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Plasmid#40192DepositorInsertMex-5 (S404A) (RNAi resistant) (mex-5 Nematode)
UseTagsDendra2 PAFP (sequence optimized for worm express…ExpressionWormMutationS404A; exon 2 recoded to be RNAi-resistantPromoter4.4 kb mex-5 promoter fragmentAvailable sinceSept. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJCC_084 Cas12i1 D647A
Plasmid#171671PurposeFor bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tagDepositorInsertCas12i1 D647A
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged aspartate 647 to alaninePromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHis8-4b-McDAH
Plasmid#140129PurposeExpresses McDAH with N-terminal 8xHis-tag in BL21 E. coliDepositorInsertDechloroacutumine halogenase from Menispermum canadense
UseTags8x-His, TEV protease site (ENLYFQ)ExpressionBacterialMutationPromoterT7Available sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprtR161Q
Plasmid#92413PurposehQPRTase harboring R161Q mutationDepositorInserthQPRTase (QPRT Human)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationR161QPromoterT7Available sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEHIAhqprtR161G
Plasmid#92412PurposehQPRTase harbouring R161G mutationDepositorInserthQPRTase (QPRT Human)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationR161GPromoterT7Available sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprtR102Q
Plasmid#92407PurposehQPRTase harboring R102Q mutationDepositorInserthQPRTase (QPRT Human)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationR102QPromoterT7Available sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprtK171S
Plasmid#92404PurposehQPRTase harbouring K171S mutationDepositorInserthQPRTase (QPRT Human)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationK171SPromoterT7Available sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprtR138Q
Plasmid#92411PurposehQPRTase harbouring R138Q mutationDepositorInserthQPRTase (QPRT Human)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationR138QPromoterT7Available sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprtR102A
Plasmid#92405PurposehQPRTASE harbouring R102A mutationDepositorInserthQPRTase (QPRT Human)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationR102APromoterT7Available sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEG634
Plasmid#40121DepositorInsertMex-5 (aa244-468) (mex-5 Nematode)
UseGateway destination vectorTagsDendra2 PAFP (sequence optimized for worm express…ExpressionWormMutationcontains aa244-468Promoter4.4 kb mex-5 promoter fragmentAvailable sinceSept. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMSP1E3D1
Plasmid#20066PurposeBacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formationDepositorInsertMSP1E3D1
UseTags7-HisExpressionBacterialMutation"extended" MSP1D1; contains repeats of …PromoterAvailable sinceJan. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
Fireworks RFP[PTC+] (AVA2626)
Plasmid#85444PurposeExpresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.DepositorInserttDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-HygromycinR
UseMinimal backbone fragment for low-copy bacterial …TagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJCC_076 AsCas12a R1226A
Plasmid#171669PurposeFor bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tagDepositorInsertAsCas12a R1226A
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged arginine 1226 to alaninePromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-RBP-LOV2
Plasmid#185792PurposeEncodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linkerDepositorInsertRBP-LOV2
UseTags10xHis, TEV protease cleavage site after His TagExpressionBacterialMutationPromoterT7Available sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Fireworks GFP[PTC-] (AVA2598)
Plasmid#85445PurposeExpresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.DepositorInserttDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
UseMinimal backbone fragment for low-copy bacterial …TagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMBPCas2/3
Plasmid#89238PurposePlasmid contains the Cas2/3 gene from Pseudomonas aeruginosa PA14, with N-terminal Strep II tag, TEV protease cleavage site, and MBP fusionDepositorInsertCas2/3
UseTagsStrep II-MBPExpressionMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
Fireworks GFP[PTC+] (AVA2600)
Plasmid#85446PurposeExpresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.DepositorInserttDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
UseMinimal backbone fragment for low-copy bacterial …TagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJCC_075 AsCas12a D908A
Plasmid#171668PurposeFor bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tagDepositorInsertAsCas12a D908A
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged aspartate 908 to alaninePromoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP-Leu2d_GgPCFT
Plasmid#165414Purposeexpression GgPCFT protein with C terminally tagged GFP-His tag cleavable with TEV protease.DepositorInsertGgPCFT (SLC46A1 Synthetic, Chicken)
UseTagsGFP-His8ExpressionYeastMutationPromoterGAL1Available sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-aδNb072
Plasmid#204627PurposeExpresses the anti-IgD nanobody 072 (aδNb072) in the bacterial periplasm. aδNb072 is an orthosteric inhibitor of human anti-Phl p 7 antibodies of clone 102.1F10.DepositorInsertaδNb072
UseTagsHis6 and TEV protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only