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Plasmid#131611Purposeexpression of mouse Gfi1b in mammalian cellsDepositorInsertGfi1b (Gfi1b Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hLIN28A
Plasmid#47902PurposeExpresses human LIN28A in mammalian cells using retrovirus system.DepositorInserthuman LIN28A (LIN28A Human)
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceOct. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGFP-C-IFT81
Plasmid#218732PurposeExpresses N-terminally EGFP-tagged IFT81 in mammalian cellsDepositorInsertIFT81 (IFT81 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
p-AAV-sh[SNCA]
Plasmid#75437Purposeknockdown alpha-synuclein in rat neuronsDepositorInsertsnca (Snca Rat)
UseAAV and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
HiBiT-FZD5
Plasmid#195843PurposeExpresses human FZD5 with an N-terminal HiBiTDepositorInsertFZD5 (FZD5 Human)
UseTagsHiBiTExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
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LUC01 (L/Sec/P)
Plasmid#112144PurposeMammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activityDepositorInsertLuciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Rat, Photinus pyralis)
UseTagsExpressionMammalianMutationCysteine 258 mutated to selenocysteine (U)PromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB2904(XI-3 MarkerFree)
Plasmid#73276PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-3 (Chr XI: 93378..94567)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO-TRE-AFOS
Plasmid#197079PurposeA doxycycline inducible dominant negative form of transcription complex AP1. AFOS heterodimerizes and inhibits c-Jun. Plasmid contains PGK driven H2B-RFP as a fluorescent reporter of transduction.DepositorInsertAFOS (Fos Mouse)
UseLentiviralTagsFlag and T7ExpressionMutationPromoterTREAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS mSynCAM1
Plasmid#115496Purposeexpression of mouse full-length SynCAM 1, CAG promoter suited for neuronal expressionDepositorInsertCadm1 (Cadm1 Mouse)
UseTagsExpressionMammalianMutationPromoterchicken beta-actinAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-Cep123 (CCDC123/Cep89)
Plasmid#67787PurposeThis plasmid encodes EGFP fused to the N-terminus of isoform 1 of Cep123 (CCDC123/Cep89)DepositorInsertCep123 (CEP89 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgMyc_1
Plasmid#138324PurposeExpress guide RNA 2 for mouse MycDepositorInsertcMyc sgRNA (Myc Synthetic)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
LV-Let7d-5p
Plasmid#117055PurposeOverexpression of Let-7d-5p using miR-451 backbone to enable DICER independent expression along with orange fluorescent protein (OFP) to track miRNA expressionDepositorInsertLet-7d-5p - OFP
UseLentiviralTagsExpressionMutationPromoterSFFV promoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pCG-TPMV Hd32-IL-13
Plasmid#163869PurposeExpresses TPMV H glycoprotein bearing IL-13 in mammalian cellsDepositorInsertTPMV H glycoprotein/IL-13
UseTagsHuman IL-13ExpressionMammalianMutationAmino acids 2 to 33 at N terminus deletedPromoterAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pMyc-C1-Cep123 (isoform 3)
Plasmid#73328PurposeEncodes isoform 3 of Cep123 fused to a myc tagDepositorInsertCep123 (CEP89 Human)
UseTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMyc-C1-Cep123 (isoform 2)
Plasmid#73327PurposeEncodes isoform 2 of Cep123 (Cep89/CCDC123) fused to a myc tagDepositorInsertCep123 (CEP89 Human)
UseTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pColdI-GST-mGTSF1l
Plasmid#214817PurposeBacterial expression of GST-tagged mouse GTSF1lDepositorInsertGTSF1l (Gtsf1l Mouse)
UseTags6x His and GSTExpressionBacterialMutationPromoterCspAAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
gZiPro
Plasmid#198441PurposeActivity/inhibition assays, 19F-NMRDepositorInsertglycine linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationR95*A,PromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
LUC05 (L/Cys/P)
Plasmid#112146PurposeMammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activityDepositorInsertLuciferase coding region with wild type Cysteine 258 and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Rat, Photinus pyralis)
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgMyc_2
Plasmid#138346PurposeExpress guide RNA 1 for mouse MycDepositorInsertcMyc sgRNA (Myc Synthetic)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEB30 (hANGPTL8-V5-His)
Plasmid#109114PurposeExpresses V5 and His-tagged human ANGPTL8 in mammalian cellsDepositorInsertANGPTL8 (ANGPTL8 Human)
UseTags6x His and V5ExpressionMammalianMutationPromoterCMVAvailable sinceMay 21, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
139H2_LC
Plasmid#206202PurposeFor recombinant expression of the light chain of the Mouse anti-MUC1 antibody 139H2 in mammalian cells.DepositorInsertanti-MUC1 antibody 139H2 light chain (Igkc Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pLC-Flag-CSNK1A1 G40N-Puro
Plasmid#123320PurposeLentiviral vector for expression of Flag tagged CK1alpha G40N-P2A-Puro casette from a CMV promoterDepositorInsertCSNK1A1 (CSNK1A1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationchanged Glycine 40 to AsparaginePromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ana GvpC-SpyTag-His in pET28a
