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pYPQ-NF-L2-dMb2Cas12a

Plasmid

#177684

Purpose

Gateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editing

Depositor

Yiping Qi

Article

Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

Insert

FokI-dMb2Cas12a

Use

CRISPR; Gateway compatible foki-dmb2cas12a entry …

Tags

NLS

Expression

Plant

Mutation

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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Pb mD3S4-dN

Plasmid

#180252

Purpose

P. berghei D3 (502-617) construct with N516T, S533N and N539Q mutations to abolish N-glycosylation was used for crystallization.

Depositor

Timothy Springer

Article

Feng et al Elife. 2021 Dec 23;10. pii: 74707. doi:

Insert

D3 of P. berghei HAP2 with N-glycosylation sites mutated

Use

Tags

6His

Expression

Mammalian

Mutation

N516T, S533N and N539Q

Promoter

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Rnt1-dsRBD(+6A)

Plasmid

#171552

Purpose

Encoding part of the budding yeast Rnt1 protein from L366 to S453

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Rnt1 (RNT1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

a six-alanine insertion in the L1 region

Promoter

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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PclpB riboswitch GFP pZa

Plasmid

#170078

Purpose

GFP expression under the control of E. coli clpB promoter engineered with riboregulators

Depositor

Urartu Seker

Article

Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

Insert

Green fluorescent protein

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PclpB

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Rnt1-dsRBD(+3A)

Plasmid

#171551

Purpose

Encoding part of the budding yeast Rnt1 protein from L366 to S453

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Rnt1 (RNT1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

a three-alanine insertion in the L1 region

Promoter

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Rnt1-dsRBD(+6)

Plasmid

#171550

Purpose

Encoding part of the budding yeast Rnt1 protein from L366 to S453

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Rnt1 (RNT1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

a six amino acid_TRLTEG_insertion in the L1 region

Promoter

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Rnt1-dsRBD

Plasmid

#171549

Purpose

Encoding part of the budding yeast Rnt1 protein from L366 to S453

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Rnt1 (RNT1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Xlrbpa

Plasmid

#171547

Purpose

Encoding part of the Xenopus Xlrbpa-2 protein from M120 to L202

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Xlrbpa (prkra.L Frog)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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RCP83

Plasmid

#163466

Purpose

Backbone plasmid for insertion of the Saccharomyces cerevisiae promoter library and yeGFP

Depositor

Frank Albert

Article

Renganaath et al Elife. 2020 Nov 12;9. pii: 62669. doi: 1

Inserts

Promoter fragment (Sharon2012_1922)
His3 minimal promoter
yeGFP

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pre_II_CS3_7746_v3

Plasmid

#126856

Purpose

7653 b scaffold from compatibility group 3, made by method II, containing 2 Zn-dependent self-cleaving DNAzyme cassette for lbisection into 2 3780 b scaffolds

Depositor

Hendrik Dietz

Article

Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

Insert

II_CS3_7746_v3

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pre_II_CS3_7653_v2

Plasmid

#126855

Purpose

7653 b scaffold from compatibility group 3, made by method II, containing 1 Zn-dependent self-cleaving DNAzyme cassette for linearisation into 7560 b scaffold

Depositor

Hendrik Dietz

Article

Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

Insert

II_CS3_7653_v2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pHIs8-4b-SaDAH::G226D

Plasmid

#140130

Purpose

Expresses SaDAH::G226D variant with N-terminal 8xHis-tag in BL21 E. coli

Depositor

Jing-Ke Weng

Article

Kim et al Nat Commun. 2020 Apr 20;11(1):1867. doi:

Insert

Dechloroacutumine halogenase G226D variant from Sinomenium acutum

Use

Tags

8x-His, TEV protease site (ENLYFQ)

Expression

Bacterial

Mutation

changed Gly226 to Asp226

Promoter

T7

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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tol2-lyzC-miR-100-1-dendra2

Plasmid

#105438

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

miR-100-1 Dendra2 (mir100-1 Zebrafish)

Use

Zebrafish

Tags

N/A

Expression

Mutation

Promoter

Available since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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pFB HTB_hMSH6_T1219D

Plasmid

#135466

Purpose

Expression human MSH6_T1219D mutant protein using baculovirus

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Insert

human MSH6_T1219D (MSH6 Human)

Use

Tags

His-tag

Expression

Insect

Mutation

changed threonine to aspartic acid

Promoter

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pTK-EdnrbE2-Cerulean

Plasmid

#127771

Purpose

Enhancer reporter construct containing the chicken EdnrB enhancer, E2 driving Cerulean activity

Depositor

Tatjana Sauka-Spengler

Article

Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

Insert

ednrbE2

Use

Enhancer reporter construct

Tags

Expression

Mutation

Promoter

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pTK-EdnrbE1-Citrine

Plasmid

#127773

Purpose

Enhancer reporter construct containing the chicken EdnrB enhancer E1 driving Citrine activity

Depositor

Tatjana Sauka-Spengler

Article

Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

Insert

ednrbE1

Use

Enhancer reporter construct

Tags

Expression

Mutation

Promoter

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-Sox3gRNA-T2

Plasmid

#127778

Purpose

Chicken sox3 gRNA target site 2, cloned into the pU6 sgRNA (backbone #92359)

Depositor

Tatjana Sauka-Spengler

Article

Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

Insert

sox3gRNAT2

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pLY133

Plasmid

#130961

Purpose

Engineered sgRNA-LEB4 generator including Anderson promoter J23100 for golden gate assembly

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEB4

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Anderson promoter J23100

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_Cerulean_Nanotag_92_positive_Control

Plasmid

#130573

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

FoxD3_cranial_neural_crest_enhancer_NC4

Use

Enhancer cloning nanotag reporter vector

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_Cerulean_Nanotag_91_positive_Control

Plasmid

#130572

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

FoxD3_cranial_neural_crest_enhancer_NC3

Use

Enhancer cloning nanotag reporter vector

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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