Addgene

We narrowed to 21,940 results for: Nov

Showing: 4581 - 4600 of 21940 results

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mc2 puro EF1a

Plasmid

#206221

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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mc2 blast

Plasmid

#206217

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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mc2 hygro

Plasmid

#206216

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pminiTol2-4kb wnt3 promoter-PMTmApple

Plasmid

#203794

Purpose

Tol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of PMTmApple for zebrafish transgenesis

Depositor

Thorsten Wohland

Article

Veerapathiran et al Elife. 2020 Nov 25;9:e59489. doi: 10.755

Insert

PMT

Use

Zebrafish expression

Tags

mApple

Expression

Mutation

Promoter

4kb Wnt3 promoter

Available since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pPANI2A2E2-Crimson

Plasmid

#193758

Purpose

Expresses E2-Crimson under the control of PANI2 synthetic promoter, ColE1 origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

E2-Crimson

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PANI2 synthetic promoter carrying binding sites f…

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpH-DmeIF4E_31-248_AD

Plasmid

#148517

Purpose

Bacterial Expression of DmeIF4E_31-248

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmeIF4E_31-248

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pET PI-SceI C-His K403A

Plasmid

#195612

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K403A active site mutation

Depositor

Frederick Gimble

Article

Gimble et al J Biol Chem. 1998 Nov 13;273(46):30524-9

Insert

PI-SceI

Use

Tags

Expression

Bacterial

Mutation

A1463G, A1464C

Promoter

T7

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag Hygro EGFP cLAP +2

Plasmid

#194310

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

cLap(EGFP)-P2A-Hygro

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag Hygro EGFP cLAP +0

Plasmid

#194308

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

cLap(EGFP)-P2A-Hygro

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag Hygro EGFP cLAP -2

Plasmid

#194313

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

cLap(EGFP)-P2A-Hygro

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag Hygro EGFP cLAP -0

Plasmid

#194311

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

cLap(EGFP)-P2A-Hygro

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag Hygro EGFP cLAP +1

Plasmid

#194309

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

cLap(EGFP)-P2A-Hygro

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag Hygro EGFP cLAP -1

Plasmid

#194312

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

cLap(EGFP)-P2A-Hygro

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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Stim1NTD-ABHD16Acyto-mNG

Plasmid

#195158

Purpose

mammalian expression of ER localized ABHD16A tagged with mNeonGreen

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

Stim1NTD (STIM1 Human)

Use

Tags

ABHD16A cytoplasmic domain and mNeonGreen

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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p8271 LentiCRISPR v2 hygro sgSIGIRR-4

Plasmid

#193980

Purpose

Expression of spCas9 and sgRNA targeting SIGIRR

Depositor

Elizabeth White

Article

Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

Insert

spCas9 and sgRNA targeting SIGIRR (SIGIRR Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pRD411

Plasmid

#178196

Purpose

sRok overexpression

Depositor

Remus Dame

Article

Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

Insert

srok

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pRD231

Plasmid

#178195

Purpose

Rok overexpression

Depositor

Remus Dame

Article

Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

Insert

rok

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCK411.RR1

Plasmid

#192644

Purpose

Expresses sgRNA RR1 (target mRFP CDS) on ColE1-AmpR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Insert

BBa_J23119-RR1

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

BBa_J23119

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pIALD2S

Plasmid

#185865

Purpose

Knocking out ALD6 and expressing anacetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP ) in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

Insert

ALD6 (-125, 40)> PADH1> Lr.pduP> TPDC1-ALD6 (1054, 1749)

Use

Tags

Expression

Yeast

Mutation

None

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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KBB439

Plasmid

#185093

Purpose

NMA111-GFP wild type under control of GAL1 promoter (complements nma111 deletion)

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Use

Tags

GFP

Expression

Yeast

Mutation

GAL1::NMA111-GFP

Promoter

GAL1

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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