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JC172

Plasmid

#104948

Purpose

Methanol inducing HSA-hGH fusion protein, and estrogen inducing TEV protease

Depositor

Timothy Lu

Article

Cao et al Nat Commun. 2018 Jan 8;9(1):77. doi: 10.

Insert

HSA-hGH fusion protein and TEV protease

Use

Tags

Expression

Mutation

Promoter

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB2223

Plasmid

#67544

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae X-3 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pDS1U-PKS12

Plasmid

#70150

Purpose

Fusarium graminearum PKS12 encoded on CEN/ARS yeast shuttle vector with DS1U selection marker

Depositor

Morten Sommer

Article

Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

Insert

PKS12 (PKS12 Fusarium graminearum)

Use

Tags

Expression

Yeast

Mutation

Promoter

CUP1

Available since

Dec. 23, 2015

Availability

Academic Institutions and Nonprofits only

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JC031

Plasmid

#104953

Purpose

Methanol inducing hGH, estrogen inducing G-CSF, and IPTG inducing Interferon

Depositor

Timothy Lu

Article

Cao et al Nat Commun. 2018 Jan 8;9(1):77. doi: 10.

Insert

hGH, Interferon and G-CSF

Use

Tags

Expression

Mutation

Promoter

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pAAH3

Plasmid

#41827

Purpose

Used as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for phleomycin resistance.

Depositor

Melissa Moore

Article

Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

Type

Empty backbone

Use

Pcr template

Tags

Expression

Yeast

Mutation

Promoter

TEF

Available since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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p413GPD-hTPI Ile170Thr

Plasmid

#50721

Purpose

expression of the human TPI Ile170Thr allele in yeast

Depositor

Markus Ralser

Article

Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

Insert

Triosephosphate isomerase 1 (TPI1 Human)

Use

Tags

Expression

Yeast

Mutation

Isoleucine 170 to Threonine

Promoter

GPD

Available since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only

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YIp211-P30-His-pol30(K127R)-3'

Plasmid

#99547

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127R; URA3 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

K127R

Promoter

POL30

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pJRL2 PHO5(TATA-A1T6)pr ECFP (EB#1560)

Plasmid

#14846

Depositor

Erin O'Shea

Article

Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

Insert

PHO5 promoter TATA-A1T6 (PHO5 Budding Yeast)

Use

Tags

ECFP

Expression

Yeast

Mutation

TATA-A1T6. TATA box mutated from TATATAAG to AATA…

Promoter

Available since

May 25, 2007

Availability

Academic Institutions and Nonprofits only
pJRL2 PHO5(UASm1)pr VYFP (EB#1549)

Plasmid

#14841

Depositor

Erin O'Shea

Article

Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

Insert

PHO5 promoter UASm1 (PHO5 Budding Yeast)

Use

Tags

VYFP

Expression

Yeast

Mutation

UASm1 (AAGCTT was substituted for CACGTT). Unindu…

Promoter

Available since

May 25, 2007

Availability

Academic Institutions and Nonprofits only
pJRL2 PHO5(TATA-C2)pr ECFP (EB#1562)

Plasmid

#14844

Depositor

Erin O'Shea

Article

Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

Insert

PHO5 promoter TATA-C2 (PHO5 Budding Yeast)

Use

Tags

ECFP

Expression

Yeast

Mutation

TATA-C2. TATA box mutated from TATATAAG to TCTA…

Promoter

Available since

May 25, 2007

Availability

Academic Institutions and Nonprofits only
pT-36

Plasmid

#16252

Depositor

Yoshiko Kikuchi

Article

Takahashi et al J Biol Chem. 2001 Dec 28. 276(52):48973-

Insert

CDC3 (CDC3 Budding Yeast)

Use

Tags

His and T7

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
300_pETcon_SARS2_FLip

Plasmid

#222230

Purpose

yeast surface display of the SARS-CoV-2 FLip variant RBD

Depositor

Tyler Starr

Article

Taylor et al Virus Evolution 2024

Insert

SARS-CoV-2 FLip RBD (S SARS-CoV-2 virus, Budding Yeast)

Use

Tags

HA and c-Myc

Expression

Yeast

Mutation

Promoter

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-Q443P

Plasmid

#216401

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Q443 to proline mutation

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-ΔDBD-Q443P

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Glutamine 443 to Proline, and deleted ami…

Promoter

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pXOOY

Plasmid

#196449

Purpose

A dual vector for expression of membrane proteins in S. cerevisiae and X. laevis

Depositor

Per Amstrup Pedersen

Article

Vold et al PLoS One. 2023 Feb 21;18(2):e0281868. do

Type

Empty backbone

Use

Expression in xenopus laevis

Tags

Expression

Yeast

Mutation

Promoter

yeast CYC-GAL and T7 promoter

Available since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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RPO41 deltaN100

Plasmid

#205073

Purpose

RPO41 protein expression

Depositor

Smita Patel

Article

Paratkar et al J Biol Chem. 2011 May 6;286(18):16109-20

Insert

RPO41 (RPO41 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Lacks N Terminal 100 amino acids

Promoter

Available since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

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RPO41 deltaN380

Plasmid

#205076

Purpose

RPO41 protein expression

Depositor

Smita Patel

Article

Paratkar et al J Biol Chem. 2011 May 6;286(18):16109-20

Insert

RPO41 (RPO41 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Lacks N Terminal 380 amino acids

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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RPO41 deltaN296

Plasmid

#205075

Purpose

RPO41 protein expression

Depositor

Smita Patel

Article

Paratkar et al J Biol Chem. 2011 May 6;286(18):16109-20

Insert

RPO41 (RPO41 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Lacks N Terminal 296 amino acids

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pYP-ptef1-GFP

Plasmid

#199990

Purpose

Yeast/E coli shuttle vector expressing GFP from tef1 promoter

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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Edc3

Plasmid

#201413

Purpose

Bacterial protein expression of Edc3

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Edc3 (EDC3 Budding Yeast)

Use

Tags

MBP, TEV and TEV, 6xHis

Expression

Bacterial

Mutation

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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Pat1

Plasmid

#201414

Purpose

Bacterial protein expression of Pat1

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Pat1 (PAT1 Budding Yeast)

Use

Tags

MBP, TEV and TEV, 6xHis

Expression

Bacterial

Mutation

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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