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Plasmid#209345PurposeProtein expression vector with a TEV cleavable N-terminal 6xHis-MBP and C-terminal StrepTagIIDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only
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pMal-HA
Plasmid#209346PurposeProtein expression vector with a TEV cleavable N-terminal 6xHis-MBP and C-terminal StrepTagIIDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGTM_YR375_SUMO_Protease_001
Plasmid#190063PurposeExpresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusionDepositorInsertULP1_YEAST (ULP1 Budding Yeast)
UseTags8X His-MBP (Maltose Binding Protein)ExpressionBacterialMutationConstruct contains residues 347 – 621PromoterT7 lacAvailable sinceJan. 30, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
HMA-APOBEC1-Nb
Plasmid#226012PurposeFor expression of His-MBP-APOBEC1-Nanobody protein in E.coliDepositorInsertAPOBEC1 (Apobec1 Rat)
UseTagsHis-MBP and antiRabbit-nanobodyExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
spCas9-NLS-MAV
Plasmid#124212PurposeSpCas9-NLS 17 amino acid linker to monoavidin (SpCas9-NLS-MAV)DepositorInsertcas9
UseCRISPRTagsHis6, MBP, TEV Cleavage site, and two copies of S…ExpressionBacterialMutationPromoterT7Available sinceJune 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM41_KEAP1_KELCH
Plasmid#178476PurposeBacterial expression of human domain with His-tag and MBP tagDepositorInsertKEAP1_KELCH (KEAP1 Synthetic, Human)
UseTagsHis-MBPExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHMTc-FBF-2 (121-632)
Plasmid#218664PurposeExpression of MBP:6xHIS:FBF-2(121-632)DepositorInsertfbf-2 (fbf-2 Nematode)
UseTagsMBP-6xHISExpressionBacterialMutationamino acids 121-632 onlyPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJK2097
Plasmid#218665PurposeExpression of MBP:6xHIS:FBF-2(Y479A, 121-632)DepositorInsertfbf-2 (fbf-2 Nematode)
UseTagsMBP-6xHISExpressionBacterialMutationamino acids 121-632 only, with Y479A mutationPromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM41_KPNA4_Arm
Plasmid#178478PurposeBacterial expression of human domain with His-tag and MBP tagDepositorInsertKPNA4_Arm (KPNA4 Synthetic, Human)
UseTagsHis-MBPExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c2X-ORF24-NTD-3L_A
Plasmid#138466PurposeExpresses MBP-tagged ORF24-NTD 3L_A in bacteriaDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsMBPExpressionBacterialMutationAmino acids 1-201; amino acids L73-75 mutated to …PromotertacAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMal-Halo
Plasmid#209349PurposeProtein expression vector with a TEV cleavable N-terminal 6xHis-MBP and a HRV 3C cleavable C-terminal HaloTag-StepTagIIDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMal-mRuby3
Plasmid#209348PurposeProtein expression vector with a TEV cleavable N-terminal 6xHis-MBP and a HRV 3C cleavable C-terminal mRuby3-StepTagIIDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest-566
Plasmid#11517DepositorTypeEmpty backboneUseGateway destination vectorTags6xHis and MBPExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pJJGL001
Plasmid#180605PurposePlasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasXDepositorInsertDpbCasX
UseCRISPRTags10x His and MBPExpressionBacterialMutationPromoterT7Available sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJJGL002
Plasmid#180606PurposePlasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasXDepositorInsertPlmCasX
UseCRISPRTags10xHis and MBPExpressionBacterialMutationPromoterT7Available sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-cFos-XRI-V5
Plasmid#178058PurposeExpression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with V5 tag, under c-fos promoter.DepositorInsertXRI-V5
UseAAVTagsV5-MBPExpressionMammalianMutationPromoterMouse c-fos promoterAvailable sinceOct. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBig1a zz TEV YBBR RAP1 ZZ TEV TRF2
Plasmid#185449PurposeCoexpresses human RAP1 with an MBP affinity tag, YBBR site, and TEV site, and human TRF2 with a zz tag and TEV siteDepositorUseTagsMBP, YBBR, and ZZExpressionInsectMutationPromoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-SpRY-NLS-6xHis (gbr2101)
Plasmid#181743Purposebacterial expression plasmid for SpRY with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tagDepositorInsertbacterial codon optimized SpRY
UseIn vitro transcription; t7 promoterTagsBPNLS(SV40)-6xHis and MKIEE MBP tagExpressionBacterialMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterT7Available sinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-XRI-HA
Plasmid#178056PurposeExpression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with HA tag, under UBC promoter.DepositorInsertXRI-HA
UseAAVTagsHA-MBPExpressionMammalianMutationPromoterHuman ubiquitin C (UBC) promoterAvailable sinceOct. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNS20-SpCas9-SNAP
Plasmid#113717PurposeBacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9DepositorInsertCas9
UseCRISPRTagsHA-2xNLS-SNAP-NLS and His6-MBP-TEVExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNS38-SadCas9-SNAP
