NXPH1_22-271_AviN_HisC
(Plasmid
#195872)
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PurposeBaculovirus expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 195872 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepFHMSP-AviN-LIC-C
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Backbone manufacturerpFHMSP-LIC-C (Plasmid #26100)
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Vector typepFHMSP-AviN-LIC-C, Baculovirus Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNXPH1
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SpeciesH. sapiens (human)
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Insert Size (bp)750
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Entrez GeneNXPH1 (a.k.a. NPH1, Nbla00697)
- Promoter Polyhedrin
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer pFBOH-fwd (5'-CCGGATTATTCATACCGTCCCACCA-3')
- 3′ sequencing primer pFBOH-rev (5'-CTGATTATGATCCTCTAGTACTTCT-3') (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
SGC structures for NIH Treat-AD Project
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NXPH1_22-271_AviN_HisC was a gift from Cheryl Arrowsmith (Addgene plasmid # 195872 ; http://n2t.net/addgene:195872 ; RRID:Addgene_195872)
