Showing: 4701 - 4720 of 18079 results
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Plasmid#61254PurposeTemplate for cloning sgRNA(F+E) to generate new PU6::sgRNAs by PCR-fusionDepositorInsertsgRNA(F+E) targeting Y61A9L9.1
UseCRISPR; Cloning templateTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-0-2xTY1-V5
Plasmid#61255PurposeCRISPR-RMCE, second step attB plasmid, intron phase 0, exchange dsRed with 2xTY1 and V5 tags cassetteDepositorInsert2xTY1-V5
UseCRISPRTagsTY1 and V5ExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCM433kanT
Plasmid#61262PurposeSucrose counterselection (sacB) vector for unmarked allelic exchange.DepositorTypeEmpty backboneUseSynthetic Biology; Sucrose counterselectionTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAWP78
Plasmid#61263PurposeSmall IncP-based broad host range replicating vector.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAWP89
Plasmid#61264PurposeIncP-based replicating vector expressing dTomato under tac promoter.DepositorInsertPtac-dTomato
UseSynthetic BiologyTagsExpressionBacterialMutationPromotertacAvailabilityAcademic Institutions and Nonprofits only -
pMM178
Plasmid#61270PurposeAlso referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.DepositorInsertCas9, tracrRNA, crRNA
UseCRISPRTags6xHisExpressionBacterialMutationPromoterpLtetO, native, native (respectively)AvailabilityAcademic Institutions and Nonprofits only -
pMM441
Plasmid#61271PurposeAlso referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.DepositorInsertCas9, tracrRNA, crRNA
UseCRISPRTags6xHisExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pRC319
Plasmid#61272PurposeAlso referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.DepositorInsertCas9, tracrRNA, crRNA
UseCRISPRTags6xHisExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
NOVA1 eGFP
Plasmid#61275PurposeGFP-tagged ORF clone of Homo sapiens neuro-oncological ventral antigen 1 (NOVA1), transcript variant 1DepositorInsertNOVA1 (NOVA1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pDest eGFP CUGBP1
Plasmid#61276PurposeMammalian expression of EGFP-tagged human CUGBP1DepositorInsertCUG-BP (CELF1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pDest eGFP MBNL1 42 kDa
Plasmid#61277PurposeMammalian expression of EGFP-tagged human MBNL1DepositorInsertMBNL1 (MBNL1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
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pmIL-6 mut NF-kB
Plasmid#61293Purposedrives luciferase from mouse IL-6 promoter with mutation in NF-kB siteDepositorInsertIL-6 promoter (Il6 Mouse)
UseLuciferaseTagsExpressionMammalianMutationMutated NF-kB binding site from GTGGGATTTTCCCA to…PromoterIL-6 promoter with mutant NF-kB binding siteAvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N3 alpha-SSeCKS
Plasmid#61294PurposeMammalian expression of GFP-alpha-SSeCKSDepositorInsertalpha SSeCKS (Akap12 Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCWori cytochrome P450-BM3h 9D7
Plasmid#61308PurposeExpresses Cytochrome P450-BM3h 9D7 with C-terminal 6-His tagDepositorInsertCytochrome P450-BM3 heme domain 9D7
UseTags6xHisExpressionBacterialMutationT268A, I263A, T438V, A328GPromoterTacAvailabilityAcademic Institutions and Nonprofits only -
pDN34
Plasmid#61343PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/PKIt NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-biNLS2 and PKIt NESExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pDN77
Plasmid#61347PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkBα NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-biNLS2 and NES IkBaExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pDN80
Plasmid#61349PurposeLINuS-flex: modular LINuS construct enabling introduction of any protein encoding sequence, NES and NLS. The sequence of interest replaces the LexA DBD-MBP-VP64 fragment.DepositorTypeEmpty backboneUseTagsAsLOV2 wt and mCherryExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-HA
Plasmid#61355Purposeencodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal HA tag
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-FLp300
Plasmid#61356Purposeencodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 Human, Synthetic, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 4701 - 4720 of 18079 results