-
Plasmid#66858PurposeGenerate transgenic C.elegans with cYAP1- fused to GFP, in which Serine 104 is mutated to alanineDepositorInsertYAP-1 (yap-1 Nematode)
UseTagsGFPExpressionWormMutationSerine104 is mutated to alaninePromoterAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
Neo1.c-AP-His
Plasmid#71965PurposeExpresses the extracellular region of the Neogenin 1, isoform c protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertNeo1.c (Neo1 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Plxnb2(L)-AP-His
Plasmid#72002PurposeExpresses the extracellular region of the PlexinB2 protein (ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertPlxnb2 (Plxnb2 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
Plxnc1-AP-His
Plasmid#72005PurposeExpresses the extracellular region of the PlexinC1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertPlxnc1 (Plxnc1 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Unc5c.z-Fc-His
Plasmid#72182PurposeExpresses the extracellular region of the Unc5C, isoform z protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertUnc5c.z (Unc5c Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sdk2-AP-His
Plasmid#72009PurposeExpresses the extracellular region of the Sdk2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSdk2 (Sdk2 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Plxnb3-AP-His
Plasmid#72004PurposeExpresses the extracellular region of the PlexinB3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertPlxnb3 (Plxnb3 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
Unc5d.z-Fc-His
Plasmid#72185PurposeExpresses the extracellular region of the Unc5D, isoform z protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertUnc5d.z (Unc5d Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE Gal4 (1-94) HSF ABC Flag wt
Plasmid#1945DepositorInsertHeat Shock Factor 1 (HSF1 Human)
UseRetroviralTagsGal4 DBD and flagExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBABE Gal4(1-94) HSF ABC Flag ph
Plasmid#1947DepositorInsertHeat Shock Factor 1 (HSF1 Human)
UseRetroviralTagsGal4 DBD and flagExpressionMammalianMutationphenylalanine mutant (F418, F492, F500 A)PromoterAvailable sinceMarch 23, 2006AvailabilityAcademic Institutions and Nonprofits only -
EGFP-INPP5E
Plasmid#223564PurposeExpresses the full-length mouse INPP5E (Pharbin)DepositorInsertINPP5E (Inpp5e Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1302-AAV-EFSNC-dCjCas9-KRAB
Plasmid#223147PurposeExpression of KRAB with dCjCas9 and empty gRNA scaffoldDepositorInsertKRAB
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1312-AAV-EFSNC-dSaCas9-KRAB
Plasmid#223157PurposeExpression of KRAB with dSaCas9 and empty gRNA scaffoldDepositorInsertKRAB
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK2043-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9
Plasmid#223163Purpose2nd gen. AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA ScaffoldDepositorTypeEmpty backboneUseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterAvailable sinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLCHKOv3
Plasmid#209025PurposeLentiviral backbone for expressing U6 driven hybrid guide (hg)RNAs with BveI cloning sites and puromycin selection markerDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLCHKOv3-mClover3
Plasmid#209034PurposeLentiviral backbone for expressing U6 driven hybrid guide (hg)RNAs with BveI cloning sites, mClover3 and puromycin selection markerDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLCHKOv3-mCherry
Plasmid#209035PurposeLentiviral backbone for expressing U6 driven hybrid guide (hg)RNAs with BveI cloning sites, mCherry and puromycin selection markerDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-Nova1-GFP
Plasmid#217033PurposeOverexpression of Nova in DRG neurons promotes the "mature" splicing pattern observed in CNS neuronsDepositorInsertNova1 (Nova1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-IgK-pHluorin-TM-mRuby
