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Plasmid#125645Purposemicro-injection of poly-adenylated Hypa-nCas9n RNADepositorInsertHypa-nCas9n
UseRna in vitro transcription vectorTagsExpressionMutationN692A, M694A, Q695A, H698A from wild-type Cas9PromoterT3Available sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRII HSPB8
Plasmid#61035PurposeProbe for HSPB8 for in situ hybridization in zebrafishDepositorInsertHSPB8 (hspb8 Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
ZE44 myc-Dapper-2 pCS2P+
Plasmid#17085DepositorInsertDapper-2 (dact2 Zebrafish)
UseZebrafish expressionTagsmycExpressionMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCSDest
Plasmid#22423DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceDec. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HMGN1
Plasmid#192718PurposeGateway entry vector encoding human HMGN1DepositorInsertHMGN1 (HMGN1 Human)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2
Plasmid#22424DepositorTypeEmpty backboneUseDestination vectorTagsExpressionMutationPromoterAvailable sinceDec. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
ZP17 Krox20
Plasmid#16924DepositorInsertKrox20 (egr2b Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pTol2-HuC(elavl3)-CaMPARI2
Plasmid#137185Purposepan-neuronal expression of CaMPARI2 in zebrafish, used to generate the CaMPARI2 zebrafish line at ZIRC (ZL13801)DepositorInsertHuC(elavl3)-CaMPARI2-FLAG-HA-myc-polyA
UseTol2 plasmid for zebrafishTagsFLAG, HA, mycExpressionMutationPromoterHuC(elavl3)Available sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-zSpG
Plasmid#179316PurposeIn vitro transcription of SpG-Cas9 mRNA zebrafish codon-optimized from T3 promoterDepositorInsertZebrafish codon-optimized Cas9 variant SpG
UseCRISPR; In vitro transcription by t3 promoterTagsSV40-NLSExpressionMutationSpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337RPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSYC-437
Plasmid#163829PurposeFoxj1a:GCaMP6s. Fluorescent reporter for calcium signaling (GCaMP6s) driven by zebrafish foxj1a promoter.DepositorInsertsFoxj1a Promoter
GCaMP6s
UseMultisite gateway destination vectorTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-zSpRY
Plasmid#179317PurposeIn vitro transcription of SpRY-Cas9 mRNA zebrafish codon-optimized from T3 promoterDepositorInsertZebrafish codon-optimized Cas9 variant SpRY
UseCRISPR; In vitro transcription by t3 promoterTagsSV40-NLSExpressionMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…PromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
p5E-EF1a/b-actin
Plasmid#82583Purpose5' entry vector containing the EF1a/B-globin fused to zebrafish b-actin 2 enhancer/promoter for strong semi-ubiquitous expressionDepositorInsertEF1a/b-globin fused to zebrafish b-actin 2 enhancer/promoter
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-HuC:Dendra2
Plasmid#80904PurposeInjection into Zebrafish larvae together with Tol2-Transposase to achieve pan-neuronal expression of the photoconvertible fluorescent protein Dendra2DepositorInsertDendra2 (elavl3 Synthetic)
UseZebrafish expressionTagsExpressionMutationPromoterHuCAvailable sinceAug. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS U6a gRNA
Plasmid#158961PurposegRNA backbone driven by zebrafish U6a promoterDepositorInsertszf U6a promoter
gRNA backbone
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Cas9
Plasmid#47322PurposeFor in vivo expression of Cas9 or generation of Cas9 mRNADepositorInsertCas9
UseCRISPR; Xenopus and zebrafish expressionTagsExpressionMammalianMutationPromoterSP6Available sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
Zebrabow-GateDest
Plasmid#118221PurposeFor cloning promoter into a Zebrabow vector with Gateway reaction. Only compatible with single site Gateway. Not compatible with Tol2-kit.DepositorInsertBrainbow 1.0 L - ccdB survival cassette
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVC-Tol2-Pax3a_enh5-E1b:eGFP-Tol2
Plasmid#119061PurposeFluorescent vector with zebrafish pax3a enhancer 5; Tol2 versionDepositorInsertpax3a_enh5-E1b:eGFP
UseTagsExpressionBacterialMutationPromoterE1b minimal promoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHis-zfEpoa
Plasmid#65611PurposeExpress soluble His-tagged zebrafish erythropoietin in E. coliDepositorInserterythropoietin (epoa Zebrafish)
UseTags(His)6ExpressionBacterialMutationPromoterT7Available sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2+PIF6CreN
Plasmid#131780PurposeMammalian, Zebrafish, or Xenopus expression of PIF6CreN, one half of the CreLite systemDepositorInsertPIF6CreN
UseCre/Lox; Zebrafish expressionTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only