-
Plasmid#22444DepositorInsertWT eNOS-GFP
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBMN YFP-DHFR(DD)
Plasmid#29326DepositorInsertYFP-DHFR(DD)
UseRetroviralTagsHA and YFPExpressionMutationChange Arg 12 to His (R12H),Asn 18 Thr (N18T), Va…PromoterAvailable sinceApril 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-DeAct-SpvB
Plasmid#89446PurposeExpresses EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cellsDepositorInsertDeAct-SpvB (spvB Salmonella enterica)
UseTagsEGFPExpressionMammalianMutationTruncation spanning SpvB amino acids 375-591 (mon…PromoterCMVAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVenus-C1-Cdc20 (1534)
Plasmid#26058DepositorInsertCdc20 (Cdc20 Human)
UseTagsVenusExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
Reporter-2
Plasmid#180219PurposeSurrogate reporter plasmid for the assay of targeted RNA editingDepositorInsertSurrogate reporter
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.GCaMP6f.WPRE.SV40
Plasmid#100837PurposeAAV expression of ultrasensitive protein calcium sensor from Synapsin promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6f
UseAAVTagsExpressionMammalianMutationGCaMP3-T302L R303P A317E D380Y T381R S383T R392GPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC57-gCarvi-T2A
Plasmid#190333PurposeFluorescent reporter for cAMPDepositorInsertgCarvi
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
GAG-Pan
Plasmid#99640PurposeEGFP-mCherry Met71GAG reporter for methionine misincorporationDepositorInsertmCherry
UseTagsEGFPExpressionMammalianMutationMet71 mutated to GAG (Glu)PromoterCMVAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA2-CUL4A
Plasmid#19907DepositorInsertCullin 4A (CUL4A Human)
UseTags2xHAExpressionMammalianMutationK644RPromoterCMVAvailable sinceApril 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRa_all-in-one
Plasmid#183695PurposeExpresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selectiveDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBZS-M.CviPI
Plasmid#198355PurposeExpress M.CviPI in bacterial cells.DepositorInsertM.CviPI (M.CviPI )
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKM496 (deletions) Zeo
Plasmid#109301PurposeORBIT integrating plasmid for deletion of target gene, using zeocin as a selection marker.DepositorInsertBxb1 attB site
UseOrbit integrating plasmidTagsExpressionMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
WT-Pan
Plasmid#99638PurposeEGFP-mCherry control reporter for methionine misincorporationDepositorInsertmCherry
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
(114) pcDNA3.1-GFP-Flag-JunB-EPEA
Plasmid#160743PurposeExpresses a GFP tagged JunB with Flag and EPEA tags.DepositorInsertGFP-Flag-JunB-EPEA (JUN Human)
UseTagsEPEA, Flag, and GFPExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL004
Plasmid#215336PurposeamyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank-Hy promoter)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterhyper-pSpankAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
piggyBAC-H2B FR-MQV
Plasmid#194079PurposeExpresses nuclear-localized FR-MQV in mammalian cellsDepositorInsertFR-MQV
UseTagsHistone H2BExpressionMammalianMutationMethionine 42 to Glutamine, Cystiene 159 to Valin…PromoterCMV7Available sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hc-MYC
Plasmid#17220PurposeRetroviral expression of human cMYCDepositorInsertc-MYC (MYC Human)
UseRetroviralTagsExpressionMammalianMutationNonePromoterAvailable sinceFeb. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
mcherry-Myo 9b
Plasmid#134918PurposeExpression of rat Myo 9b (Myr 5) in mammalian cells.DepositorInsertMyo 9b (rat) with 3' UTR (Myo9b Rat)
UseTagsmcherryExpressionMammalianMutationPromoterCMV IEAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Desmin-N-18
Plasmid#54060PurposeLocalization: Intermediate Filaments, Excitation: 487, Emission: 509DepositorInsertDesmin (DES Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP/GCP60
Plasmid#180633PurposeExpression of GCP60DepositorInsertGCP60 (ACBD3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1
Plasmid#183951PurposeInducible lentiviral expression, TRE-control-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTADepositorInsertAPOBEC1 (Apobec1 Rat)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE HA-epitope donor ORF1
Plasmid#200372PurposeTagging endogenously expressed proteins with HA epitope, C-terminalDepositorInsertHA epitope
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_SST1.0
Plasmid#208664PurposeExpresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in mammalian cellsDepositorInsertGPCR activation based somatostatin (SST) sensor GRAB_SST1.0
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLR-NI
Plasmid#31006DepositorInsertlast NI repeat
UseTagsExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NurRE3
Plasmid#208253PurposepGL3 promoter luciferase reporter vector containing 3 copies of the NurRE POMC promoter DNA response element (5′-GATCGTGATATTTACCTCCAAATGCCA- 3′) used for luciferase reporter assays in mammalian cellsDepositorInsert3X Nur response element LUC
UseTagsNoExpressionMammalianMutationPromoterSV40 early promoterAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLR-NG
Plasmid#30995DepositorInsertlast NG repeat
UseTagsExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS/ES-EnvA-VSVg-WPRE-pA
Plasmid#87221Purposeto package wild type EnvA psudotyped lentivirusDepositorInsertEnvA-VSVg
UseMouse Targeting and Synthetic BiologyTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLJM60 mUlk1 (wt) barcoded
Plasmid#48799Purposeexpresses wt Ulk1DepositorInsertULK1 (Ulk1 Mouse)
UseLentiviralTagsFLAG and barcode: GGATCCExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
HcKCR2_mCherry_pcDNA3.1
Plasmid#177337PurposeExpresses KCR2 in mammalian cellsDepositorInsertHcKCR2
UseTagsmCherryExpressionMammalianMutationmammalian codon-adaptedPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FAST-p10
Plasmid#89152PurposeExpress p10-FAST in mammalian cellsDepositorInsertNBV p10-FAST
UseTagsExpressionMammalianMutationPromoterCAG promotorAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
SF-SON IRES Puro
Plasmid#214402PurposeExpresses human SOX10, OLIG2, NKX6.2 and PAC (puromycin N-acetyl-transferase)DepositorUseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9
Plasmid#84245PurposeExpresses direct fusion KRAB-Sp dCas9DepositorInsertsKRAB-tagBFP-Sp dCas9
Puro
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), HA tag, KRAB, and tagBFPExpressionMammalianMutationPromoterCAG and PGKAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSetB-Peredox
Plasmid#73806PurposeExpresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in bacteria, with N-terminal his-tagDepositorInsertPeredox
UseTagshis-tag to facilitate purificationExpressionBacterialMutationPromoterT7Available sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pR562
Plasmid#207886PurposeCreation of Level 0 entry clonesDepositorInsertamilCP
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
GAA-Pan
Plasmid#99639PurposeEGFP-mCherry Met71GAA reporter for methionine misincorporationDepositorInsertmCherry
UseTagsEGFPExpressionMammalianMutationMet71 mutated to GAA (Glu)PromoterCMVAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLR-HD
Plasmid#30984DepositorInsertlast HD repeat
UseTagsExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLL8 lenti-D10Acas9-3xflag-blast
Plasmid#112134Purposelenti vector encoding D10Acas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-D10ACas9-3Xflag-T2A-Blast-WPRE)DepositorInsertD10Acas9-3xflag-blast
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A mutant in Cas9PromoterEF1aAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_Flag_TP53_R248W
Plasmid#183114PurposeExpresses flag-tagged p53 with R248W mutation in mammalian cellsDepositorInsertTP53 (TP53 Human)
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationchanged arginine 248 to tryptophanPromoterEF1aAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Matr3 delRRM2
Plasmid#32882DepositorInsertMATR3 Matrin3 (MATR3 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationdeleted amino acids 497-566PromoterCMVAvailable sinceJan. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Zeo
Plasmid#75088Purposeretrovirus-mediated gene transfer into mammalian cellsDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLR-NN
Plasmid#31017DepositorInsertlast NN repeat
UseTagsExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-PCNA-IRES-puro2b
Plasmid#26461DepositorInsertPCNA (PCNA Human)
UseTagspEGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(0)-T2A-Gal4
Plasmid#62899PurposeCreating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 0DepositorInsertT2A-Gal4
UseTagsExpressionMutationPromoterAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
lenti-SpCas9 puro
Plasmid#104994PurposeThis 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
RAB11A-GFP HDRT Source (pTR 143)
Plasmid#112012PurposeDNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with GFPDepositorInsertRAB11A-GFP HDRT (RAB11A Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJJiaDEST-APEX2-GAL9
Plasmid#149520PurposeExpress APEX2-Galectin-9 in mammalian cellsDepositorInsertLGALS9 (LGALS9 Human)
UseTagsAPEX2ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CWB-pre-eGRASP(p32)
Plasmid#111598PurposeAn AAV vector that expresses green pre-eGRASP(p32) under the CaMKIIa promoter.DepositorInsertgreen pre-eGRASP(p32)
UseAAVTagsExpressionMutationPromoterCaMKIIaAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Neo DEST (w531-1)
Plasmid#19067Purpose3rd gen lentiviral Gateway destination vector, expression, PGK promoter, NeoDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZAP70
Plasmid#23887DepositorInsertZAP70 (ZAP70 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GST-AGO1
Plasmid#24316DepositorInserthuman AGO1 (AGO1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceApril 7, 2010AvailabilityAcademic Institutions and Nonprofits only