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Showing: 11601 - 11650 of 148131 results

  2. pNHA_OROV_Gc

    Plasmid
    #208936
    Purpose
    Expresses N-terminally HA-tagged Oropouche orthobunyavirus Gc protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    Gc
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted 1579 bp from 5' end, deleted 277 bp …
    Promoter
    CMV
    Available since
    Jan. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    208936-plasmid-map-sequence-id-418962

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  5. pAAV-syn-DIO-axon-jGCaMP8f

    Plasmid
    #220531
    Purpose
    For Cre-dependent enriched expression of GCaMP8f in axons
    Depositor
    Lin Tian
    Insert
    Axon-DIO-GCaMP8f
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSynapsin
    Available since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    220531-plasmid-map-sequence-id-436147

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  8. pET-(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis

    Plasmid
    #62373
    Purpose
    Expression of (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis in bacterial cells
    Depositor
    David Liu
    Article
    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do
    Insert
    (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
    Use
    Tags
    (-30)dGFP, 6xHis tag, Cathepsin cleavage site, an…
    Expression
    Bacterial
    Mutation
    Y67S amino acid substitution in (-30)GFP renders …
    Promoter
    T7
    Available since
    June 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    62373-plasmid-map-sequence-id-263816

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  13. pDule-pCNF

    Plasmid
    #85494
    Purpose
    Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.
    Depositor
    Ryan Mehl
    Article
    Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62
    Insert
    para-cyanophenylalanine Mj synthetase
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32L L65V F108W Q109M D158G I159P
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85494-plasmid-map-sequence-id-169861

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  31. mCer-4aa-VAMP2

    Plasmid
    #53233
    Purpose
    Fluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET.
    Depositor
    Robert Zucker
    Article
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Insert
    VAMP2 (Vamp2 Rat)
    Use
    Tags
    4aa-linker and mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only

  35. FRP2371

    Plasmid
    #127577
    Purpose
    Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, His3MX selection marker
    Depositor
    Joerg Stelling
    Article
    Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1
    Insert
    PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
    Use
    Synthetic Biology; Shuttle vector
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    127577-plasmid-map-sequence-id-252230

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  37. pHAGE-GFP

    Plasmid
    #106281
    Purpose
    A version of the pHAGE vector with ZsGreen dsRed expressed separated by an IRES. Note that there is little-to-no expression of dsRed.
    Depositor
    Jay Shendure
    Article
    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460
    Insert
    CMV-GFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    106281-plasmid-map-sequence-id-212556

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  38. pFC334

    Plasmid
    #87846
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymes
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    argB
    yA specific sgRNA
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    A. nidulans gpdA promoter with Hammerhead ribozym…
    Available since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    87846-plasmid-map-sequence-id-174613

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  41. pAAV-hDlx-Flex-GFP-Fishell_6

    Plasmid
    #83895
    Purpose
    Cre recombinase-dependent GFP expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV8, and AAV9
    Insert
    eGFP
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hDlx
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    83895-plasmid-map-sequence-id-160320

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  50. pGL4.22-VEGF-HRE::dLUC

    Plasmid
    #128096
    Purpose
    Hypoxia response element real-time luciferase reporter
    Depositor
    Chi Van Dang
    Article
    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:
    Insert
    Hypoxia response element (x5) from VEGF promoter (VEGFA Human)
    Use
    Luciferase
    Tags
    drives expression of destabilized luciferase (2CP…
    Expression
    Mammalian
    Mutation
    Promoter
    inserted VEGF hypoxia response elements (HRE) (x5)
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    128096-plasmid-map-sequence-id-249669

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Showing: 11601 - 11650 of 148131 results