Addgene

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pENTR-SYNZIP21

Plasmid

#80675

Purpose

Encodes the synthetic coiled coil peptide SYNZIP21

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP21

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-SYNZIP18

Plasmid

#80672

Purpose

Encodes the synthetic coiled coil peptide SYNZIP18

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP18

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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pRD134

Plasmid

#132378

Purpose

contains the sequence of mScarlet for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

mScarlet

Use

Greengate entry plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pYTK001_E2Ab

Plasmid

#183082

Purpose

2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use in bi-cistronic and as the first 2A peptide in tri-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

E2A

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pGD165

Plasmid

#132370

Purpose

contains the sequence of eGFP for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

linker-eGFP

Use

Greengate entry plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pRD53

Plasmid

#132377

Purpose

contains the sequence of mCherry for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

mCherry

Use

Greengate entry plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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C121-E12: NanoLuc

Plasmid

#162891

Purpose

Standard Gateway entry clone encoding NanoLuc luciferase

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Nanoluciferase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pGD255

Plasmid

#132376

Purpose

contains the sequence of Venus for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

linker-Venus

Use

Greengate entry plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pPTK017-3b-yEGFP_Sec

Plasmid

#84976

Purpose

Type 3b, Green fluorescent protein

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Green fluorescent protein

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Non

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pEnt R3L2 TetO(fl)-3

Plasmid

#27107

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

TetO promoter

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

March 16, 2011

Availability

Academic Institutions and Nonprofits only

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pYPQ138A

Plasmid

#69280

Purpose

Golden Gate entry vector; 8th gRNA under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ137A

Plasmid

#69279

Purpose

Golden Gate entry vector;7th gRNA under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pEnt R3L2-MCS

Plasmid

#26799

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

multiple cloning sequence

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pENTR-SYNZIP16

Plasmid

#80670

Purpose

Encodes the synthetic coiled coil peptide SYNZIP16

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP16

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-SYNZIP12

Plasmid

#80667

Purpose

Encodes the synthetic coiled coil peptide SYNZIP12

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP12

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-SYNZIP13

Plasmid

#80668

Purpose

Encodes the synthetic coiled coil peptide SYNZIP13

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP13

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-SYNZIP14

Plasmid

#80669

Purpose

Encodes the synthetic coiled coil peptide SYNZIP14

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP14

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-SYNZIP10

Plasmid

#80665

Purpose

Encodes the synthetic coiled coil peptide SYNZIP10

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP10

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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R777-E006 Hs.AKT3-nostop

Plasmid

#70290

Purpose

Gateway ORF clone of human AKT3 [NM_005465.4] without stop codon (for C-terminal fusions)

Depositor

Dominic Esposito

Insert

AKT 3 (AKT3 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pGD253

Plasmid

#132374

Purpose

contains the sequence of mRuby2 for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

mRuby2

Use

Greengate entry plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge