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Tet-O-FUW-STAT4-P2A-mCherry

Plasmid

#203853

Purpose

Tet-inducible lentiviral plasmid expressing STAT4-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

STAT4 (STAT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pTMB352_ZFcharm_Kv1_split-inteins

Plasmid

#220848

Purpose

Lentiviral vector expressing ZFcharm Kv2 fused to ZF3 and MmZFP_81187 via split Npu inteins; perfect match ZF3 binding site located upstream of pEFS

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm Kv2

Use

Lentiviral

Tags

P2A-mCherry

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4

Plasmid

#217893

Purpose

Insect cell expression of human 20S CP proteasome subunit alpha type-1 (PSMA1), alpha type-2 (PSMA2), alpha type-3 (PSMA3), and alpha type-4 (PSMA4)

Depositor

Brenda Schulman

Article

Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

Inserts

PSMA1 (PSMA1 Human)
PSMA2 (PSMA2 Human)
PSMA3 (PSMA3 Human)
PSMA4 (PSMA4 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pTER RB1shRNA human

Plasmid

#66886

Purpose

Doxycycline-regulated mammalian expression vector for expressing shRNA against RB1

Depositor

Maria Alvarado-Kristensson

Article

Ehlen et al J Biol Chem. 2012 May 18;287(21):17241-7

Insert

retinoblastoma 1 (RB1 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1 Tet

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
Tet-O-FUW-TRPS1-P2A-mCherry

Plasmid

#203851

Purpose

Tet-inducible lentiviral plasmid expressing TRPS1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

TRPS1 (TRPS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-Sec22b-P33-shR

Plasmid

#208359

Purpose

Mammalian expression of fluorescent N-terminally tagged shRNA resistant Sec22b-P33 mutant

Depositor

Paula Nunes-Hasler

Article

Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

Insert

Sec22b (Sec22b Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

four silent mutations (shRNA resistance), 33 aa p…

Promoter

CMV

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX2T-TUBG1

Plasmid

#171967

Purpose

pGEX2T-TUBG1 bacterial expression plasmid for production of recombinant GST fusion protein

Depositor

Maria Alvarado-Kristensson

Article

Corvaisier et al Commun Biol. 2021 Jun 22;4(1):767. doi:

Insert

TUBG1 (TUBG1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

R275H (Please see depositor comments)

Promoter

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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gamma-tubulin (1-335)

Plasmid

#226519

Purpose

Expressing a fragment of gamma-tubulin containing gamma-tubulin GTPase domain

Depositor

Maria Alvarado-Kristensson

Article

Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

Insert

TUBG1 N-terminal amino acids 1-335 (TUBG1 Human)

Use

Pcdna3.1

Tags

Expression

Mammalian

Mutation

Deleted amino acids 334-451 and introduced the fo…

Promoter

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-PBX4-P2A-mCherry

Plasmid

#203864

Purpose

Tet-inducible lentiviral plasmid expressing PBX4-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

PBX4 (PBX4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 E2F1 Flag(Δ193-359) (HindIII- BamHI- EcoRI)

Plasmid

#70667

Purpose

Human mutant of E2F1 lacking amino acids 193-359

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

E2F1 lacking residues 193 to 359 (E2F1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

lacks amino acids 193-359

Promoter

cmv

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 E2F1 Flag(Δ 284−359)(HindIII- BamHI- EcoRI)

Plasmid

#70669

Purpose

Human mutant of E2F1 lacking amino acids 284 to 359

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

E2F1 lacking residues 284 to 359 (E2F1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

lacks amin acids 284 to 359

Promoter

cmv

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only
Tet-O-FUW-ZEB2-P2A-mCherry

Plasmid

#203857

Purpose

Tet-inducible lentiviral plasmid expressing ZEB2-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

ZEB2 (ZEB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-NR4A3-P2A-mCherry

Plasmid

#203863

Purpose

Tet-inducible lentiviral plasmid expressing NR4A3-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

NR4A3 (NR4A3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-NR4A1-P2A-mCherry

Plasmid

#203859

Purpose

Tet-inducible lentiviral plasmid expressing NR4A1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

NR4A1 (NR4A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pACEBac1-POMP-PSMG1-PSMG2-PSMG3-PSMG4

Plasmid

#214137

Purpose

Insect cell expression of the human 20SCP proteasome assembly chaperones

Depositor

Brenda Schulman

Article

Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

Inserts

POMP (POMP Human)
PSMG1 (PSMG1 Human)
PSMG2 (PSMG2 Human)
PSMG3 (PSMG3 Human)
PSMG4 (PSMG4 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII

Plasmid

#217897

Purpose

Insect cell expression of human 20S CP proteasome subunit beta type-1 (PSMB1), beta type-2 (PSMB2), beta type-3 (PSMB3), and beta type-4 (PSMB4) with C-terminal Twin-Strep-tag

Depositor

Brenda Schulman

Article

Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

Inserts

PSMB1 (PSMB1 Human)
PSMB2 (PSMB2 Human)
PSMB3 (PSMB3 Human)
PSMB4 (PSMB4 Human)

Use

Tags

2xSTII

Expression

Insect

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A

Plasmid

#51248

Purpose

Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli3 (Gli3 Mouse)

Use

Flpin system

Tags

3xHA-TEV and Flag

Expression

Mammalian

Mutation

amino acids 849,865,877,907,980,1006 mutated to A…

Promoter

EF1a

Available since

March 7, 2014

Availability

Academic Institutions and Nonprofits only
Tet-O-FUW-NR3C1-P2A-mCherry

Plasmid

#203854

Purpose

Tet-inducible lentiviral plasmid expressing NR3C1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

NR3C1 (NR3C1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli3/Flag P5-6A

Plasmid

#51249

Purpose

Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 980,1006 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli3 (Gli3 Mouse)

Use

Flpin system

Tags

3xHA-TEV and Flag

Expression

Mammalian

Mutation

amino acids 980,1006 mutated to Ala

Promoter

EF1a

Available since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only
pEF5/FRT/DEST-3xHA/mGli3/Flag P1-4A

Plasmid

#51247

Purpose

Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli3 (Gli3 Mouse)

Use

Flpin system

Tags

3xHA-TEV and Flag

Expression

Mammalian

Mutation

amino acids 849,865,877,907 mutated to Ala

Promoter

EF1a

Available since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only