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Plasmid#49001Purposefull length coding sequence for zebrafish fli1a gene in gateway middle entry vectorDepositorInsertfriend leukemia integration 1a (fli1 Zebrafish)
UseGateway middle entryTagsExpressionMutationPromoterAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
Rab18b cds in pGem-T Easy
Plasmid#80539PurposeCan be used for in situ hybridisation or other purposesDepositorInsertrab18b (rab18b Zebrafish)
UseTagsExpressionMutationPromoterAvailable sinceApril 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
Rab18a cds in pGem-T Easy
Plasmid#80538PurposeCan be used for in situ hybridisation or other purposesDepositorInsertrab18a (rab18a Zebrafish)
UseTagsExpressionMutationPromoterAvailable sinceApril 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pMT-ubb-NLS-BirA-2a-mCherry
Plasmid#79886PurposeUbiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseTags3x HAExpressionBacterialMutationPromoterUbiquitinAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
shha-pCRII
Plasmid#68962Purposefor antisense probe, NotI w/ Sp6DepositorInsertshha (shha Zebrafish)
UseTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-rab5c
Plasmid#109651PurposeMuscle specific zebrafish expression of mKate2 fused to rab5cDepositorInsertrab5c (rab5c Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-rab11ba
Plasmid#109596PurposeMuscle specific zebrafish expression of mKate2 fused to rab11baDepositorInsertrab11ba (rab11ba Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT-MCS-NLS-BirA-2A-mCherry_Ras
Plasmid#80059PurposeMCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA2-LA-nanoLuc-RA
Plasmid#128692PurposeDonor plasmid that promotes integration of the NanoLuc coding sequence at the apobb.1 locusDepositorInsertapobb.1 NanoLuc
UseMultisite-gateway assemblyTagsNanoLucExpressionMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj11
Plasmid#173139PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj11 (kcnj11 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;mafbbCE1-basEgfp
Plasmid#90137Purposecontains mafbb enhancer sequence and a basal promoter driving expression of eGFPDepositorInsertmafbbE1basEgfp
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-EHD1b
Plasmid#109514PurposeMuscle specific zebrafish expression of mKate2 fused to EHD1bDepositorInsertEHD1b (ehd1b Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
ptdTomato-N1-CMVL-ikk1FL(C179A)-tdTomato
Plasmid#105644PurposeZebrafish Inhibitor of kappa B kinase alpha with mutation at position 179 from cysteine to alanineDepositorInsertCMVL-ikk1FL(C179A)-tdTomato (chuk Zebrafish)
UseTagstdTomatoExpressionMammalianMutationCMV:ikk1FL(C178A)-tdTomatoPromoterAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-EHD2a
Plasmid#109516PurposeMuscle specific zebrafish expression of mKate2 fused to EHD2aDepositorInsertEHD2a (ehd2a Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-zDicer
Plasmid#171539PurposeEncodes Zebrafish Dicer proteinDepositorInsertdicer1(MK642699) (dicer1 Zebrafish)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
M16 pCS-twhh-bGFP
Plasmid#17153DepositorInserttwhh promoter (shhb Zebrafish)
UseTagsbGFPExpressionMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
M17 ptwhh-IGFP65C
Plasmid#17154DepositorInserttwhh promoter (shhb Zebrafish)
UseTagsb-globin intron, GFPExpressionMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pT2KXIG (Tol2)-tp63:ikk1C179A:GFP
Plasmid#105647PurposeExpression of Ikk1(alpha) with mutated cysteine residues at position 179 in basal keratinocytesDepositorInserttp63:ikk1C179A:GFP (chuk Zebrafish)
UseTagsGFPExpressionMammalianMutationp63 promoter + ikk1C179A + GFPPromoterAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only