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Plasmid#87117PurposeAAV backbone plasmid including GFPNLS-pA knock-in donor and Ai14gRNA for HITIDepositorInsertU6-Ai14sgRNA-GFPNLS-EF1a-mCherryKASH-pA
UseAAV and CRISPRTagsExpressionMutationPromoterAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPAS-E
Plasmid#39867DepositorInsertiRFP protein PAS domain
UseTagsE coilExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMma PylT sfGPF 150TAG
Plasmid#154766Purposeplasmid with 4xMma PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
UseTagsExpressionMammalianMutation150TAG in GFP reporter, U25C in PylTPromoterEF1Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_sfGPF 150TAG
Plasmid#154765Purposeplasmid with amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
UseTagsExpressionMammalianMutation150TAGPromoterEF1Available sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRosa26-TG
Plasmid#40026DepositorInsertsT (ATG)
beta-globin intron
Neo
GFP
diphteria toxin A
UseTagsExpressionMammalianMutationdeleted nucleotides 1-274 and insertion of FRT, …PromoterCAG (chicken beta globin promoter and CMV enhance…Available sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FLEX-GT
Plasmid#26198DepositorInsertEnhanced green fluorescent protein, TVA (eGFP TVA is a mouse codon optimized version of an avian gene., Mouse)
UseAAV and Cre/LoxTagsExpressionMutationTVA mammalian codon optimizedPromoterAvailable sinceSept. 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRosa26-GT
Plasmid#40025DepositorInsertsGFP
beta-globin intron
Neo
tdT-3Myc
diphteria toxin A
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta globin promoter and CMV enhance…Available sinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBT259_(pRosa26-G-tTA2)
Plasmid#36878DepositorInsertsGFP
tTA2
beta-globin intron
Neo
diphteria toxin A
UseTagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SpdCas9-VP64
Plasmid#115794PurposeExpresses SpdCas9-Vp64 fusion protein when packaged into AAVDepositorInsertVP64
UseAAVTags3XFLAG and dCas9-VP64ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
HS-DN-Ect2
Plasmid#105956Purposeheat shock inducible transgenesisDepositorInsertEct2 (ECT2 Human)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-pMagHigh-FlpC
Plasmid#180586PurposePA-FlpDepositorInsertSplit FlpO
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-mCherry-P2A-NLSFlpN-nMagHigh
Plasmid#180585PurposePA-FlpDepositorInsertSplit FlpO
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBT272_(pRosa26-GTET)
Plasmid#36882DepositorInsertsGFP
tTA2
beta-globin intron
Neo
tdT-3Myc
insulator
diphteria toxin A
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
RFP-PASS
Plasmid#193971PurposeMonitoring the production of phosphatidic acid with RFP-PASS (phosphatidic acid biosensor with superior sensitivity)DepositorInsertPA biosenor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FLEX-GTB
Plasmid#26197DepositorInsertEnhanced green fluorescent protein, TVA, rabies B19 glycoprotein
UseAAV and Cre/Lox; Adeno-associated virusTagsExpressionMutationTVA, rabies glycoprotein are mammalian codon opti…PromoterAvailable sinceNov. 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
EF1α-Lox2272-LoxP-MARKS·mTurquoise2-NLS·turboRFP-Lox2272-LoxP
Plasmid#198753PurposeCre reporter, which switches from membraneous mTurquoise to nuclear RFP upon Cre recombinase activityDepositorInsertEF1a promotor-Lox2272-LoxP-MARKS-mTurquoise2-NLS.turboRFP-Lox2272-LOXP
UseLentiviralTagsExpressionBacterial and MammalianMutationSee depositor comments belowPromoterEF1aAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pbG-APRT-J-u32uni-PdTKmCh
Plasmid#107279PurposeAPRT c.407T>C (APRT*J) donor vector with unilateral microhomologyDepositorInsertsUseTagsExpressionMutationc.402A>T, c.407T>CPromoterAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSYC-201
Plasmid#178963PurposeHuman GFAP fused to 3xFLAG tag and EGFP driven by zebrafish gfap promoter for Tol2 mediated recombinationDepositorInsertsUseTol2 mediated recombinationTags3xFLAGExpressionMutationPromoterzebrafish gfap promoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSYC-204
Plasmid#178964PurposeHuman GFAP (R79C) mutant fused to 3xFLAG tag and EGFP driven by zebrafish gfap promoter for Tol2 mediated recombinationDepositorInsertsUseTol2 mediated recombinationTags3xFLAGExpressionMutationR79CPromoterZebrafish gfap promoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ai14-luc
Plasmid#87118PurposeAAV backbone plasmid including luc-pA knock-in donor and Ai14gRNA for HITIDepositorInsertU6-Ai14sgRNA-Luciferase-nEF-GFPKASH-hGHpA
UseAAV, CRISPR, and LuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only