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Plasmid#136455PurposeMammalian expression plasmid for c-myc-tagged PDGFRBDepositorInsertPDGFRbeta (PDGFRB Human)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-64: FUS-mEGFP
Plasmid#114410PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human FUSDepositorInsertFUS Homology Arms with linker-mEGFP (FUS Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceSept. 5, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
p3015b
Plasmid#209317PurposeExpresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.DepositorInsertGroup B Streptococcus-compatible single guide RNA
UseTagsExpressionBacterialMutationPromoterXyl/tet promoter that is constitutively activeAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-25:MAP1LC3B-mEGFP
Plasmid#101783PurposeHomology arms and mEGFP-linker sequence for N-terminus tagging of human MAP1LC3BDepositorInsertMAP1LC3B Homology Arms with mEGFP-linker (MAP1LC3B Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceOct. 31, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKan-PCUP1-9myc-AID*(N)
Plasmid#99527PurposeN-terminal 9myc-AID* degron cassette with CUP1 promoter and KanR marker for S. cerevisiaeDepositorInsertIAA17(71-117) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTags9xMycExpressionMutationPromoterCUP1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-trGluc
Plasmid#158959PurposeDNA donor plasmid containing repair template for Gaussia luciferase (Gluc) of bioluminescent repair reporter (BLRR).DepositorInserttrGluc
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV_Actb HR donor
Plasmid#97317PurposeHR donor for fusing a p2A-mCherry reporter to mouse Actb.DepositorInsertActb HR donor
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shLacZ-1735
Plasmid#115454PurposeConstitutive lentiviral expressionDepositorInsertshLacZ-1735
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX317-hcRED
Plasmid#115440PurposeConstitutive lentiviral expressionDepositorInserthcRED
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHDR_NANOS3-T2A-mVenus-PuroTK
Plasmid#222903PurposeHomology directed repair template for knocking in mVenus reporter to NANOS3.DepositorInsertmVenus
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKan-PCUP1-AID*(N)-9myc
Plasmid#99526PurposeN-terminal AID*-9myc degron cassette with CUP1 promoter and KanR marker for S. cerevisiaeDepositorInsertIAA17(71-116) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTags9xMycExpressionMutationPromoterCUP1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-43: GJA1-mEGFP
Plasmid#109120PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human GJA1DepositorInsertGJA1 Homology Arms with linker-mEGFP (GJA1 Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceMay 4, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXD71Cas10.9RT2-Pct5.1-crRNA(cadR)-RT(ΔcadR)
Plasmid#191647PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cadR-targeting spacer) cadR-deleting repair template,used for cadR deletionDepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(-)-GAPDH-IRES-EGFP
Plasmid#115918Purposedonor to insert IRES-EGFP at the GAPDH locus in human cellsDepositorInsertIRES-EGFP
UseDonor templateTagsdTomatoExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHyg-AID*
Plasmid#99515PurposeC-terminal AID* degron cassette with HygR marker for S. cerevisiaeDepositorInsertIAA17(71-114) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTagsExpressionMutationinternal inversion of nucleotides 265-312 of the …PromoterAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEasyG1_hph
Plasmid#184912PurposeTemplate to generate via PCR a single gRNA for expression in S. cerevisiaeDepositorInsertgRNA scaffold
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Halotag-loxP-P2A-dTKneo-pA-loxP
Plasmid#139485PurposePCR template for Halo-Tag gene knock-in donor vectorDepositorInsertHaloTag-loxP-P2A-dTKneo-pA-loxP
UseTagsHaloTagExpressionMammalianMutationPromoterCAG promoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(-)-ACTB-mEGFP
Plasmid#115917Purposedonor to insert EGFP at the ACTB locus in human cellsDepositorInsertACTB-mEGFP
UseDonor templateTagsEGFPExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.1RT1-Pct5.1-crRNA(dadR)-RT(ΔdadR)
Plasmid#191635PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer) dadR-deleting repair template, used for dadR deletionDepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_His-ZZ-TEV-Lis1
Plasmid#132539PurposePlasmid for insect cell expression of human full-length LIS1 with an N-terminal His-ZZ-LTLT tagDepositorInsertLis1 (PAFAH1B1 Human)
UseTags8x His, Linker-TEV-linker_TEV, and ZZExpressionInsectMutationPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only