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Showing: 4621 - 4640 of 7239 results
  1. pCasSA

    Plasmid
    #98211
    Purpose
    CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 19, 2017
    Availability
    Academic Institutions and Nonprofits only
  2. pEVOL-pBpF

    Plasmid
    #31190
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli in response to the amber codon, TAG.
    Depositor
    Insert
    M.j. p-benzoylphenylalanine RS (2 copies +tRNA)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32G, E107P, D158T, I159S
    Promoter
    Available since
    Dec. 9, 2011
    Availability
    Academic Institutions and Nonprofits only
  3. pTC-ApoE-Tet

    Plasmid
    #85578
    Purpose
    Sleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent transgene/shRNA expression (ApoE.HCR.hAAT promoter ) in mouse liver
    Depositor
    Insert
    CreER
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    ApoE.HCR.hAAT
    Available since
    Feb. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
  4. pCBC-DT1T2

    Plasmid
    #50590
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Insert
    T1, U626t plus, U629p, T2
    Use
    CRISPR; Pcr template
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
  5. pTara:500

    Plasmid
    #60717
    Purpose
    Expresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORF
    Depositor
    Insert
    T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
  6. pMSR0

    Plasmid
    #161984
    Purpose
    Allele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile ribotype 027 and other ribotypes. Uses inducible expression of CD2517.1 toxin as a counter-selection marker
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pLR16-Phes*

    Plasmid
    #98783
    Purpose
    Suicide vector for allelic exchange in Listeria utilizing an engineered counterselection marker for Listeria monocytogenes based on a mutated phenylalanyl-tRNA synthetase gene (pheS*).
    Depositor
    Insert
    pheS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    A309G
    Promoter
    Available since
    Jan. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  8. pJRA109

    Plasmid
    #209342
    Purpose
    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mTagBFP2 fusion protein.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. pJRA104

    Plasmid
    #209337
    Purpose
    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal HaloTag fusion protein.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  10. pDestTol2CG2-U6:gRNA

    Plasmid
    #63156
    Purpose
    Destination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR; Tol2 destination vector for gateway
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 13, 2015
    Availability
    Academic Institutions and Nonprofits only
  11. pCBC-DT2T3

    Plasmid
    #50591
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Insert
    T2, gRNA scaffold, U6-29t plus, U6-1p, T3
    Use
    CRISPR; Pcr template
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
  12. pUAP4

    Plasmid
    #136079
    Purpose
    Acceptor plasmid for L0 parts. Clone L0 parts into pUAP4 with SapI, lacZ blue-white screening. Release parts to assemble transcription units with BsaI.
    Depositor
    Insert

    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Nov. 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 4621 - 4640 of 7239 results