Showing: 4601 - 4620 of 18079 results
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Plasmid#47032PurposeThis is a Gateway destination vector for secreted expression in Drosophila cell culture by induction via CuSO4. Inserts will be C-terminally tagged with Fc, V5 and hexahistidine tags.DepositorTypeEmpty backboneUseSecreted expression in drosophila cultureTagsFc(human IgG1), HRV 3C Protease Cleavage site, He…ExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)AvailabilityAcademic Institutions and Nonprofits only
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pJBEI-6409
Plasmid#47048PurposeBglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coliDepositorInsertCodon-optimized sequences for MEV pathway expression in E. coli to produce limonene
UseSynthetic Biology; BglbrickTagsExpressionBacterialMutationPromoterPlacUV5AvailabilityAcademic Institutions and Nonprofits only -
pJBEI-6410
Plasmid#47049PurposeBglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coliDepositorInsertCodon-optimized sequences for MEV pathway expression in E. coli to produce limonene
UseSynthetic Biology; BglbrickTagsExpressionBacterialMutationPromoterPlacUV5AvailabilityAcademic Institutions and Nonprofits only -
pJBEI-6411
Plasmid#47050PurposeBglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coliDepositorInsertahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase
UseSynthetic Biology; BglbrickTagsExpressionBacterialMutationPromoterPbadAvailabilityAcademic Institutions and Nonprofits only -
pECIA14
Plasmid#47051PurposeThis is a Gateway destination vector for secreted expression in Drosophila cell culture by CuSO4 induction. Inserts will be pentamerized and tagged with Alkaline phosphatase, FLAG and His tagsDepositorTypeEmpty backboneUseSecreted expression in drosophila cultureTagsAlkaline Phosphatase (human, placental), Flag, HR…ExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)AvailabilityAcademic Institutions and Nonprofits only -
pENTR-FOXP2
Plasmid#47053PurposeExpresses forkhead box P2DepositorInsertForkhead Box P2 (FOXP2 Human)
UseGateway entry vectorTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pREXC3GH
Plasmid#47077PurposeFX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and C-terminal 3C protease cleavage site, GFP and 10x His tagsDepositorTypeEmpty backboneUseLactococcus lactis expressionTags3C protease site, GFP, and HisExpressionMutationPromoterNIS (nisin)AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MAP3K5 (WT)
Plasmid#47104PurposeExpresses wild type human MAP3K5 in mammalian cellsDepositorInsertMAP3K5 (MAP3K5 Human)
UseTagsFLAGExpressionMammalianMutationNAPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9
Plasmid#47106PurposeExpresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cellsDepositorInsertdCas9
UseCRISPRTagsFlag and SV40 NLSExpressionMammalianMutationD10A, H840A (catalytically inactive)PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-VP64
Plasmid#47107PurposeExpresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cellsDepositorInsertdCas9-VP64
UseCRISPRTagsFlag, HA, SV40 NLS, and VP64ExpressionMammalianMutationD10A, H840A (catalytically inactive)PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pSPgRNA
Plasmid#47108PurposeExpresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cellsDepositorHas ServiceCloning Grade DNAInsertSPgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pBS-HA::UPRT
Plasmid#47110Purposefor subcloning UPRT into expression vectorsDepositorInserturacil phosphoribosyltransferase
UseTagsHAExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Cas9m2
Plasmid#47314PurposeCas9 D10A+H840ADepositorInsertCas9m2
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Cas9m4
Plasmid#47316PurposeCas9 D10A+D839A+H840A+N863ADepositorInsertCas9m4
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Cas9m4-VP64
Plasmid#47319PurposeCas9m4 ActivatorDepositorInsertCas9m4-VP64
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
reporter-gT1
Plasmid#47320PurposeTF reporter for gRNA-AAVS1_T1 (RNA-guided dTomato expression)DepositorInsertminCMV-dTomato_pA
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCS2-Cas9
Plasmid#47322PurposeFor in vivo expression of Cas9 or generation of Cas9 mRNADepositorInsertCas9
UseCRISPR; Xenopus and zebrafish expressionTagsExpressionMammalianMutationPromoterSP6AvailabilityAcademic Institutions and Nonprofits only -
FLAG-IFT57-pcDNA3
Plasmid#47326PurposeExpresses FLAG epitope tagged IFT57 in mammalian cellsDepositorInsertIFT57 (Ift57 Mouse)
UseTagsFLAG epitopeExpressionMammalianMutationPromotercmvAvailabilityAcademic Institutions and Nonprofits only -
pET-28b-Cas9-His
Plasmid#47327PurposeFor in vitro expression and purification of Cas9 proteinDepositorInsertCas9
UseCRISPRTagsHisExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBabe TIR1-9myc
Plasmid#47328PurposeFor retroviral expression of E3 ubiquitin ligase osTIR1 with N-term NES and C-term 9x myc tagDepositorInsertTRANSPORT INHIBITOR RESPONSE 1
UseRetroviralTags9x Myc and NES (nuclear export signal - ALQKKLEEL…ExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 4601 - 4620 of 18079 results