpeg1:LifeAct-mRuby)
Plasmid#166977Purposelabels F-actin in macrophagesDepositorUseTagsLifeact-mRuby and mRubyExpressionMutationPromoterAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CamKIIa_GFP
Plasmid#96941PurposeTo label mature excitatory iPSC-derived human neuronsDepositorInsertGreen fluorescent protein
UseLentiviralTagsExpressionMutationPromoterCaMKIIaAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lenti-multi-Guide
Plasmid#85401PurposeLentiviral vector for the delivery of multiple sgRNAs targeting different genes in combination with inducible Cas9 expresssion by pLenti-iCas9-neoDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lenti-multi-CRISPR
Plasmid#85402PurposeLentiviral vector for the delivery of multiple sgRNAs targeting different genes with constitutive Cas9 expressionDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
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MelanoTag-PURO (pLJC5-GPR143-mScarlet-3xHA)
Plasmid#160369PurposeStable expression of GPR143-mScarlet-3xHA fusion protein for the isolation of melanosomes.DepositorInsertGPR143 (GPR143 Human)
UseLentiviralTagsmScarlet-3xHAExpressionMutationPromoterAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
p63RhoGEF619-mVenus-N1
Plasmid#84339PurposeExpresses p63 (619 a.a.) tagged with mVenusDepositorInsertp63RhoGEF (619 aa) (ARHGEF25 Human)
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSynapsin-Cre-WPRE
Plasmid#86641PurposeExpresses Cre in mammalian cellsDepositorInsertCre
UseCre/Lox, Lentiviral, Mouse Targeting, and Synthet…TagsExpressionMammalianMutationPromoterhSynapsinAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-αTAT1[2-236]
Plasmid#27101DepositorInsertαTubulin K40 acetyltransferase (ATAT1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC_P2A_Hygro_Barcode
Plasmid#120461PurposeBarcoded lentiviral vector to express MYC in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC (MYC Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab7A
Plasmid#61803PurposeHuman Rab7A fluorescently tagged with GFP on the N-terminus for mammalian expressionDepositorInsertRab7A (RAB7A Human)
UseTags2x HA and GFPExpressionMammalianMutationNonePromoterCMVAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMVd1-EGFP-hRhoA-wt
Plasmid#133926PurposeLow level expression of GFP fused human RhoA wild typeDepositorInsertGFP-hRhoA WT (RHOA Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GCaMP3fast
Plasmid#74422PurposeExpress fluorescent calcium indicator GCaMP3fast in mammalian cellsDepositorInsertGCaMP3fast
UseTagsExpressionMammalianMutationW4Y D356APromoterCMVAvailable sinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSETB/mStayGold(c4)
Plasmid#212018PurposeFor N-terminal tagging with mStayGold. c4 is the appendage that facilitates fusions to the C-terminus of mStayGold.DepositorInsertmStayGold(c4)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE-Tight-MitoTimer
Plasmid#50547PurposeExpresses the Timer protein targeted to the mitochondria under a Tet-inducible promoterDepositorInsertMitoTimer
UseTagsExpressionMammalianMutationF12S mutation in TimerPromoterAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only