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Plasmid#49132PurposeAAV plasmid with NOV (Ple303) promoter driving expression of iCre.DepositorInsertssAAV-Ple303-iCre
UseAAVTagsExpressionMammalianMutationPromoterNOVAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS2050
Plasmid#49135PurposeAAV plasmid with CAGGS promoter driving expression of EmGFP.DepositorInsertssAAV-CAGGS-emGFP
UseAAVTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
hSyn-FAS-DO-ReaChR-mCitrine-WPRE
Plasmid#64035PurposeExpresses ReaChR under hSyn promoter. FAS-DO turns off expression in Cre-positive cells. FAS is compatible with other traditional Flex/DIO constructsDepositorInserthSyn-FAS-DO-ReaChR-mCitrine-WPRE
UseAAVTagsReaChR tagged with mCitrineExpressionMammalianMutationPromoterhSynAvailable sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP pA_EB_CAGW
Plasmid#92154PurposeAAV construct encoding GFP controlDepositorInsertEGFP
UseAAVTagsExpressionMammalianMutationPromoterCAGWAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAM/CBA-eGFP-miRaSyn(mouse)x3-WPRE-bGHpA
Plasmid#194248PurposeExpresses 3 miRNAs targeting mouse alpha-SynucleinDepositorInsertmiRaSynuclein(mouse) (Snca Mouse)
UseAAV and RNAiTagsExpressionMutationPromoterCAGAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
EF1a-DIO-PSAML-141F,Y115F- GlyR-2A-FusionRed
Plasmid#226590PurposePharmacologically Selective Actuator Module, chimeric ligand-gated ion channels that can be used to manipulate electrophysiological activity.DepositorInsertEF1a-DIO-PSAML-141F,Y115F- GlyR-2A-FusionRed
UseAAVTagsFusion RedExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEARBOCS-v0
Plasmid#196495PurposeCRISPR tool to target astrocytesDepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pgfaABC1D-mCherry-CAAX
Plasmid#196488PurposeTo over express mCherry-CAAX under gfaABC1D promoter in astrocytesDepositorInsertmCherry-CAAX
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEARBOCS-v0-Gfap-KO
Plasmid#196489PurposeTo KO Gfap in mouse astrocytesDepositorInsertgRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
v3em-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP
Plasmid#207863PurposeAAV genome encoding C-terminal PE6d and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNADepositorInsertNpuC-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP
UseAAVTagsExpressionMutationSee ManuscriptPromoterCbhAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
v3em-Cterm-PE6d-dualU6-Rosa26-twinPE-attB
Plasmid#207860PurposeAAV genome encoding C-terminal PE6d and U6 expression cassettes for Rosa26 twinPE pegRNA pairsDepositorInsertNpuC-Cterm-PE6d-dualU6-Rosa26-twinPE-attB
UseAAVTagsExpressionMutationSee ManuscriptPromoterCbhAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-Terminal Split Cas9 with GyrA intein
Plasmid#58694PurposeExpresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 productionDepositorInsertHumanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
UseAAV and CRISPRTagsGyrA Csplit Intein and NLSExpressionMammalianMutationPromoterCBhAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
N-Terminal Split Cas9 with GyrA intein
Plasmid#58693PurposeExpresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 productionDepositorInsertHumanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
UseAAV and CRISPRTags3xFlag, GyrA Nsplit Intein, and NLSExpressionMammalianMutationPromoterCBhAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
v3em-Cterm-PE6c-dualU6-Rosa26-twinPE-attB
Plasmid#207861PurposeAAV genome encoding C-terminal PE6c and U6 expression cassettes for Rosa26 twinPE pegRNA pairsDepositorInsertNpuC-Cterm-PE6c-dualU6-Rosa26-twinPE-attB
UseAAVTagsExpressionMutationSee ManuscriptPromoterCbhAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pEMS2113
Plasmid#49138PurposeAAV plasmid with FEV (Ple67) promoter driving expression of EmGFP. Contains WPRE.DepositorInsertssAAV-Ple67-emGFP-WPRE
UseAAVTagsExpressionMammalianMutationPromoterFEVAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS1991
Plasmid#49122PurposeAAV plasmid with FEV (Ple67) promoter driving expression of iCre. Contains WPRE.DepositorInsertssAAV-Ple67-iCre-WPRE
UseAAVTagsExpressionMammalianMutationPromoterFEVAvailable sinceMay 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pZac2.1 EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2
Plasmid#78607PurposeA single vector AAV-Cas9 system containing SaCas9 under EFs promoter, gRNAs targeting Dmd introns 22 and 23DepositorInsertEFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2
UseAAV and CRISPRTagsHA tagExpressionMammalianMutationPromoterEF1a-short; U6Available sinceSept. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
hSyn1-AsCpf1(RR)
Plasmid#109325PurposeAAV vector expressing AsCpf1 (RR variant)DepositorInserthAsCpf1(RR)
UseAAVTagsHA and NLSExpressionMutationS542R/K607RPromoterhSyn1Available sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only