Addgene

Showing: 441 - 460 of 1186 results

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sgRNA3 _1xCTS-ECFP-pA

Plasmid

#200239

Purpose

Mammalian transfections; 1xCTS-ECFP reporter construct 3

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA3_1xCTS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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sgRNA4 _1xCTS-ECFP-pA

Plasmid

#200240

Purpose

Mammalian transfections; 1xCTS-ECFP reporter construct 4

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA4_1xCTS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
sgRNA5 _1xCTS-ECFP-pA

Plasmid

#200241

Purpose

Mammalian transfections; 1xCTS-ECFP reporter construct 5

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA5_1xCTS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
sgRNA1 _8xCTS-ECFP-pA

Plasmid

#200243

Purpose

Mammalian transfections; 8xCTS-ECFP reporter construct 1

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA1_8xCTS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
gRNA4 _8xCTS-ECFP-pA

Plasmid

#200246

Purpose

Mammalian transfections; 8xCTS-ECFP reporter construct 4

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA4_8xCTS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
lvl0 eCFP CDS

Plasmid

#153397

Purpose

Cyan fluorescent protein coding sequence

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

eCFP CDS

Use

Tags

Expression

Plant

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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ECFP-Rab5C-S35N

Plasmid

#110504

Purpose

Dominant Negative

Depositor

Bernardo Mainou, Terence Dermody

Article

Mainou et al J Virol. 2012 Aug;86(16):8346-58. doi: 1

Insert

Rab5C-S35N (RAB5C Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

S35N

Promoter

Availability

Academic Institutions and Nonprofits only

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PIP2-CFP(ST) sensor

Plasmid

#170355

Purpose

CFP(ST)-PH fusion used to measure realtime changes in PIP2 levels.

Depositor

Kees Jalink

Article

van der Wal et al J Biol Chem. 2001 May 4. 276(18):15337-4

Insert

CFP(ST)-PH (PLCD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pQC-FsCFP-mCherryFP

Plasmid

#154836

Purpose

Frame-shift fluorescent reporter for genomic DNA editing

Depositor

Fangliang Zhang

Article

Kumar et al BMC Genomics. 2019 Jul 24;20(1):609. doi

Insert

targeting sequence of VEGF

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti sgRNA CFP

Plasmid

#155281

Purpose

Lentiviral sgRNA cloning with CFP marker

Depositor

Martin Carroll

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAFNF-ECFP-Actb

Plasmid

#169787

Purpose

An actin FRET sensor. Must be expressed with Venus-actin for FRET.

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Actb (Actb Mouse)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pH2B-SCFP1-2A-mTq2-NES

Plasmid

#60491

Purpose

Co-expresses two cyan fluorescent protein variants, a short fluorescence lifetime CFP variant in the nucleus and a CFP variant with a long lifetime (mTurquoise2) in the cytoplasm

Depositor

Dorus Gadella

Article

Joosen et al J Microsc. 2014 Sep 1. doi: 10.1111/jmi.

Inserts

H2B-SCFP1
mTurquoise2-NES

Use

Tags

SCFP1 and mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
mGDF8 donor CFP-hDF

Plasmid

#72590

Purpose

Plasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

Depositor

Renzhi Han

Article

Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

Insert

dysferlin (DYSF Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
hGDF8 donor CFP-hDF

Plasmid

#72591

Purpose

Plasmid contains left and right homology arms for human GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

Depositor

Renzhi Han

Article

Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

Insert

dysferlin (DYSF Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
Lck-FRB-ECFP(W66A)

Plasmid

#67902

Purpose

Expresses a membrane located FRB domain in mammalian cells (the ECFP is not fluorescent)

Depositor

Dorus Gadella

Article

van_Unen et al Scientific Reports 5, Article number: 14

Insert

Lck-FRB-ECFP(W66A)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
FRB-ECFP(W66A)-Giantin

Plasmid

#67903

Purpose

Expresses a Golgi located FRB domain in mammalian cells (the ECFP is not fluorescent)

Depositor

Dorus Gadella

Article

van_Unen et al Scientific Reports 5, Article number: 14

Insert

FRB-ECFP(W66A)-Giantin

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
ECFP(W66A)-FRB-MoA

Plasmid

#67904

Purpose

Expresses a Mitochondria located FRB domain in mammalian cells (the ECFP is not fluorescent)

Depositor

Dorus Gadella

Article

van_Unen et al Scientific Reports 5, Article number: 14

Insert

ECFP(W66A)-FRB-MoA

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
P4U2C8T2-v1-1 (Pl-tetO1-deCFP) (135p)

Plasmid

#70211

Purpose

1 Part of 4-part switch

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deCFP

Use

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
phage-cmv-cfp-24xms2

Plasmid

#40651

Depositor

Robert Singer

Article

Wu et al Biophys J. 2012 Jun 20;102(12):2936-44.

Insert

CFP-24xMBS

Use

Lentiviral

Tags

CFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
phage-cmv-cfp-24xpp7

Plasmid

#40652

Depositor

Robert Singer

Article

Wu et al Biophys J. 2012 Jun 20;102(12):2936-44.

Insert

CFP-24xPP7

Use

Lentiviral

Tags

CFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge