Showing: 4601 - 4620 of 4794 results
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Plasmid#214728PurposeIn mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses pang17, RmYN02, Rf1, and WIV1 each separated by GS-linkers with an N-terminal SpyTag fusionDepositorInsertSpyTag-Alternate Quartet
UseTagsSpyTagExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
SpyTag-Quartet [SARS1]
Plasmid#214729PurposeIn mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV each separated by GS-linkers with an N-terminal SpyTag fusionDepositorInsertSpyTag-Quartet [SARS1] (S Synthetic)
UseTagsSpyTagExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
SpyTag-Kraken Quartet
Plasmid#214730PurposeIn mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 XBB.1.5 separated by GS-linkers with an N-terminal SpyTagDepositorInsertSpyTag-Kraken Quartet (S Synthetic)
UseTagsSpyTagExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
SpyTag-Quartet_NoLinker
Plasmid#214731PurposeIn mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 with no linkers between the RBDs and an N-terminal SpyTagDepositorInsertSpyTag-Quartet_NoLinker (S Synthetic)
UseTagsSpyTagExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core
Plasmid#61357Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (D1399Y)
Plasmid#61358Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; D1399Y mutation i…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (C1204R)
Plasmid#61361Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation C1204R) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; C1204R mutation i…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (Y1467F)
Plasmid#61362Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; Y1467F mutation i…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA TMEM 138-flag
Plasmid#41628DepositorInsertTransmembrane Protein 138 (TMEM138 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
TMEM138-mCherry
Plasmid#41630DepositorInsertTransmembrane Protein 138 (TMEM138 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
TMEM216-mCherry
Plasmid#41633DepositorInsertTransmembrane Protein 216 (TMEM216 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090
Plasmid#103871PurposedPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to selectively edit adenosine to inosine in RNA when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAVDepositorInsertsUseCRISPRTagsGS and HIV NESExpressionMammalianMutationChanged histidne 133 to alanine and histidine 105…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mPC1_4119-eGFP
Plasmid#108394PurposeGenomic region, including intron 45, to express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293DepositorInsertPKD1 (PKD1 Mouse)
UseTagseGFPExpressionMammalianMutationvariant ID rs13468401 missense variant R4237QPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CD16.7-hPC1-eGFP-3HA
Plasmid#108401PurposeExpress cytoplasmic tail human PC1 from amino acid 4077 to 4302 with a membrane anchorDepositorInsertPKD1 (PKD1 Human)
UseTagseGFP-3HAExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-hPC1_MTS_L4137P-eGFP
Plasmid#108403PurposeExpress putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely pathogenic point mutation.DepositorInsertPKD1 (PKD1 Mouse)
UseTags3xHA and eGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-hPC1_MTS_L4139P-eGFP-3HA
Plasmid#108404PurposeExpress putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely pathogenic point mutation.DepositorInsertPKD1 (PKD1 Human)
UseTags3xHA and eGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-hPC1_MTS_V4146I-eGFP
Plasmid#108405PurposeExpress putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely non-pathogenic variant.DepositorInsertPKD1 (PKD1 Mouse)
UseTags3xHA and eGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
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S10A mutant of H3S10ph biosensor
Plasmid#120810PurposeFRET biosensor. Eliminating the H3 Serine 10 phosphorylation site in H3S10ph biosensor to abolish the detection capabilityDepositorInsertMouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa) S10A-YFP
UseTagsExpressionMammalianMutationSerine 10 is mutated to Alanine, and Threonine 3,…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-N1
Plasmid#125547PurposeGateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment1DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment1 attached to n-terminus of Gatew…ExpressionBacterial and MammalianMutationPromotersynthetic promoter containing yeast, mammalian, a…AvailabilityAcademic Institutions and Nonprofits only
Showing: 4601 - 4620 of 4794 results