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Addgene

pLX302 IL10RA-V5 puro
(Plasmid #47552)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47552 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLX302
  • Backbone manufacturer
    Addgene #25896
  • Backbone size w/o insert (bp) 9573
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    IL10RA
  • Alt name
    CD210
  • Alt name
    HIL-10R
  • Alt name
    CD210a
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1737
  • Mutation
    None
  • GenBank ID
    NM_001558
  • Entrez Gene
    IL10RA (a.k.a. CD210, CD210a, CDW210A, HIL-10R, IL-10R1, IL10R)
  • Promoter CMV
  • Tag / Fusion Protein
    • V5 (C terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Thermo Open Biosystems Human ORFeome version 5.1 #9496

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLX302 IL10RA-V5 puro was a gift from Kevin Janes (Addgene plasmid # 47552 ; http://n2t.net/addgene:47552 ; RRID:Addgene_47552)
  • For your References section:

    Simultaneous profiling of 194 distinct receptor transcripts in human cells. Kang BH, Jensen KJ, Hatch JA, Janes KA. Sci Signal. 2013 Aug 6;6(287):rs13. doi: 10.1126/scisignal.2003624. 10.1126/scisignal.2003624 PubMed 23921087