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Showing: 441 - 454 of 454 results

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pRosa26-GT

Plasmid

#40025

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
beta-globin intron
Neo
tdT-3Myc
diphteria toxin A

Use

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta globin promoter and CMV enhance…

Availability

Academic Institutions and Nonprofits only

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pCMV-SpdCas9-VP64

Plasmid

#115794

Purpose

Expresses SpdCas9-Vp64 fusion protein when packaged into AAV

Depositor

Nadav Ahituv

Article

Matharu et al Science. 2018 Dec 13. pii: science.aau06

Insert

VP64

Use

AAV

Tags

3XFLAG and dCas9-VP64

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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HS-DN-Ect2

Plasmid

#105956

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Dzafic et al Cell Rep. 2015 Oct 6;13(1):168-182. doi:

Insert

Ect2 (ECT2 Human)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-pMagHigh-FlpC

Plasmid

#180586

Purpose

PA-Flp

Depositor

Won Do Heo

Article

Jung et al Nat Commun. 2019 Jan 18;10(1):314. doi:

Insert

Split FlpO

Use

AAV

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-mCherry-P2A-NLSFlpN-nMagHigh

Plasmid

#180585

Purpose

PA-Flp

Depositor

Won Do Heo

Article

Jung et al Nat Commun. 2019 Jan 18;10(1):314. doi:

Insert

Split FlpO

Use

AAV

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pBT272_(pRosa26-GTET)

Plasmid

#36882

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tTA2
beta-globin intron
Neo
tdT-3Myc
insulator
diphteria toxin A

Use

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Availability

Academic Institutions and Nonprofits only

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RFP-PASS

Plasmid

#193971

Purpose

Monitoring the production of phosphatidic acid with RFP-PASS (phosphatidic acid biosensor with superior sensitivity)

Depositor

Guangwei Du

Article

Zhang et al Mol Cell Biol. 2014 Jan;34(1):84-95. doi

Insert

PA biosenor

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX-GTB

Plasmid

#26197

Depositor

Edward Callaway

Article

Haubensak et al Nature. 2010 Nov 11. 468(7321):270-6.

Insert

Enhanced green fluorescent protein, TVA, rabies B19 glycoprotein

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

Expression

Mutation

TVA, rabies glycoprotein are mammalian codon opti…

Promoter

Availability

Academic Institutions and Nonprofits only

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EF1α-Lox2272-LoxP-MARKS·mTurquoise2-NLS·turboRFP-Lox2272-LoxP

Plasmid

#198753

Purpose

Cre reporter, which switches from membraneous mTurquoise to nuclear RFP upon Cre recombinase activity

Depositor

Jacco van Rheenen

Article

Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:

Insert

EF1a promotor-Lox2272-LoxP-MARKS-mTurquoise2-NLS.turboRFP-Lox2272-LOXP

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

See depositor comments below

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only

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pbG-APRT-J-u32uni-PdTKmCh

Plasmid

#107279

Purpose

APRT c.407T>C (APRT*J) donor vector with unilateral microhomology

Depositor

Knut Woltjen

Article

Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

Inserts

APRT (APRT Human)
puro-deltaTK; CAG::mCherry

Use

Tags

Expression

Mutation

c.402A>T, c.407T>C

Promoter

Availability

Academic Institutions and Nonprofits only

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pSYC-201

Plasmid

#178963

Purpose

Human GFAP fused to 3xFLAG tag and EGFP driven by zebrafish gfap promoter for Tol2 mediated recombination

Depositor

Seok-Yong Choi

Article

Lee et al BMC Neurol. 2017 Sep 7;17(1):175. doi: 1

Inserts

Glial fibrillary acidic protein promoter (gfap Zebrafish)
Glial fibrillary acidic protein (GFAP Human)
EGFP-pA

Use

Tol2 mediated recombination

Tags

3xFLAG

Expression

Mutation

Promoter

zebrafish gfap promoter

Availability

Academic Institutions and Nonprofits only

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pSYC-204

Plasmid

#178964

Purpose

Human GFAP (R79C) mutant fused to 3xFLAG tag and EGFP driven by zebrafish gfap promoter for Tol2 mediated recombination

Depositor

Seok-Yong Choi

Article

Lee et al BMC Neurol. 2017 Sep 7;17(1):175. doi: 1

Inserts

Glial fibrillary acidic protein promoter (gfap Zebrafish)
Glial fibrillary acidic protein (GFAP Human)
EGFP-pA

Use

Tol2 mediated recombination

Tags

3xFLAG

Expression

Mutation

R79C

Promoter

Zebrafish gfap promoter

Availability

Academic Institutions and Nonprofits only

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pAAV-Ai14-luc

Plasmid

#87118

Purpose

AAV backbone plasmid including luc-pA knock-in donor and Ai14gRNA for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

U6-Ai14sgRNA-Luciferase-nEF-GFPKASH-hGHpA

Use

AAV, CRISPR, and Luciferase

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAAV-rMERTK-HITI

Plasmid

#87119

Purpose

Gene correction donor AAV for RCS rat. AAV backbone plasmid including exon 2 of rat Mertk and rMertkgRNA for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

U6-rMertksgRNA-Mertk(intron1-2)-nEF-GFPKASH-pA

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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