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pCAMKIIa-TetOn3G

Plasmid

#133727

Purpose

Plasmid contains the TetOn3G-transactivator sequence under control of a Camk2a promoter.

Depositor

Erin Schuman

Article

Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Insert

TetOn3G

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

pCamk2a

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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Syn:eGFP-CaM-uTEV1Δ(220-242)

Plasmid

#135463

Purpose

Neuronal expression of uFLARE protease component

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

eGFP-CaM-uTEV1Δ

Use

AAV

Tags

eGFP, V5

Expression

Mammalian

Mutation

S219V mutation improves stability and S153N impro…

Promoter

Synapsin

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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JD182

Plasmid

#229488

Purpose

Ef1a promoter driving expression of adenoviral L4-22k gene

Depositor

Nerea Zabaleta

Article

Doshi et al Molecular Therapy Methods & Clinical Dev

Insert

L4-22k

Use

AAV and Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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JD136

Plasmid

#229497

Purpose

Adenoviral helper plasmid containing E2A and L4-22k/33k and VA RNA I genes

Depositor

Nerea Zabaleta

Article

Doshi et al Molecular Therapy Methods & Clinical Dev

Insert

E2A, L4-22k/33k, VA RNA I

Use

AAV and Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

wt promoter

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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JD183

Plasmid

#229489

Purpose

Ef1a promoter driving expression of adenoviral L4-33k gene

Depositor

Nerea Zabaleta

Article

Doshi et al Molecular Therapy Methods & Clinical Dev

Insert

L4-33k

Use

AAV and Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pProk2.Cre.T2A.mCherry

Plasmid

#169013

Purpose

Expresses Cre under Prok2 minimal promoter in mammalian cells

Depositor

Michael Hastings

Insert

Cre Recombinase

Use

AAV and Cre/Lox

Tags

T2A and mCherry

Expression

Mammalian

Mutation

Promoter

Prokineticin 2

Available since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pProkR2.Cre.T2A.Venus

Plasmid

#169014

Purpose

Expresses Cre under Prok receptor 2 minimal promoter in mammalian cells

Depositor

Michael Hastings

Insert

Cre Recombinase

Use

AAV and Cre/Lox

Tags

T2A and Venus

Expression

Mammalian

Mutation

Promoter

Prokineticin Receptor 2

Available since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pAM/SAR-CAG-WPRE-bGHpA

Plasmid

#194243

Purpose

empty backbone

Depositor

Michael J Fox Foundation MJFF

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Jan. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Olig001

Plasmid

#170716

Purpose

Olig001 encodes a novel AAV capsid that exhibits preferential oligodendrocyte tropism in the CNS

Depositor

Thomas McCown

Article

Powell et al Gene Ther. 2016 Nov;23(11):807-814. doi:

Insert

Olig001 capsid gene / rep 2

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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mtagBFP2-CaM-uTEVp

Plasmid

#163033

Purpose

FLiCRE protease for neuronal expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

BFP-CaM-uTEVp

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEMS1982

Plasmid

#49113

Purpose

AAV plasmid with CLDN5 (Ple261) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Baumann et al Exp Eye Res. 2019 Jul 16:107728. doi: 10

Insert

ssAAV-Ple261-iCre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CLDN5

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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TRE:mCherry-p2a- bReaChES

Plasmid

#163037

Purpose

FLiCRE reporter gene for neuronal expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

mCherry-p2a-bReaChES

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-CaM-uTEVp

Plasmid

#163032

Purpose

FLiCRE protease for neuronal expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

GFP-CaM-uTEVp

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-GFP-CaM-uTEVp

Plasmid

#163028

Purpose

FLiCRE protease for HEK293T expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

GFP-CaM-uTEVp

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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Ca-FLARE (protease)

Plasmid

#92214

Purpose

protease component of FLARE

Depositor

Alice Ting

Article

Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

CaM-V5-TEVp(Δ220-242)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

synapsin

Available since

June 16, 2017

Availability

Academic Institutions and Nonprofits only

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pEMS2118

Plasmid

#49143

Purpose

AAV plasmid with smCBA promoter driving expression of iCre. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Mirjalili Mohanna et al Gene Ther. 2023 Apr 19. doi: 10.1038/s41

Insert

ssAAV-smCBA-iCre-WPRE

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

smCBA

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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JD180

Plasmid

#229486

Purpose

Adenoviral helper plasmid containing inducible E2A and L4-22k/33k genes

Depositor

Nerea Zabaleta

Article

Doshi et al Molecular Therapy Methods & Clinical Dev

Insert

E2A, L4-22k/33k, mCitrine

Use

AAV and Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

Tre (E2A, L4-22k/33k), ETR (mCitrine)

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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hSyn-DIO-somBiPOLES-mCerulean

Plasmid

#154951

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Has Service

AAV Retrograde, AAV5, and AAV9

Insert

somBiPOLES

Use

AAV

Tags

soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Mutation

Promoter

human synapsin

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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CaMKII-somBiPOLES-mCerulean

Plasmid

#154948

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Has Service

AAV Retrograde, AAV5, and AAV9

Insert

somBiPOLES

Use

AAV

Tags

soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Mutation

Promoter

CaMKIIa(0.4)

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 with interspersed loops (GAPDH)

Plasmid

#180183

Purpose

AAV vector carrying a guide RNA targeting the human GAPDH mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA with interspersed loops (GAPDH Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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