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Plasmid#115515PurposePlasmid driving expression of membrane bound KillerRed fluorophore in zebrafish motor neuronsDepositorInsertMLS-KillerRed
UseGateway cloningTagsMLSExpressionMutationPromoter-3mnx1Available sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {ChETA}on-WPRE
Plasmid#111386PurposeAAV expression of ChETA (hChR2 with E123T/H134R mutations) fused to HA tag driven by hSynapsin promoter in Cre-ON manner for optogenetic activationDepositorInsertChETA
UseAAVTagsHAExpressionMammalianMutationhumanized ChR2, E123T/H134RPromoterhSynapsinAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEY79
Plasmid#191074Purposeflp-12 fluorescent neural reporter driving nuclear T-Sapphire expression (refer to NeuroPAL paper for expression)DepositorInsertflp-12 (flp-12 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY62
Plasmid#191020Purposeacr-5 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertacr-5 (acr-5 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAM-CAGGS-FLEX-H2B-GFP-P2A-N2c(G)
Plasmid#73475PurposeEncodes the G protein to enable transsynaptic RAV infectionDepositorInsertH2B-GFP-P2A-N2c(G)
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCer-Vamp2(qv/fw)
Plasmid#53238PurposeMutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.DepositorInsertVAMP2 (Vamp2 Rat)
UseTags4aa-linker and mCeruleanExpressionMammalianMutationMutated Q76 to V; F77 to W.PromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEY68
Plasmid#191065Purposeceh-14 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInsertceh-14 (ceh-14 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
prNFL-Q333P-EGFP
Plasmid#132602PurposeExpresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E Q333P mutationDepositorInsertNefl (Nefl Rat)
UseTagsEGFPExpressionMammalianMutation998A>C, 999G>C; Gln333ProPromoterCMVAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSC-ASCL1-IRES-GFP-T2A-Sox11
Plasmid#182233PurposeTo convert human skin fibroblasts into induced basal forebrain cholinergic neurons (hiBFCNs) in combination with LHX8, GBX1, FGF2 and two small molecules, forskolin and LDN-193189DepositorInsertASCL1-IRES-GFP-T2A-Sox11
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY64
Plasmid#191061Purposeaqp-6 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInsertaqp-6 (aqp-6 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
phNFL-E396K
Plasmid#132595PurposeExpresses human NFL containing the Charcot-Marie-Tooth disease type 2E E396K mutationDepositorInsertNEFL (NEFL Human)
UseTagsExpressionMammalianMutation1186G>A; Glu396LysPromoterCMVAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSC-ASCL1-IRES-Sox11
Plasmid#182234PurposeTo convert human skin fibroblasts into induced basal forebrain cholinergic neurons (hiBFCNs) in combination with LHX8, GBX1, FGF2 and two small molecules, forskolin and LDN-193189DepositorInsertASCL1-IRES-Sox11
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
phNFL-P440L
Plasmid#132596PurposeExpresses human NFL containing the Charcot-Marie-Tooth disease type 2E P440L mutationDepositorInsertNEFL (NEFL Human)
UseTagsExpressionMammalianMutation1319C>T; Pro440LeuPromoterCMVAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Ptn in situ probe
Plasmid#45632DepositorInsertPtn in situ probe (Ptn Mouse)
UseIn situTagsExpressionMutationfragment contains bp# 1241-1830 of NM_008973PromoterAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
prNFL-L269P-EGFP
Plasmid#132601PurposeExpresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E L269P mutationDepositorInsertNefl (Nefl Rat)
UseTagsEGFPExpressionMammalianMutation806T>C, 807G>C; Leu269ProPromoterCMVAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
prNFL-P441L-EGFP
Plasmid#132604PurposeExpresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E P441L mutationDepositorInsertNefl (Nefl Rat)
UseTagsEGFPExpressionMammalianMutation1322C>T, 1323A>G; Pro441LeuPromoterCMVAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
Non-targeting CRISPRi plasmid
Plasmid#154891PurposeHuman non-targeting CRISPRi gRNADepositorInsertNontargeting gRNA
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Flex-rev-GFPFANACfullWT
Plasmid#126551PurposeAAV Flex reverse plasmid with the coding sequence of eGFP fused to the N-term of the FMRFamide gated Na channelDepositorInserteGFP N-terminal fused to FMRFamide gate sodium channel
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
phNFL-N98S
Plasmid#132592PurposeExpresses human NFL containing the Charcot-Marie-Tooth disease type 2E N98S mutationDepositorInsertNEFL (NEFL Human)
UseTagsExpressionMammalianMutation293A>G; Asn98SerPromoterCMVAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Nectin-3 in situ probe
Plasmid#45633DepositorInsertNectin-3 in situ probe (Nectin3 Mouse)
UseIn situTagsExpressionMutationfragment contains bp# 1626-2039 of NM_021495PromoterAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only