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Showing: 441 - 460 of 11872 results

  4. Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)

    Plasmid
    #217808
    Purpose
    Expression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217808-plasmid-map-sequence-id-432916

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  5. Click editor utilizing Cdc13(Y556A) for template recruitment - pCMV-T7-Cdc13(Y556A)-nCas9-EcKlenow (JO1329)

    Plasmid
    #217797
    Purpose
    Variant CE1 construct with Cdc13(Y556A) telomere binding protein for template recruitment, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    Cdc13(Y556A)-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Cdc13(Y556A); nSpCas9(H840A); EcKlenow(-exo;D355A…
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217797-plasmid-map-sequence-id-432864

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  6. Click editor utilizing mSA for template recruitment and ePhi29 DNA polymerase - pCMV-T7-mSA-nCas9-ePhi29 (JO1398)

    Plasmid
    #217803
    Purpose
    Variant CE1 construct with mSA for template recruitment and ePhi29(D169A) DNA pol, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    mSA-linker-SV40NLS-nSpCas9-BPNLS-ePhi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); Phi29(D169A/M8R/V51A/M97T/G197D/E…
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217803-plasmid-map-sequence-id-432890

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  7. SPOCK2_22-424_AviN_HisC

    Plasmid
    #195877
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SPOCK2 (SPOCK2 Human)
    Use
    Pfhmsp-avin-lic-c, baculovirus expression
    Tags
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195877-plasmid-map-sequence-id-387267

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  8. NXPH1_22-271_AviN_HisC

    Plasmid
    #195872
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NXPH1 (NXPH1 Human)
    Use
    Pfhmsp-avin-lic-c, baculovirus expression
    Tags
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195872-plasmid-map-sequence-id-387247

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  9. SPOCK1_23-439_HisN_AviC

    Plasmid
    #195876
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SPOCK1 (SPOCK1 Human)
    Use
    Pfhmsp-avic-lic-n, baculovirus expression
    Tags
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195876-plasmid-map-sequence-id-387253

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  10. OLFML3_123-406_AviN_HisC

    Plasmid
    #195871
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    OLFML3 (OLFML3 Human)
    Use
    Pfhmsp-avin-lic-c, baculovirus expression
    Tags
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195871-plasmid-map-sequence-id-397083

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  11. NTN1_39-453_AviN_HisC

    Plasmid
    #195873
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NTN1 (NTN1 Human)
    Use
    Pfhmsp-avin-lic-c, baculovirus expression
    Tags
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195873-plasmid-map-sequence-id-387265

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  12. COL11A1_36-250_HisN_AviC

    Plasmid
    #195878
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    COL11A1 (COL11A1 Human)
    Use
    Pfhmsp-avic-lic-n, baculovirus expression
    Tags
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195878-plasmid-map-sequence-id-387254

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  13. COL11A1_1570-1806_HisN_AviC

    Plasmid
    #195879
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    COL11A1 (COL11A1 Human)
    Use
    Pfhmsp-avic-lic-n, baculovirus expression
    Tags
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195879-plasmid-map-sequence-id-387249

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  14. ECE1_101-770_HisN_AviC

    Plasmid
    #195875
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ECE1 (ECE1 Human)
    Use
    Pfhmsp-avic-lic-n, baculovirus expression
    Tags
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195875-plasmid-map-sequence-id-387251

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  15. COL25A1_53-654_AviN_HisC

    Plasmid
    #195880
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    COL25A1 (COL25A1 Human)
    Use
    Pfhmsp-avin-lic-c, baculovirus expression
    Tags
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195880-plasmid-map-sequence-id-387264

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  16. 8VIS

    Plasmid
    #220881
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TMPRSS11D (TMPRSS11D Human)
    Use
    Baculovirus expresssion
    Tags
    EFVEHHHHHHHH
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    Sept. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    220881-plasmid-map-sequence-id-436700

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  17. avi-eTMPRSS11D S368A

    Plasmid
    #220882
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TMPRSS11D (TMPRSS11D Human)
    Use
    Baculovirus expresssion
    Tags
    Honeybee melittinsignal
    Expression
    Mutation
    S368A
    Promoter
    Polyhedrin
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    220882-plasmid-map-sequence-id-436699

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  18. 8V04

    Plasmid
    #220879
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TMPRSS2 (TMPRSS2 Human)
    Use
    Baculovirus expresssion
    Tags
    EFVEHHHHHHHH and Honeybee melittin signal
    Expression
    Mutation
    S251D/R252D/Q253D/S254D/R255K
    Promoter
    Polyhedrin
    Available since
    Sept. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    220879-plasmid-map-sequence-id-436702

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  20. 3D8H

    Plasmid
    #51332
    Depositor
    Cheryl Arrowsmith
    Insert
    3D8H
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available since
    Feb. 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    51332-plasmid-map-sequence-id-338743

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Showing: 441 - 460 of 11872 results