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AP-SEMA3A (chick)

Plasmid

#29448

Depositor

Jonathan Raper

Article

Feiner et al Neuron. 1997 Sep . 19(3):539-45.

Insert

AP-SEMA3A (chick) (SEMA3A Chicken)

Use

Tags

AP tagged

Expression

Mammalian

Mutation

Promoter

Available since

July 1, 2011

Availability

Academic Institutions and Nonprofits only
psgRNAc

Plasmid

#114006

Purpose

express sgRNA, p15A, CRM

Depositor

David Bikard

Article

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

BBa_J23119

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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GolgiCKAR

Plasmid

#14871

Depositor

Alexandra Newton

Article

Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

Insert

C kinase activity reporter

Use

Tags

CFP, YFP, and eNOS(1-33)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
pcS-mCherry-C1C2

Plasmid

#178425

Purpose

Fluorescence reporter fused to EV membrane binding domain for EV imaging and tracking

Depositor

Masako Harada

Article

Komuro et al Nanotheranostics. 2021 Apr 15;5(4):378-3

Insert

mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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NucCKAR

Plasmid

#14869

Depositor

Alexandra Newton

Article

Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

Insert

C kinase activity reporter

Use

Tags

CFP, NLS, and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
CMVTO-Gbp6-TM-Che

Plasmid

#180022

Purpose

Membrane-bound anti-GFP nanobody conjugated to mCherry

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Gpb6 nanobody

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pMA3211

Plasmid

#46879

Purpose

Lentiviral vector for Tet-inducible expression. Puromycin-resistance

Depositor

Troy Stevens, Mikhail Alexeyev

Article

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE-Tight

Available since

Aug. 14, 2013

Availability

Academic Institutions and Nonprofits only

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m.Setd7

Plasmid

#87131

Purpose

mammalian expression of Setd7

Depositor

Francesca Spagnoli

Article

Kofent et al Development. 2016 Oct 1;143(19):3573-358

Insert

Setd7 (Setd7 Mouse)

Use

Tags

6x-Myc

Expression

Mammalian

Mutation

Promoter

simian CMV IE94

Available since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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LentiGuide-BC-start

Plasmid

#127168

Purpose

Entry plasmid for two-step guide-barcode cloning

Depositor

Paul Blainey

Article

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

N/A

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLRD04

Plasmid

#208258

Purpose

Production of ent-kaurene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
ptmT2
blr2150

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCB1532S-RFP

Plasmid

#101856

Purpose

Golden-Gate compatible fungal transformation vector carrying Sulfonylurea Resistance (Chlorimuron Ethyl) with RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)

Depositor

Sophien Kamoun

Article

Pennington et al Figshare (2017)

Type

Empty backbone

Use

Fungal expression plasmid, tested in magnaporthe

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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CMVTO-KRAS-G12V

Plasmid

#179654

Purpose

KRAS active mutant (G12V mutation)

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

KRAS G12V

Use

Tags

Expression

Mammalian

Mutation

G12V

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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mSc-sspB

Plasmid

#114421

Purpose

Translocating to iLID domain

Depositor

Dorus Gadella

Article

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

iLID domain

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pLRD11

Plasmid

#208263

Purpose

Production of sclareol in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
abCAS
ssSS

Use

Tags

Expression

Bacterial

Mutation

abCAS(D537A)

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDRESS_mTurquoise2_spatial-linker-P2A_mScarletI

Plasmid

#130510

Purpose

expresses mScarlet1

Depositor

Dorus Gadella

Article

Bindels et al Nat Protoc. 2020 Feb;15(2):450-478. doi:

Insert

mScarlet1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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CMVTO-KRAS-G12D

Plasmid

#179656

Purpose

KRAS active mutant (G12D mutation)

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

KRAS G12D

Use

Tags

Expression

Mammalian

Mutation

G12D

Promoter

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR PKAKTRn-Clover

Plasmid

#177199

Purpose

Gateway entry vector encoding PKAKTRnew-Clover

Depositor

Markus Covert

Article

Kudo et al Cell Syst. 2022 May 18;13(5):376-387.e8.

Insert

PKAKTRnew-mClover

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pFlare9A-Dup34

Plasmid

#90267

Purpose

Minigene reporter for alternative splicing analysis

Depositor

Douglas Black

Article

Stoilov et al Proc Natl Acad Sci U S A. 2008 Aug 12;10

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK021-7-PARS

Plasmid

#84990

Purpose

Type 7, Pichia autonomously replicating sequence

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Pichia autonomously replicating sequence

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Non

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCI 9.0

Plasmid

#74229

Purpose

Luminescent coincidence reporter with FLAG tags, FLuc-P2A-NLucPEST

Depositor

James Inglese

Article

Schuck et al Curr Protoc Neurosci. 2017 Apr 10;79:5.3

Insert

3xFlag-FLuc-P2A-3xFlag-NLucPEST

Use

Tags

Expression

Mammalian

Mutation

Promoter

None

Available since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

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