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pCEP-Puro-TetOn-L1RP-ORF1_mNeonGreen2

Plasmid

#202566

Purpose

Expresses an endogenous-like L1RP LINE-1 element with a C-terminal mNeonGreen2 fluorophore on the ORF1 protein for fluorescence microscopy

Depositor

Liam Holt

Article

Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

Insert

LINE-1

Use

Tags

mNeonGreen2 on ORF1p

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-6xHis-BglII

Plasmid

#196654

Purpose

Derived from pGLO (BioRad) by the insertion of a 6xHis Tag and a BglII site

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Type

Empty backbone

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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GST-mCh-FYVE

Plasmid

#192289

Purpose

Expresses GST- and mCherry-tagged HRS FYVE in e Coli

Depositor

James Hurley

Article

Jensen et al Sci Adv. 2022 Dec 14;8(50):eadd1436. doi

Insert

HRS FYVE domain

Use

Tags

GST-TEV-mCherry

Expression

Bacterial

Mutation

Promoter

tac

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pRR-MR-5Z

Plasmid

#23059

Depositor

Charles Miller

Article

Miller et al Plasmid. 2009 Dec 3. ():.

Insert

MR (NR3C2 Human)

Use

Tags

Expression

Yeast

Mutation

N528H, Q814R, H993L

Promoter

Available since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

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ACS1

Plasmid

#13746

Depositor

John Denu

Article

Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

Insert

Acetyl CoA Synthetase 1 (Acss2 Mouse)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 PRDM16

Plasmid

#15503

Depositor

Bruce Spiegelman

Article

Seale et al Cell Metab. 2007 Jul . 6(1):38-54.

Insert

PRDM16 (Prdm16 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

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pMXs-Hu-L-Myc

Plasmid

#26022

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Jul 26. (

Insert

L-Myc (MYCL Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pSFFV_sfCherry2_TagBFP

Plasmid

#83031

Purpose

Expresses full length sfCherry2 and TagBFP fusion in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry+full length_TagBFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

3 mutations in sfCherry full length: E118Q, T128I…

Promoter

SFFV

Available since

Jan. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pDiGc

Plasmid

#59322

Purpose

Inducible expression of dsRed and constitutive expression of GFP in bacteria

Depositor

David Holden, Sophie Helaine

Article

Helaine et al Proc Natl Acad Sci U S A. 2010 Feb 23;10

Inserts

EGFP
dsRed

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pbad and rpsM

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pCFD5-NS

Plasmid

#149546

Purpose

Expression of pegRNA(s) and sgRNA(s) under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.

Depositor

Norbert Perrimon

Article

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Type

Empty backbone

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

U6

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-PKAKTRClover

Plasmid

#59146

Purpose

ORF encoding PKA KTR mClover flanked by gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

PKA Kinase Translocation Reporter (PRKACA Mouse, Human)

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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p3E-P2A-mKate2 no-pA

Plasmid

#80828

Purpose

P2A followed by mKate2 without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-P2A-mKate2 no pA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pGWT35S-mCherry-NLS

Plasmid

#194049

Purpose

Transient expression of mCherry-NLS in plant cell (Nucleus)

Depositor

Yutaka Kodama

Article

Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

Insert

mCherry

Use

Tags

SV40 (Nuclear localization signal)

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pDiGi

Plasmid

#59323

Purpose

Inducible expression of dsRed and GFP in bacteria

Depositor

David Holden, Sophie Helaine

Article

Helaine et al Proc Natl Acad Sci U S A. 2010 Feb 23;10

Inserts

EGFP
dsRed

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pbad and Plac

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pICH41421

Plasmid

#50339

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, nos (A. tumefaciens)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr-GCaMP6s

Plasmid

#184712

Purpose

Calcium biosensor

Depositor

Michiyuki Matsuda

Article

Watabe et al Elife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

GCaMP6s

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRSCupSup35GFP

Plasmid

#1087

Depositor

Susan Lindquist

Article

Glover et al Cell 1997 May 30;89(5):811-9.

Insert

SUP35 (SUP35 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2005

Availability

Academic Institutions and Nonprofits only

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pSFFV_sfCherry2(11)_TagBFP

Plasmid

#82606

Purpose

Expresses sfCherry2(11) and TagBFP fusion in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(11)_TagBFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

changed Gly12 to Ala in sfCherry11+

Promoter

SFFV

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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pTns(Ptr)

Plasmid

#175576

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

PtrTnsABC

Use

Tags

Expression

Bacterial

Mutation

NO

Promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDonorCm(Ptr)

Plasmid

#175577

Purpose

DNA donor for transposition

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

Mini-Tn, Cm cassette

Use

Tags

Expression

Bacterial

Mutation

NO

Promoter

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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