Plasmid#85737PurposeExpresses His-tagged GvpC from Anabaena flos-aquae fused to SpyTag in E.coliDepositorInsertGas vesicle protein C (fused to SpyTag and His-tagged)
UseTagsHis-tag and SpyTagExpressionBacterialMutationAdded an extra glycine after the methionine at th…PromoterT7Available sinceMarch 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
HypaCas9
Plasmid#138557PurposeExpresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSDDepositorInsertHypaCas9
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationN692A, M694A, Q695A, H698APromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
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p20StAD
Plasmid#190268PurposeConstitutively expresses a protein of interest fused to a GAL4 transcription activating domain. Targeted integration to genomic locus YPRC-delta15, Chromosome XVI.DepositorTypeEmpty backboneUseSynthetic Biology; Yeast genome-integrating vectorTagsGAL4-ADExpressionMutationPromoterTDH3Available sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pH2R-P2A-mCherry-N1
Plasmid#84331PurposeCo-expresses (untagged) Histamine-2 receptor and mCherryDepositorInsertH2R-P2A-mCherry (HRH2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB3035(X-4 MarkerFree)
Plasmid#73273PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-4 (Chr X: 236336..237310)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB3034(X-3 MarkerFree)
Plasmid#73272PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-3 (Chr X: 223616..224744)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
mIFP24-C1
Plasmid#54820PurposeLocalization: C1 Cloning Vector, Excitation: 683, Emission: 704DepositorTypeEmpty backboneUseTagsmIFP24ExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLV-NT-3/D15A
Plasmid#73035PurposeExpress an NT-3-BDNF hybrid neurotrophic factor in mammalian cellsDepositorInsertNT-3/D15A (NTF3 Human)
UseLentiviralTagsExpressionMammalianMutationMutated Aspartic acid in position 15 of the matur…PromoterAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pCALNL-Kir2.1
Plasmid#125578PurposeCre-dependent expression of Kir2.1 (FLAG-tagged) in mammalian cells.DepositorInsertKir2.1 (Kcnj2 Mouse)
UseCre/LoxTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
mIFP-Lysosomes-20
Plasmid#56228PurposeLocalization: Lysosome Membrane, Excitation: 683, Emission: 704DepositorInsertLAMP1
UseTagsmIFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pR I-Ab alpha BirA
Plasmid#113638PurposeInsect cell expression vector for I-Ab alpha chain fused to BirA biotinylation siteDepositorInsertI-Ab alpha BirA (H2-Ab1 Mouse)
UseTagsBirA biotinylation signal site and fused to Fos-J…ExpressionInsectMutationtransmembrane and cytoplasmic domains truncatedPromoterMetallothioneinAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pR2Q347y
Plasmid#112680Purposefunctional, fluorescent, glutamate receptor subunitDepositorInsertGlutamate receptor subunit (Gria2 Rat)
UseTagsfluorescent proteinExpressionMammalianMutationtagged with fluorescent proteinPromotercmVAvailable sinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBtPIPLC His
Plasmid#173808PurposeExpression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cellsDepositorInsertBacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
UseTags6XHisExpressionBacterialMutationnonePromoterT7Available sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
hsDCX-EGFP-pHAT
Plasmid#83918Purposehuman Doublecortin, N-terminal 6-His tag, C-terminal EGFP-Strep-tag IIDepositorInsertDoublecortin isoform b (DCX NM_178152.2, Human)
UseTags6-His and EGFP+Strep-tag IIExpressionBacterialMutationPromoterAvailable sinceDec. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-ligase-NLS-cTF
Plasmid#87746PurposeIPTG-inducible expression of ligase-NLS-cTF fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHis and NLS linker - cTF (chimeric transcriptio…ExpressionBacterialMutationPromoterT5-lacAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCfB2899(X-2 MarkerFree)
Plasmid#73271PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-2 (Chr X: 194944..195980)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-tev-std
Plasmid#109423PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, and T7ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pH3R-P2A-mCherry-N1
Plasmid#84332PurposeCo-expresses (untagged) Histamine-3 receptor and mCherryDepositorInsertH3R-P2A-mCherry (HRH3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG32-3HA-3FLAG
Plasmid#154081PurposeFor C-terminal 3xHA-3xFLAG-protein tagging in S. pombe using hphMX4 as resistance marker.DepositorInsert3xHA-3xFLAG-hygR
UsePcr-based tagging in yeastTags3xHA-3xFLAGExpressionMutationPromoterAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-DroshaS300/302A
Plasmid#62530PurposeSerines 300 and 302 of Drosha are mutated to alaninesDepositorInserthuman Drosha with serines 300 and 302 changed alanines (DROSHA Human)
UseTagsGFPExpressionMammalianMutationchanged serines 300 and 302 to alaninesPromoterAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only