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BASIC_SEVA_5a7.10

Plasmid

#178935

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Article

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pRS_1N_400

Plasmid

#170476

Purpose

To amplify transcription template containing LacI operators

Depositor

Laura Finzi

Article

Xu et al Nucleic Acids Res. 2022 Mar 21;50(5):282

Insert

T7A1, O1, O-, Lambda t1 in lacZ fragment

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7A1

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pTY1-LacI

Plasmid

#164831

Purpose

LacI protein regulates the expression of a tetracycline resistance gene and yellow fluorescent protein

Depositor

Drew Tack

Article

Tack et al Mol Syst Biol. 2021 Mar;17(3):e10179. do

Insert

LacI

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Ptac

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-Sox3gRNA-T1

Plasmid

#127777

Purpose

Chicken sox3 gRNA target site 1, cloned into the pU6 sgRNA (backbone #92359)

Depositor

Tatjana Sauka-Spengler

Article

Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

Insert

sox3gRNAT1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-Sox3gRNA-T2

Plasmid

#127778

Purpose

Chicken sox3 gRNA target site 2, cloned into the pU6 sgRNA (backbone #92359)

Depositor

Tatjana Sauka-Spengler

Article

Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

Insert

sox3gRNAT2

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pJUMP19-Ptet_P

Plasmid

#127005

Purpose

Basic Part P- Promoter (non-constitutive); Ptet; induced by tetracycline/analogue; controlled by TetR

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Ptet_P

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pstb-LAFR5-exoY

Plasmid

#86170

Purpose

pstb-LAFR5 with the S. meliloti 1021 exoY fragment from E6 cloned into Pst/Bam

Depositor

Kay Jones

Article

Jones et al J Bacteriol. 2012 Aug;194(16):4322-31. d

Insert

exoY

Use

Tags

Expression

Bacterial

Mutation

Promoter

Smb21651

Available since

Feb. 6, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pCVD518

Plasmid

#66158

Purpose

Six copies of the 415 bp PstI fragment of pCVD512, carrying the tdh1 gene probe from Vibrio parahaemolyticus WP1, for generation of probe

Depositor

James Kaper

Article

Nishibuchi et al Infect Immun. 1985 Sep;49(3):481-6.

Insert

tdh1

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Unknown

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pCVD402

Plasmid

#66148

Purpose

to make a probe to detect Heat stable enterotoxin gene (human)

Depositor

James Kaper

Article

Nishibuchi et al J Clin Microbiol. 1988 Apr;26(4):784-6.

Insert

STh

Use

Cloning vector

Tags

Expression

Mutation

Promoter

none

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pNH052

Plasmid

#42158

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

YFP-RT-YFP

Use

Tags

Expression

Mutation

Promoter

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pNH054

Plasmid

#42160

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1-RT-mTFP1

Use

Tags

Expression

Mutation

Promoter

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pMVV207

Plasmid

#215343

Purpose

gRNA expression for S. stutzeri nifL deletion on an RSF1010 backbone

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

S. stutzeri nifL gRNA

Use

CRISPR and Synthetic Biology

Tags

None

Expression

Bacterial

Mutation

Promoter

J23119

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJM253 (miniCTX1-rhaSR-PrhaBAD)

Plasmid

#110571

Purpose

Integration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoter

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

rhaSR-PrhaBAD

Use

Tags

Expression

Bacterial

Mutation

Promoter

rhaSR-PrhaBAD

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pSOUP

Plasmid

#165419

Purpose

The pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in Agrobacterium

Depositor

Wendy Harwood, John Innes Centre - Crop Transformation and Genome Editing Group

Article

Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.

Insert

Rep A

Use

Helper plasmid required for replication for all p…

Tags

Expression

Mutation

Promoter

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-GCaMP6s

Plasmid

#166606

Purpose

Encodes Cre-dependent GCaMP6s under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

GCaMP6s

Use

AAV

Tags

Expression

Mutation

Promoter

tetracycline response element

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ133B

Plasmid

#69283

Purpose

Golden Gate entry vector; 3rd gRNA under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ131B

Plasmid

#69281

Purpose

Golden Gate entry vector; 1st gRNA under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ132B

Plasmid

#69282

Purpose

Golden Gate entry vector; 2nd gRNA under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ134B

Plasmid

#179216

Purpose

Golden Gate entry vector; 4th gRNA under AtU3 promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

AtU3

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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Human ICAM-1

Plasmid

#8632

Depositor

Timothy Springer

Article

Staunton et al Cell 1988 Mar 25;52(6):925-33.

Insert

ICAM-1 (ICAM1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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