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Plasmid#63828PurposeThis plasmid is designed to validate the biophysical model of translational couplingDepositorInsertCFP, mRFP1 and GFPmut3b
UseTagsExpressionBacterialMutationThe intergenic distance between each gene is -4 n…PromoterJ23100Available sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHL1017
Plasmid#62828PurposeLim Lab plasmid: AmpR + PConNoHindM8::glgC::gfp::T1T2 terminator + ColE1DepositorInsertgfp
UseSynthetic BiologyTagsm8::glgC leaderExpressionBacterialMutationPromoterpConNoHindAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
DSCR8_pCSdest
Plasmid#53828DepositorInsertDSCR8 (DSCR8 Human)
UseTagsExpressionMammalianMutationPromoterSP6 and CMVAvailable sinceSept. 9, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUAST YFP Rab8 WT
Plasmid#58450Purposeexpression of drosophila YFP Rab8DepositorInsertRab8 (Rab8 Fly)
UseTagsYFPExpressionInsectMutationPromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST td tomato Rab8 WT
Plasmid#58449Purposeexpression of drosophila td tomato Rab8DepositorInsertRab8 (Rab8 Fly)
UseTagstd TomatoExpressionInsectMutationPromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGWB442
Plasmid#74828PurposeGateway cloning compatible binary vector for N-terminal fusion with eYFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
OptoProfilin
Plasmid#208286PurposeExpresses Cry2(1-531)-mCherry-Profilin.WTDepositorUseTagsmCherryExpressionMammalianMutationProfilin WT (Mus musculus)PromoterCMVAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330 EGFR-sgRNA
Plasmid#188633PurposeA human codon-optimized SpCas9 and chimeric guide RNA targeting C-terminus of human EGFRDepositorInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pirespuro2-CTLA4-mEos2
Plasmid#98285Purposemammalian expression of CTLA4-mEos2DepositorInsertCTLA4 (CTLA4 Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pirespuro2-CD86-mEos2
Plasmid#98284Purposemammalian expression of CD86-mEos2DepositorInsertCD86 (CD86 Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-APEX.NES-P2A-EGFP
Plasmid#182824PurposeCre-dependent expression of cytosolic APEXDepositorInsertAPEX.NES-P2A-EGFP
UseAAVTagsEGFP reporter (P2A)ExpressionMutationPromoterEF1aAvailable sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/NS4A-2K-NS4B
Plasmid#120828PurposeEctopic expression of ZIKV proteinDepositorInsertNS4A-2K-NS4B ZIKV
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mNeonGreen-LEHD-NanoLuc
Plasmid#98289Purposecaspase-9 BRET SensorDepositorInsertmNeonGreen-LEHD-NanoLuc
UseTagsHis-tagExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
FKBP-CD28TMD-NTEVp(H75S)
Plasmid#137828PurposeMESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV proteaseDepositorInsertFKBP-CD28TMD-NTEVp(H75S)
UseTags3x FLAGExpressionMammalianMutationMutated Histidine 75 to Serine in NTEVp fragmentPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-hSt3Cas9 (pXK-1721)
Plasmid#208828PurposeCRISPR/hSt3Cas9 expression vector for Animal gene editing, harboring the humanized St3Cas9 gene and the corresponding optimized SP1.gRNA. Key Construct: mU6- SP1.gRNA-CMV-hSt3Cas9.DepositorTypeEmpty backboneUseCRISPR; Stcas9TagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFX-β-tubulin-Venus
Plasmid#175828PurposeExpress Venus with microtubule-targeting sequence in mammalian cellsDepositorInsertVenus with microtuble-targeting sequence
UseTagshuman tubulin beta 4B class IVb;TUBB4BExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTpal-fdc
Plasmid#78288PurposeExpresses A. thaliana PAL2 and S. cerevisiae FDC1 in E. coliDepositorUseTagsExpressionBacterialMutation*see belowPromotertrcAvailable sinceJune 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
miR-34aM2
Plasmid#50828Purposeluciferase reporter for human miR-34aDepositorInsertmiR-34A promoter M2 (MIR34A Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationsecond NF-kB binding site mutated (-511)PromotermiR34aAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-sgAAVS1-HepmTurquoise2
Plasmid#192828PurposeExpresses sgRNA targeting AAVS1 (non-targeting control sgRNA for mouse) and mTurquoise2 from hepatocyte-specific promoterDepositorInsertmTurquoise2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 for sgRNA and hepatocyte-specific promoter (HS…Available sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluSnFR
Plasmid#119828PurposeProduction of recombinant iGluSnFR, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluSnFR
UseTagsGSTExpressionBacterialMutationPromoterT7Available sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only