Plasmid#185546PurposeExpresses surface-localized pHluorin fused to intracellular expression of mRuby under CMV promoterDepositorInsertIgK-pHluorin-TM-mRuby
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-2xflox-BRX-eGFP-NLS
Plasmid#217535PurposeAAV vector to drive the expression of eGFP in genetically defined cell populations under the control of the 4xBRE regulatory element of SMAD1 transcription factor and miniXon splicing casette in vivoDepositorArticleInsert4X BRE reporter and miniXon casette (Smad1 Mouse)
UseAAVTagsExpressionMammalianMutationPromoterBMP response element (BRE)Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_T3_ABE8e_IVT
Plasmid#201676PurposePlasmid to be used as DNA template for in vitro RNA transcription of the ABE8e base editor (A to G) by T3 RNA polymerase. The plasmid contains optimised 5'UTR and 3'UTR to improve protein expression.DepositorInsertecTadA(8e)-nSpCas9
UseCRISPR; Vector for in-vitro transcriptionTagsExpressionMutationPromoterT3 promoterAvailable sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHDR-USR-SpCas9
Plasmid#208829PurposeHDR Universal Surrogate Reporter with a sgRNA and consistently expressed SpCas9. The PuroR reporter gene was designed to be repaired as a selecting marker gene only by the sgRNA/Cas9-triggered HDR.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAIP-hXRCC1pd-eGFP
Plasmid#206033PurposeMammalian expression of PAR-deficient hXRCC1pd coupled to eGFP under the control of a CAG promoterDepositorInserthXRCC1
UseTagseGFPExpressionMammalianMutationS103A,R186A,S184A,S193A,S219A,S220A,S236A,S268APromoterCAGAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET30b jGCaMP8f L27A
Plasmid#204138PurposeProduction of recombinant calcium sensor jGCaMP8f L27A fast decay variant in E.coliDepositorInsertjGCaMP8f L27A
UseTagsN-terminal His tagExpressionBacterialMutationLeucine 27 changed to AlaninePromoterT7Available sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP8f L27A
Plasmid#204140PurposeFluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with L27A mutationDepositorInsertjGCaMP8f L27A
UseTagsN-terminal His tagExpressionMammalianMutationLeucine 27 changed to AlaninePromoterCMVAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBa.HALO-KIF3A tail 419-702
Plasmid#193924PurposeMammalian expression of Halo-tagged Kif 3A tail 419-702DepositorInsertKif 3A (KIF3A Human)
UseTagsHaloExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lnig-INTR-GFP
Plasmid#208904Purposeencodes the inotocin receptor (oxytocin/vasopressin-like) from the ant Lasius niger, tagged with GFPDepositorInsertINTR
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
EYFP Cav2.2 pRK5
Plasmid#206099Purposeexpression of rabbit Cav2.2 calcium channel with a N-terminal EYFP tag and an exofacial double HA tag in domain IIDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-IEo
Plasmid#201992PurposeAAV vector mediating inducible expression of codon optimized immediate early gene IE180 of pseudorabies virusDepositorInsertInducible expression of codon optimized immediate early gene IE180 of pseudorabies virus
UseAAVTagsExpressionMutationPromoterTight TREAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SFFV-mCh-GATA2-R398W
Plasmid#193084Purposeconstitutive expression of mCherry fused to human Gata2 with mutation R398W in mammalian cellsDepositorInsertmCh-GATA2-R398W
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-Gata2-C373R
Plasmid#193085Purposeconstitutive expression of mouse Gata2 with mutation C373R in mammalian cellsDepositorInsertGata2-C373R
UseLentiviralTagsExpressionMammalianMutationPromoterUbcAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-Gata2-L359V
Plasmid#193086Purposeconstitutive expression of mouse Gata2 with mutation L359V in mammalian cellsDepositorInsertGata2-L359V
UseLentiviralTagsExpressionMammalianMutationPromoterUbcAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-MD-Gata2
Plasmid#193090Purposeconstitutive expression of the mitosis degradation (MD) domain of cyclin B1 fused to mouse Gata2 in mammalian cellsDepositorInsertMD-Gata2
UseLentiviralTagsExpressionMammalianMutationPromoterUbcAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-MDmut-mTurquoise
Plasmid#193095Purposeconstitutive expression of a mutated (mut, R42A), non-functional version of the mitosis degradation (MD) domain of cyclin B1 fused to mTurquoise in mammalian cellsDepositorInsertMDmut-mTurquoise
UseLentiviralTagsExpressionMammalianMutationPromoterUbcAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
SFFV-mCh-GATA2
Plasmid#193071Purposeconstitutive expression of mCherry fused to human Gata2 in mammalian cellsDepositorInsertmCh-GATA2
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
SFFV-mCh-GATA2-R293Q
Plasmid#193072Purposeconstitutive expression of mCherry fused to human Gata2 with mutation R293Q in mammalian cellsDepositorInsertmCh-GATA2-R293Q
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
SFFV-mCh-GATA2-P304H
Plasmid#193073Purposeconstitutive expression of mCherry fused to human Gata2 with mutation P304H in mammalian cellsDepositorInsertmCh-GATA2-P304H
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
SFFV-mCh-GATA2-R307W
Plasmid#193074Purposeconstitutive expression of mCherry fused to human Gata2 with mutation R307W in mammalian cellsDepositorInsertmCh-GATA2-R307W
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
SFFV-mCh-GATA2-A318T
Plasmid#193075Purposeconstitutive expression of mCherry fused to human Gata2 with mutation A318T in mammalian cellsDepositorInsertmCh-GATA2-A318T
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
SFFV-mCh-GATA2-L321F
Plasmid#193076Purposeconstitutive expression of mCherry fused to human Gata2 with mutation L321F in mammalian cellsDepositorInsertmCh-GATA2-L321F
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
SFFV-mCh-GATA2-R330Q
Plasmid#193077Purposeconstitutive expression of mCherry fused to human Gata2 with mutation R330Q in mammalian cellsDepositorInsertmCh-GATA2-R330Q
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
SFFV-mCh-GATA2-T354M
Plasmid#193078Purposeconstitutive expression of mCherry fused to human Gata2 with mutation T354M in mammalian cellsDepositorInsertmCh-GATA2-T354M
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
SFFV-mCh-GATA2-L359V
Plasmid#193079Purposeconstitutive expression of mCherry fused to human Gata2 with mutation L359V in mammalian cellsDepositorInsertmCh-GATA2-L359V
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
SFFV-mCh-GATA2-R361L
Plasmid#193080Purposeconstitutive expression of mCherry fused to human Gata2 with mutation R361L in mammalian cellsDepositorInsertmCh-GATA2-R361L
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
SFFV-mCh-GATA2-R362Q
Plasmid#193081Purposeconstitutive expression of mCherry fused to human Gata2 with mutation R362Q in mammalian cellsDepositorInsertmCh-GATA2-R362Q
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
SFFV-mCh-GATA2-C373R
Plasmid#193082Purposeconstitutive expression of mCherry fused to human Gata2 with mutation C373R in mammalian cellsDepositorInsertmCh-GATA2-C373R
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
SFFV-mCh-GATA2-R396Q
Plasmid#193083Purposeconstitutive expression of mCherry fused to human Gata2 with mutation R396Q in mammalian cellsDepositorInsertmCh-GATA2-R396Q
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFUW-tetO-mCh-Gata2
Plasmid#193061Purposedoxycycline-inducible expression of mCherry fused to mouse Gata2 in mammalian cellDepositorInsertmCh-Gata2
UseLentiviralTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFUW-tetO-mCh-Gfi1b
Plasmid#193062Purposedoxycycline-inducible expression of mCherry fused to mouse Gfi1b in mammalian cellsDepositorInsertmCh-Gfi1b
UseLentiviralTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFUW-teto-mch-cFos
Plasmid#193063Purposedoxycycline-inducible expression of mCherry fused to mouse cFos in mammalian cellDepositorInsertmch-cFos
UseLentiviralTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only