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Plasmid#144376PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertKDR (KDR Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T Easy-AtpU6-26
Plasmid#160218PurposeAtpU6-26 Golden Gate level 0 piece to express gRNAsDepositorInsertpAtU6-26 promoter
UseGolden gate level 0 piece to express grnasTagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mDrc7-FLAG
Plasmid#176470PurposeExpression vector of mouse dynein regulatory complex subunit 7 (Drc7) tagged with FLAG at C-terminus.DepositorInsertdynein regulatory complex subunit 7 (Drc7 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCAG promoterAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mTcte1-PA
Plasmid#176469PurposeExpression vector of mouse t-complex-associated testis expressed 1 (Tcte1) tagged with PA at C-terminus.DepositorInsertt-complex-associated testis expressed 1 (Tcte1 Mouse)
UseTagsPAExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYeDP60-hATP13A2-E343A
Plasmid#171378PurposeA modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2 E343A mutant, followed by a thrombin cleavage site and a C-term BAD tag. DOI: 10.21769/BioProtoc.3888DepositorInserthuman ATP13A2 variant 2 wild-type
UseTagsBAD tagExpressionYeastMutationE343APromoterURA3Available sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-miRFP670-pA_U6-BbsI (pE059)
Plasmid#149353PurposeMammalian expression vector for miRFP670DepositorInsertmiRFP670
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCri_L830-antiSH2-OptoMB-V416L
Plasmid#159936PurposeOpto-MB bacterial expressionDepositorInsertanti-SH2 MB with SS58 insert
UseTagsExpressionBacterialMutationAsLOV2 V416LPromoterAvailable sinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCri_L889-antiSH2-OptoMB-Triple
Plasmid#159937PurposeOpto-MB bacterial expressionDepositorInsertanti-SH2 MB with SS58 insert
UseTagsExpressionBacterialMutationAsLOV2 V416I_G528A_N538EPromoterAvailable sinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBW1340_pCAG-PV1-PhiC31-N233-L1-FRB-NLS-BGHpA
Plasmid#108789PurposeExpresses N-terminus to aa233 of PhiC31 integrase fused to FRB CIDDepositorInsertPhiC31 integrase fragment N-terminus to aa233
UseCre/Lox and Synthetic BiologyTagsFRB,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1353_pCAG-PV1-FKBP-L1-PhiC31-234C-NLS-BGHpA
Plasmid#108790PurposeExpresses aa234 to C-terminus of PhiC31 integrase fused to FKBP CIDDepositorInsertPhiC31 integrase fragment aa234 to C-terminus
UseCre/Lox and Synthetic BiologyTagsFKBP and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeAQ482A
Plasmid#128945Purposeexpresses Q482A mutant of PfeA in E. coliDepositorInsertferric enterobactin receptor
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75), …PromoterT7Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV300
Plasmid#110720PurposeLentiviral system for reduced ectopic expression.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHL 1075
Plasmid#53004PurposepCon:fhlA::mCherry, pLlacO-1:sodB::gfpDepositorInsertsfhlA
sodB
UseSynthetic BiologyTagsGFP and mCherryExpressionBacterialMutationcontains −309 to +96 bp relative to start codon a…PromoterpCon and pLlacO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV V5-LUC Blast (w567-1)
Plasmid#21474Purpose3rd gen lentiviral V5-Luciferase expression vector, CMV promoter, BlastDepositorInsertV5-LUC
UseLentiviral and LuciferaseTagsV5ExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-FLAG
Plasmid#119759PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal FLAG tag.DepositorTypeEmpty backboneUseTagsFLAG and GSTExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-SopF
Plasmid#135174PurposeExpresses an mCherry-SopF fusion in mammalian cellsDepositorInsertSopF (STM1239 Salmonella enterica serovar Typhimurium)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-TEV-C9R
Plasmid#118754PurposeTEV-C9R protease expression vector with enhanced expression, solubility and yield without affecting TEV's protease activityDepositorInsertTobacco Etch Virus protease (NEWENTRY Synthetic)
UseTagsC9R and His tagExpressionBacterialMutationPromoterT7Available sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPomyces-2
Plasmid#61737PurposeStreptomyces expression of codon-optimized Cas9 and custom gRNADepositorInsertssSpCas9
gRNA cassette
UseCRISPRTagsExpressionBacterialMutationBbsI-flanked lacZ cassette inserted in place of s…Promotergapdhp(EL) and rpsL(XC)-BbsIAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Caveolin-C-10
Plasmid#55008PurposeLocalization: Membrane, Excitation: 587, Emission: 610DepositorInsertCaveolin (CAV1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCFD5
Plasmid#73914Purposemultiplex gRNA plasmidDepositorInsertdU6-3:tRNA:gRNA:tRNA:gRNA
UseCRISPRTagsExpressionInsectMutationPromoterdU6:3Available sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only