Addgene

We narrowed to 15,711 results for: puromycin

Showing: 4601 - 4620 of 15711 results

Results per page:

Sort results by:

SEP-Nanoluc-cyOFP1

Plasmid

#141082

Purpose

prototype of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

SEP-Nanoluc-cyOFP1

Use

Lentiviral

Tags

Expression

Mutation

Deletion of amino acids DISGG from positions 673 …

Promoter

EF1α

Available since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX302 IL10RA-V5 puro

Plasmid

#47552

Purpose

Lentiviral expression of V5-tagged IL10RA in mammalian cells

Depositor

Kevin Janes

Article

Kang et al Sci Signal. 2013 Aug 6;6(287):rs13. doi:

Insert

IL10RA (IL10RA Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
PlmdCas12e KRAB

Plasmid

#188506

Purpose

Expresses FLAG tagged PlmdCas12e KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12e

Use

CRISPR and Lentiviral

Tags

NLS-FLAG-KRAB-NLS

Expression

Mammalian

Mutation

D659A/E756A/D922A

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pIND-CLucZ

Plasmid

#53224

Purpose

Inducible expression of Cypridina luciferase and shRNAmir of interest

Depositor

Thorsten Stiewe

Article

Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1

Inserts

Cypridina luciferase
non-silencing shRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pMRXIP-Myc-SNAP29

Plasmid

#116944

Purpose

Expressing Myc-SNAP29 for mammalian cells

Depositor

Noboru Mizushima

Article

Matsui et al J Cell Biol. 2018 Aug 6;217(8):2633-2645

Insert

synaptosomal-associated protein, 29kDa (SNAP29 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLib6.4-Aalb_744

Plasmid

#176663

Purpose

Expression of sgRNA under Ae. albopictus U6 (AALF029744) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GWF180

Plasmid

#133607

Purpose

CAG-DNCR2-VPR-IRES-Puro-WPRE-SV40PA-PGK-NS3aH1-tagBFP-SpdCas9

Depositor

Dustin Maly

Article

Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

Insert

DNCR2-VPR, NS3aH1-tagBFP-SpdCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pM378

Plasmid

#173174

Purpose

Expresses C9-1 gRNA-12xMBS from hU6 promoter

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

C9-1 targeting gRNA-12xMBS

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
sgTrack-Gateway

Plasmid

#114011

Purpose

3rd generation lentiviral empty sgRNA expression vector with a Gateway cassette for co-expression of a reporter cDNA

Depositor

Charles Rudin

Article

Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRX-IP/Venus-mDFCP1

Plasmid

#58742

Purpose

Expresses Venus-mDFCP1 in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

ZFYVE1 zinc finger, FYVE domain containing 1 (Zfyve1 Mouse)

Use

Retroviral

Tags

Venus

Expression

Mutation

Promoter

Available since

Dec. 8, 2014

Availability

Academic Institutions and Nonprofits only
pCSIIpuro-4493NES

Plasmid

#175811

Purpose

The lentiviral vector for Booster-PKA, a Red-Shifted genetically encoded FRET biosensor for monitoring PKA activity.

Depositor

Michiyuki Matsuda

Article

Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

Insert

Booster-PKA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SMC3 A8.4 gRNA

Plasmid

#90897

Purpose

3rd generation lentiviral gRNA plasmid targeting human SMC3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SMC3 (Guide Designation A8.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
SMC3 A7.4 gRNA

Plasmid

#90896

Purpose

3rd generation lentiviral gRNA plasmid targeting human SMC3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SMC3 (Guide Designation A7.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
SMC3 H8.1 gRNA

Plasmid

#90895

Purpose

3rd generation lentiviral gRNA plasmid targeting human SMC3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SMC3 (Guide Designation H8.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
SMC3 H7.1 gRNA

Plasmid

#90894

Purpose

3rd generation lentiviral gRNA plasmid targeting human SMC3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SMC3 (Guide Designation H7.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMH-SFB-RNF169

Plasmid

#99392

Purpose

Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged RNF169

Depositor

Michael Huen

Article

Chen et al J Biol Chem. 2012 Aug 10;287(33):27715-2

Insert

RNF169 (RNF169 Human)

Use

Tags

SFB (S protein-FLAG-Streptavidin binding peptide)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PLK1 G6.1 gRNA

Plasmid

#90835

Purpose

3rd generation lentiviral gRNA plasmid targeting human PLK1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PLK1 (Guide Designation G6.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR-sgUFSP2

Plasmid

#86134

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting UFSP2

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

sgRNA targeting UFSP2 (UFSP2 )

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 15, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
plko.1/shMCT4 477

Plasmid

#99598

Purpose

Knockdown of MCT4 expression

Depositor

Eli Bar

Article

Lim et al Oncogene. 2014 Aug 28;33(35):4433-41. do

Insert

MCT4 (SLC16A3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRetro-XT-PAK2-CFP(puro)-L107F

Plasmid

#81180

Purpose

Retroviral vector for expression of kinase active PAK2 with a CFP fusion

Depositor

Angeliki Malliri

Article

Whalley et al Nat Commun. 2015 Jun 16;6:7437. doi: 10.

Insert

p21 (RAC1) activated kinase 2 (PAK2 Human)

Use

Retroviral

Tags

CFP

Expression

Mutation

L107F

Promoter

P-tight TRE

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only