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Plasmid#80921PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only
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All-in-one lenti-ABE8e-NG
Plasmid#200447PurposeLentivirus vector for base editingDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBinit
Plasmid#110100PurposeE.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal myc and 6xHisTagDepositorTypeEmpty backboneUseTagsmyc, HisExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO GFP-MDC1 (779-2)
Plasmid#26285DepositorInsertMediator of DNA damage checkpoint 1 (MDC1 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO-H2B-SunTag24x_v1
Plasmid#60911PurposeEnocdes a histone H2B fused to the SunTag for single molecule imagingDepositorInsertH2B
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSUMO1
Plasmid#52258PurposeBacterial expression of human 6His-tagged SUMO1, SUMO-E1 and SUMO-E2DepositorUseTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
HPV16 E7 TCR
Plasmid#122728PurposeExpress HPV16 E7 TCRDepositorInsertHPV16 E7 TCR
UseRetroviralTagsExpressionMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGD-P1/HC-Pro
Plasmid#196328Purpose35S promoter-driven expression of the tobacco etch virus P1/HC-Pro RNA silencing suppressorDepositorInserttobacco etch virus P1/HC-Pro
UseTagsExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTALdNC-AAVS1_T1
Plasmid#80495PurposeAAVS1 TALEN expression vector (Left)DepositorInsertAAVS1 TALEN (Left)
UseGateway entryTagsExpressionMutationPromoternoneAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTALdNC-AAVS1_T2
Plasmid#80496PurposeAAVS1 TALEN expression vector (Right)DepositorInsertAAVS1 TALEN (Right)
UseGateway entryTagsExpressionMutationPromoternoneAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-Ost1-alphaf(I)-E2-Crimson
Plasmid#117661PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpre-Ost1-alphaf(I)-E2-Crimson
UseTagsExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-CjABE8e
Plasmid#189929PurposePlasmid encoding CjABE8e driven by the CMV promoter for plasmid transfection.DepositorInsertCjABE8e
UseTagsExpressionMammalianMutationCjCas9 D8APromoterCMVAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBad-EBFP2
Plasmid#14891PurposeBacterial expression of EBFP2 blue fluorescent proteinDepositorHas ServiceCloning Grade DNAInsertEBFP2
UseTagsHisExpressionBacterialMutationThe fluorescent protein was inserted between XhoI…PromoterAvailable sinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FAH-rtTA3G
Plasmid#120309PurposeAn AAV vector that expresses rtTA3G under the c-fos promoterDepositorInsertrtTA3G
UseAAVTagsExpressionMutationPromoterc-fosAvailable sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
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ptf-Galectin3
Plasmid#64149PurposeExpression in mammalian cellsDepositorInsertGalectin3 (LGALS3 Human)
UseTagsEGFP and mRFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
p414-TEF1p-Cas9-CYC1t
Plasmid#43802DepositorArticleInsertHuman Optimized S. pyogenes Cas9
UseCRISPRTagsExpressionYeastMutationPromoterTEF1 promoterAvailable sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEDA5_GFPmut3_Y66H
Plasmid#80085PurposePositive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.DepositorInsertGFPmut3_Y66H
UseTags6x-His tagExpressionBacterialMutationchanged Tyrosine 66 to HistidinePromoterproBAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 TFAM-mClover
Plasmid#129574PurposeExpression of mClover fused TFAMDepositorInsertTFAM (TFAM Human)
UseTagsExpressionMammalianMutationNonePromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSFFV-EGFP-IRES2-mCherry (FACS reporter)
Plasmid#222999PurposeFACS reporter constitutively expressing mCherry and conditionally expressing EGFP upon prime editing. For lentivirus packaging.DepositorInsertEGFP-IRES2-mCherry
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
dSV40-NLS-dCas9-HA-NLS-NLS-10xGCN4
Plasmid#107310PurposeEncoding dCas9-10xGCN4 driven by dSV40 promoterDepositorInsertdSV40-dCas9-10xGCN4
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_SARS2_JN.1
Plasmid#218678Purposeexpressing SARS-CoV-2 JN.1 spike protein for pseudovirus productionDepositorInsertSpike (JN.1)
UseTagsExpressionMammalianMutationL455S on the basis of BA.2.86PromoterCMVAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)
Plasmid#196702PurposePlasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.DepositorInserthuman RNaseH1 wild type
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC1-SypHer3s-IMS
Plasmid#108120PurposeMitochondrial ratiometric pH probe with enhanced brightness fused to Smac/DIABLO fragment. Probe is localized in mitochondrial intermembrane space. Mammalian cell expression.DepositorInsertSypHer3s-IMS
UseTagsratiometricYFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-OTUB1
Plasmid#22551DepositorInsertOTUB1 (OTUB1 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pIG-641 JE-TRAC-BCMA(BB)28z-P2A
Plasmid#186127PurposeBCMA-CAR DNA productionDepositorInsertBCMA-CAR (TNFRSF17 Human)
UseCRISPRTagsExpressionMutationWTPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV- δLight
Plasmid#201224PurposeExpress deltaLight biosensor in mammalian cellsDepositorInsertδLight1.3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC25A44
Plasmid#132042PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC25A44 (SLC25A44 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1
Plasmid#213025PurposeExpresses full length human LINE-1 ORF2p Core (1-1275) with a C-terminal 3xFlag tag for baculovirus production in insect cellsDepositorInsertLINE-1 ORF2p (1-1275)
UseTags3C-3xFlag (ORF2p)ExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W
Plasmid#67986PurposeCas9 activity reporter with mCherry and BFPDepositorInsertsU6gRNA cassette, PGKmCherryABFP cassette, WPRE
Guide RNA targeting modified BFP
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPRE, modified BFPPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only