-
Plasmid#55221DepositorTypeEmpty backboneUseTagsStrepII and msfGFPExpressionInsectMutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only
-
pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V1)
Plasmid#55222DepositorTypeEmpty backboneUseTagsSNAPf and TwinStrepIIExpressionInsectMutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V3)
Plasmid#55223DepositorTypeEmpty backboneUseTagsSNAPf and TwinStrepIIExpressionInsectMutationPromoterAvailable sinceJuly 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)
Plasmid#217808PurposeExpression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)DepositorInsert5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); EcKlenow(-exo;D355A/E357A)PromoterT7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
Click editor utilizing Cdc13(Y556A) for template recruitment - pCMV-T7-Cdc13(Y556A)-nCas9-EcKlenow (JO1329)
Plasmid#217797PurposeVariant CE1 construct with Cdc13(Y556A) telomere binding protein for template recruitment, expressed from CMV or T7 promoters.DepositorInsertCdc13(Y556A)-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationCdc13(Y556A); nSpCas9(H840A); EcKlenow(-exo;D355A…PromoterCMV and T7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
Click editor utilizing mSA for template recruitment and ePhi29 DNA polymerase - pCMV-T7-mSA-nCas9-ePhi29 (JO1398)
Plasmid#217803PurposeVariant CE1 construct with mSA for template recruitment and ePhi29(D169A) DNA pol, expressed from CMV or T7 promoters.DepositorInsertmSA-linker-SV40NLS-nSpCas9-BPNLS-ePhi29-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); Phi29(D169A/M8R/V51A/M97T/G197D/E…PromoterCMV and T7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
SPOCK2_22-424_AviN_HisC
Plasmid#195877PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertSPOCK2 (SPOCK2 Human)
UsePfhmsp-avin-lic-c, baculovirus expressionTagsExpressionMutationPromoterPolyhedrinAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
NXPH1_22-271_AviN_HisC
Plasmid#195872PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertNXPH1 (NXPH1 Human)
UsePfhmsp-avin-lic-c, baculovirus expressionTagsExpressionMutationPromoterPolyhedrinAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
SPOCK1_23-439_HisN_AviC
Plasmid#195876PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertSPOCK1 (SPOCK1 Human)
UsePfhmsp-avic-lic-n, baculovirus expressionTagsExpressionMutationPromoterPolyhedrinAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
OLFML3_123-406_AviN_HisC
Plasmid#195871PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertOLFML3 (OLFML3 Human)
UsePfhmsp-avin-lic-c, baculovirus expressionTagsExpressionMutationPromoterPolyhedrinAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
NTN1_39-453_AviN_HisC
Plasmid#195873PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertNTN1 (NTN1 Human)
UsePfhmsp-avin-lic-c, baculovirus expressionTagsExpressionMutationPromoterPolyhedrinAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
COL11A1_36-250_HisN_AviC
Plasmid#195878PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertCOL11A1 (COL11A1 Human)
UsePfhmsp-avic-lic-n, baculovirus expressionTagsExpressionMutationPromoterPolyhedrinAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
COL11A1_1570-1806_HisN_AviC
Plasmid#195879PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertCOL11A1 (COL11A1 Human)
UsePfhmsp-avic-lic-n, baculovirus expressionTagsExpressionMutationPromoterPolyhedrinAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
ECE1_101-770_HisN_AviC
Plasmid#195875PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertECE1 (ECE1 Human)
UsePfhmsp-avic-lic-n, baculovirus expressionTagsExpressionMutationPromoterPolyhedrinAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
COL25A1_53-654_AviN_HisC
Plasmid#195880PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertCOL25A1 (COL25A1 Human)
UsePfhmsp-avin-lic-c, baculovirus expressionTagsExpressionMutationPromoterPolyhedrinAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
8VIS
Plasmid#220881PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertTMPRSS11D (TMPRSS11D Human)
UseBaculovirus expresssionTagsEFVEHHHHHHHHExpressionMutationPromoterPolyhedrinAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
avi-eTMPRSS11D S368A
Plasmid#220882PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertTMPRSS11D (TMPRSS11D Human)
UseBaculovirus expresssionTagsHoneybee melittinsignalExpressionMutationS368APromoterPolyhedrinAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
8V04
Plasmid#220879PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertTMPRSS2 (TMPRSS2 Human)
UseBaculovirus expresssionTagsEFVEHHHHHHHH and Honeybee melittin signalExpressionMutationS251D/R252D/Q253D/S254D/R255KPromoterPolyhedrinAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET30Xa-SBL1(160S)
Plasmid#66872PurposeExpresses WTsoybean lipoxygenase-1 160S with N-term HistagDepositorInsertsoybean lipoxygenase-1 wild type (NEWENTRY Glycine max)
UseTagsHistag from pET30Xa-LICExpressionBacterialMutationnonePromoterT7lacAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
3D8H
Plasmid#51332DepositorInsert3D8H
UseTagsHisExpressionBacterialMutationTruncation: See CommentsPromoterT7Available sinceFeb. 12, 2014AvailabilityAcademic Institutions and Nonprofits only