Plasmid#113718PurposeBacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9DepositorInsertCas9
UseCRISPRTagsHA-2xNLS-SNAP-NLS and His6-MBP-TEVExpressionBacterialMutationD10A, N580APromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
MEF2A-Sensor
Plasmid#208928Purposein vitro MAPK-Sensor for luminescent detection of ERK and p38-activityDepositorInsertMef2a docking sequence+Phospho site (MEF2A Human)
UseTagsHISx6, MBP, and SmbitExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM41_KPNB1_HEAT
Plasmid#178494PurposeBacterial expression of human domain with His-tag and MBP tagDepositorInsertKPNB1_HEAT (KPNB1 Synthetic, Human)
UseTagsHis-MBPExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMal-SLFN2
Plasmid#174322PurposeBacterial expression of MBP-tagged mouse SLFN2DepositorInsertSLFN2 (Slfn2 Mouse)
UseTagsMBPExpressionBacterialMutationPromotertac promoterAvailable sinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCOG75-Sem1 Sc
Plasmid#83233PurposepCDF-His6-MBP-Sem1 ScDepositorInsertSEM 1 (SEM1 Budding Yeast)
UseTagsHis-mbpExpressionBacterialMutationPromoterAvailable sinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
FENEF-Sensor
Plasmid#208930Purposein vitro p38-Sensor for luminescent detection of p38-activityDepositorInsertFENEF sequence from human ATF2 with phospho site (ATF2 Human)
UseTagsHISx6, MBP, and SmbitExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL-p2x-LanCL2
Plasmid#154192PurposeTo express MBP-LanCL2 in bacterial cellsDepositorInsertLanCL2
UseTagsMaltose binding protein (MBP)ExpressionBacterialMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJJGL003
Plasmid#180607PurposePlasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX+Region3 insertionDepositorInsertDpbCasX with R3 loop
UseCRISPRTags10x His and MBPExpressionBacterialMutationPromoterT7Available sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM41-EcPPK
Plasmid#38334DepositorInsertE. coli polyphosphate kinase (ppk Escherichia coli)
UseTags6xHIS, MBP, and TEVExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28-SpRY-HF1-NLS-6xHis (gbr2102)
Plasmid#181744Purposebacterial expression plasmid for SpRY-HF1 with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tagDepositorInsertbacterial codon optimized SpRY-HF1
UseIn vitro transcription; t7 promoterTagsBPNLS(SV40)-6xHis and MKIEE MBP tagExpressionBacterialMutationSpRY-HF1, SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E…PromoterT7Available sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-FLEX-XRI-FLAG
Plasmid#178057PurposeExpression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with FLAG tag, under UBC promoter with a FLEX switch.DepositorInsertXRI-FLAG
UseAAVTagsFLAG-MBPExpressionMammalianMutationPromoterHuman ubiquitin C (UBC) promoterAvailable sinceOct. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMAL-SH3(2)-5R
Plasmid#112089PurposeBacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.DepositorInsertSH3(2)-5R (NCK1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationconstruct contains only repeats of the second SH3…PromoterLacAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJJGL004
Plasmid#180608PurposePlasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasX+Region1 insertionDepositorInsertPlmCasX with DpbCasX R1 loop
UseCRISPRTags10xHis and MBPExpressionBacterialMutationPromoterT7Available sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
K365-micro
Plasmid#188457PurposeComponent of optically-induced motor dimerization systemDepositorInsertKinesin 1-365 (Khc Fly)
UseTags6xHis-MBP-TEV and SspB microExpressionBacterialMutationKinesin amino acids 1-365PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMal_Abl-PRM 3R
Plasmid#112086PurposeBacterial expression plasmid containing His and MBP tags for 3 PRM motif repeats of Human Abl.DepositorInsertAbl-PRM-3R (ABL1 Human)
UseTagsHis-6 and MBPExpressionBacterialMutationconstruct contains only the PRM motif of Abl1PromoterLacAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMAL-Abl-PRM 4R
Plasmid#112087PurposeBacterial expression plasmid containing His and MBP tags for 4 PRM motif repeats of Human Abl.DepositorInsertAbl-PRM-4R (ABL1 Human)
UseTagsHis-6 and MBPExpressionBacterialMutationconstruct contains only the PRM motif of Abl1PromoterLacAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMal-Abl-PRM 5R
Plasmid#112088PurposeBacterial expression plasmid containing His and MBP tags for 5 PRM motif repeats of Human Abl.DepositorInsertPRM-5R (ABL1 Human)
UseTagsHis-6 and MBPExpressionBacterialMutationconstruct contains only the PRM motif of Abl1PromoterLacAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMB.BIG1a.PRC2
Plasmid#234581PurposeExpression of EZH2, MBP-SUZ12, EED, RBBP4DepositorUseTagsExpressionInsectMutationPromoterAvailable sinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pVP16-tev-PRT1
Plasmid#83405Purposebacterial expression vector pVP16 containing Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studiesDepositorInsertPROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
UseTagsHis8, MBP, and tev cleavage siteExpressionBacterialMutationPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only