pLX302 IL10RA-V5 puro
(Plasmid
#47552)
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PurposeLentiviral expression of V5-tagged IL10RA in mammalian cells
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 47552 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLX302
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Backbone manufacturerAddgene #25896
- Backbone size w/o insert (bp) 9573
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameIL10RA
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Alt nameCD210
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Alt nameHIL-10R
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Alt nameCD210a
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SpeciesH. sapiens (human)
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Insert Size (bp)1737
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MutationNone
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GenBank IDNM_001558
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Entrez GeneIL10RA (a.k.a. CD210, CD210a, CDW210A, HIL-10R, IL-10R1, IL10R)
- Promoter CMV
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Tag
/ Fusion Protein
- V5 (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThermo Open Biosystems Human ORFeome version 5.1 #9496
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLX302 IL10RA-V5 puro was a gift from Kevin Janes (Addgene plasmid # 47552 ; http://n2t.net/addgene:47552 ; RRID:Addgene_47552) -
For your References section:
Simultaneous profiling of 194 distinct receptor transcripts in human cells. Kang BH, Jensen KJ, Hatch JA, Janes KA. Sci Signal. 2013 Aug 6;6(287):rs13. doi: 10.1126/scisignal.2003624. 10.1126/scisignal.2003624 PubMed 23921087