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pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7f-T2A-tdTomato-p10

Plasmid

#172919

Purpose

LexA activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Insert

jGCaMP7f-T2A-tdTomato

Use

Tags

6xHis and T2A-tdTomato

Expression

Insect

Mutation

Promoter

hsp70

Available since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pOTTC810 - pAAV-EF1a-CRTsigpep-GCaMP3 (D324G+D360G+397G+D435G 10.19)-KDEL

Plasmid

#63885

Purpose

An AAV packaging vector that expresses ER-retained low-affinity GCaMP3 variant under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

Insert

ER-localized low-affinity GCaMP3(10.19)

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pOTTC814 - pAAV-SYN1-CRTsigpep-GCaMP3 (D324G+D360G+397G+D435G 10.19)-KDEL

Plasmid

#63887

Purpose

An AAV packaging vector that expresses ER-retained low-affinity GCaMP3 variant under control of the SYN1 promoter.

Depositor

Brandon Harvey

Article

Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

Insert

ER-localized low-affinity GCaMP3(10.19)

Use

AAV

Tags

Expression

Mutation

Promoter

human SYN1

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-tdTomato

Plasmid

#44332

Depositor

Baljit Khakh

Article

Shigetomi et al J Gen Physiol. 2013 Apr 15.

Has Service

AAV5

Insert

tdTomato

Use

AAV; Adeno-associated virus ()

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV.GfaABC1D.PI.Lck-GFP.SV40

Plasmid

#105598

Purpose

AAV expression of membrane bound EGFP from GfaABC1D promoter

Depositor

Baljit Khakh

Article

Shigetomi et al J Gen Physiol. 2013 Apr 15.

Has Service

AAV5

Insert

Lck-EGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

GfaABC1D

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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G-CaMP3

Plasmid

#22692

Purpose

a single-wavelength GCaMP2-based genetically encoded calcium indicator

Depositor

Loren Looger

Article

Tian et al Nat Methods. 2009 Dec;6(12):875-81.

Insert

G-CaMP3

Use

Tags

Expression

Mammalian

Mutation

N363D mutation (increased fluorescence)

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pJH3971

Plasmid

#177736

Purpose

Prgef-1 GCaMP6::3xNLS::mNeptune

Depositor

Mei Zhen

Article

Susoy et al Cell. 2021 Sep 30;184(20):5122-5137.e17.

Insert

GCaMP

Use

Tags

mNeptune

Expression

Worm

Mutation

Promoter

Prgef-1

Available since

Dec. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pyc111s

Plasmid

#158781

Purpose

ribosome-tagged, Cre-dependent GCaMP for soma localization

Depositor

Zachary Knight, Jennifer Garrison

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

GCaMP6s-RPL10a

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pyc126m

Plasmid

#158777

Purpose

ribosome-tagged GCaMP for soma localization

Depositor

Zachary Knight, Jennifer Garrison

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

GCaMP6m-RPL10a

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pyc111f

Plasmid

#158780

Purpose

ribosome-tagged, Cre-dependent GCaMP for soma localization

Depositor

Zachary Knight, Jennifer Garrison

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

GCaMP6f-RPL10a

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pyc111m

Plasmid

#158779

Purpose

ribosome-tagged, Cre-dependent GCaMP for soma localization

Depositor

Zachary Knight, Jennifer Garrison

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

GCaMP6m-RPL10a

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pJH4260

Plasmid

#191678

Purpose

Ptwk-40s-GCaMP::wScarlet unc-54 3' UTR C.elegans AVA/other neurons expression GCaMP6s wScarlet

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

GCaMP6s

Use

Tags

wScarlet

Expression

Worm

Mutation

Promoter

Ptwk-40

Available since

Nov. 30, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pyc128m

Plasmid

#158778

Purpose

soluble GCaMP expression

Depositor

Zachary Knight, Jennifer Garrison

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

GCaMP6m

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAS18

Plasmid

#158787

Purpose

Ribosome-tagged GCaMP (soma localized) expression pan-neuronally C. elegans

Depositor

Zachary Knight, Jennifer Garrison

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

Ptag-168::GCaMP6m::rpl-1a

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAS19

Plasmid

#158788

Purpose

Ribosome-tagged GCaMP (soma localized) expression in C. elegans ASH neurons

Depositor

Zachary Knight, Jennifer Garrison

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

psra-6::GCaMP6m::rpl-1a

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAS16

Plasmid

#158786

Purpose

Ribosome-tagged GCaMP (soma localized) expression in C. elegans AFD neurons (and others)

Depositor

Zachary Knight, Jennifer Garrison

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

Pntc-1::GCaMP6m::rpl-1a

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHJ4

Plasmid

#158785

Purpose

Pan-neuronal nuclear-localized GCaMP expression in C. elegans

Depositor

Zachary Knight, Jennifer Garrison

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

Prab-3::nls::GCaMP6m

Use

Tags

NLS

Expression

Worm

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Salsa6f

Plasmid

#140188

Purpose

Salsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)

Depositor

Michael Cahalan

Article

Dong et al eLife. 2017 Dec 14;6. pii: 32417. doi: 1

Insert

Salsa6f

Use

Tags

tdTomato-v5-GCaMP6f

Expression

Mammalian

Mutation

Promoter

CMV Immediate Early

Available since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GenEPi

Plasmid

#140236

Purpose

Mammalian expression of Piezo1-based fluorescent force sensor GenEPi

Depositor

Periklis Pantazis

Article

Yaganoglu et al Nat Commun. 2023 Jul 19;14(1):4352. doi:

Insert

GenEPi (Piezo1-based fluorescent force sensor) (PIEZO1 Human)

Use

Tags

GCaMP 6s RS1 EF4

Expression

Mammalian

Mutation

Promoter

simian CMV IE94

Available since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-LUCI-GECO1

Plasmid

#113675

Purpose

Genetically-encoded ratiometric bioluminescent Ca2+ indicator based on GCaMP6s

Depositor

Robert Campbell

Article

Qian et al Chembiochem. 2018 Jun 22. doi: 10.1002/c

Insert

LUCI-GECO1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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