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Plasmid#80059PurposeMCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;mafbbCE1-basEgfp
Plasmid#90137Purposecontains mafbb enhancer sequence and a basal promoter driving expression of eGFPDepositorInsertmafbbE1basEgfp
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T ZfHoxb10a
Plasmid#27530DepositorInsertHoxb10a (hoxb10a Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T ZfHoxd12a
Plasmid#27545DepositorInsertHoxd12a (hoxd12a Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
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actc1b-mKate2-EEA1
Plasmid#109508PurposeMuscle specific zebrafish expression of mKate2 fused to early endosome associated 1DepositorInsertEEA1 (eea1 Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-EHD2a
Plasmid#109516PurposeMuscle specific zebrafish expression of mKate2 fused to EHD2aDepositorInsertEHD2a (ehd2a Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Cre-cmlc2-TagBFP
Plasmid#117792PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertCre
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
M17 ptwhh-IGFP65C
Plasmid#17154DepositorInserttwhh promoter (shhb Zebrafish)
UseTagsb-globin intron, GFPExpressionMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
M16 pCS-twhh-bGFP
Plasmid#17153DepositorInserttwhh promoter (shhb Zebrafish)
UseTagsbGFPExpressionMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;basPEgfp
Plasmid#90169Purposecontains a basal promoter driving expression of eGFPDepositorInsertbasalPEgfp
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
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Zebrafish-gRNA-0005
Plasmid#42245DepositorInsertgRNA-gsk3b (gsk3b Zebrafish)
UseCRISPR; Zebrafish expressionTagsExpressionMutationPromoterT7Available sinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pGEM-myr-eGFP-SV40pA-FKF
Plasmid#119866PurposeDonor cassette containing myr-tagged eGFP and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertmyr-tagged eGFP and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
UseTags2x myristoylationExpressionBacterialMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF
Plasmid#119864PurposeDonor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertBirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
UseTagsHA-tagged biotin ligase (BirA), Thosea asigna vir…ExpressionBacterialMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
453 pCSMTN5ICD
Plasmid#22466DepositorInsertNotch3 (notch3 Zebrafish)
UseExpression vectorTagsExpressionMutationPromoterAvailable sinceSept. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
SI4-Jamc.2
Plasmid#28216DepositorInsertJamc.2 mouse monoclonal antibody (jam3a Zebrafish)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBS ZfHoxb1b
Plasmid#27520DepositorInsertHoxb1b (hoxb1b Zebrafish)
UseRiboprobe synthesisTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
Zebrafish-gRNA-0008
Plasmid#42248DepositorInsertgRNA-tia1l (tia1l Zebrafish)
UseCRISPR; Zebrafish expressionTagsExpressionMutationPromoterT7Available sinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only