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We narrowed to 8,972 results for: yeast

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ampl43

Plasmid

#161830

Purpose

pRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.

Depositor

Saeed Tavazoie

Article

Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a-trAtCCD8(pYL735)

Plasmid

#178286

Purpose

Expresses trAtCCD8 in E. coli. pET21a carrying N-terminus 56 amino-acid truncated CCD8 from Arabidopsis

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

trAtCCD8

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pZS165 CRISPEY HH-HDV NotI

Plasmid

#114451

Purpose

Yeast Centromere Plasmid for galactose inducible expression of retron-guide chimeric RNA with flanking HH-HDV ribozyme

Depositor

Hunter Fraser

Article

Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

Type

Empty backbone

Use

CRISPR; Cen/ars

Tags

Expression

Yeast

Mutation

Promoter

ScGAL7p

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pRS402-nicastrin/APH-1a-HA

Plasmid

#207804

Purpose

Yeast integrative vector for expression of Nicastrin and APH-1a-HA

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Gamma-secretase subunits nicastrin and Aph1a

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pDZ585 pKAN 2x-mCherry

Plasmid

#72236

Purpose

Floxed Kanamycin (G418) selection maker; 3' tagging vector used to create C-terminal fusion proteins with 2x-mCherry

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

2x-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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PTEF1-yEGFPCLN2PEST-pRS406

Plasmid

#64406

Purpose

TEF promoter driven GFP expression

Depositor

Claudia Vickers

Article

Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

Insert

TEF1 promoter (TEF1 Budding Yeast)

Use

Tags

yEGFPCLN2PEST (destabilized yeast EGFP)

Expression

Yeast

Mutation

Promoter

TEF1 promoter

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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p404-MTS-mCherry-GFP1-10

Plasmid

#91958

Purpose

Expresses mCherry-GFP1-10 in yeast cell mitochondria

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

MTS-mCherry-GFP1-10

Use

Tags

Expression

Yeast

Mutation

Promoter

GAP

Available since

June 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCORE-Kp53

Plasmid

#72241

Purpose

Contains the CORE cassette with KanMX4 and human p53 gene markers

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Genet Eng (N Y). 2003;25:189-207.

Inserts

p53 (TP53 Human)
kanMX4

Use

Tags

Expression

Bacterial

Mutation

V122A mutation

Promoter

Available since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pJOG417

Plasmid

#105341

Purpose

Yeast two hybrid

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pJOG418

Plasmid

#105342

Purpose

Yeast two hybrid

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-ANP1-msGFP2

Plasmid

#177100

Purpose

Expresses Anp1-msGFP2 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

ANP1 (ANP1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pRS406-PHO5-GFP-hFAPP1 PH domain

Plasmid

#58723

Purpose

Expresses GFP-Tagged human FAPP1 PH domain in yeast

Depositor

Tim Levine

Article

Levine et al Curr Biol. 2002 Apr 30;12(9):695-704.

Insert

Four-phosphate-adaptor protein 1 PH domain (PLEKHA3 Human)

Use

Tags

GFP and Myc

Expression

Yeast

Mutation

Promoter

PHO5

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
FRP1640_PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

Plasmid

#58440

Purpose

Cassette to replace yeast promoters with an inducible promoter containing 2 lexA boxes

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

Use

Tags

Expression

Yeast

Mutation

Promoter

PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pAGM9121_His2-GFP11

Plasmid

#153511

Purpose

His2-GFP11 C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

His2-GFP11

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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FRP1639_PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

Plasmid

#58439

Purpose

Cassette to replace yeast promoters with an inducible promoter containing 1 lexA box

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

Use

Tags

Expression

Yeast

Mutation

Promoter

PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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dCas9v2

Plasmid

#103140

Purpose

TetR inducible dCas9-VPR plasmid with pRPR-NotI-tRPR1 Csy4-ready site

Depositor

Jens Nielsen

Article

Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

Insert

dCas9-VPR

Use

Tags

Expression

Yeast

Mutation

Removed scaffold gRNA from original plasmid

Promoter

Available since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCSN075

Plasmid

#166732

Purpose

Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiae

Depositor

Rene Verwaal

Article

Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

Insert

dCas12a-NLS-Mxi1

Use

CRISPR

Tags

Mxi1 and SV40 NLS

Expression

Yeast

Mutation

E925A

Promoter

S. cerevisiae TEF1

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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YIPlac204TKC-E2-Crimson-HDEL

Plasmid

#38771

Depositor

Benjamin Glick

Article

Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

Insert

E2-Crimson

Use

Tags

Linker peptide and yeast ER retention signal sequ…

Expression

Yeast

Mutation

Promoter

TPI1

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pZS160 CRISPEY HDV-HDV NotI

Plasmid

#114453

Purpose

Yeast Centromere Plasmid for galactose inducible expression of retron-guide chimeric RNA with flanking HDV-HDV ribozyme

Depositor

Hunter Fraser

Article

Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

Type

Empty backbone

Use

CRISPR; Cen/ars

Tags

Expression

Yeast

Mutation

Promoter

ScGAL7p

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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Dhh1-EGFP

Plasmid

#201418

Purpose

Bacterial protein expression of Dhh1

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dhh1 (DHH1 Budding Yeast)

Use

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

Promoter

tac

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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