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Plasmid#79707PurposeThis plasmid encodes the kinase domain of CSK21. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertCSK21
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
RGD-ACP-I27-CC
Plasmid#85391PurposeMTFM sensor to measure integrin mechanical forces. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines (CC) for immobilization and ACP tag for fluorophore labelingDepositorInsertRGD motif for integrin binding fused to acyl carrier protein (ACP) tag and titin immunoglobulin domain (I27)
UseTags6x HisExpressionBacterialMutationPromoterT7Available sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
KAPCA_HUMAN_D0
Plasmid#79741PurposeThis plasmid encodes the kinase domain of KAPCA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKAPCA
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pACB108
Plasmid#222207PurposeFor recombinant expression of fghA (PP_1617 from Pseudomonas putida) with a C-terminal His tagDepositorInsertfghA
UseTags6x HisExpressionBacterialMutationPromoterT7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACB109
Plasmid#222208PurposeFor recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the K10N mutation and a C-terminal His tagDepositorInsertfghA (K10N)
UseTags6x HisExpressionBacterialMutationChanged Lys10 to AsnPromoterT7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACB110
Plasmid#222209PurposeFor recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G258D mutation and a C-terminal His tagDepositorInsertfghA (G258D)
UseTags6x HisExpressionBacterialMutationChanged Gly258 to AspartatePromoterT7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB1-A11(2-88)-Strep
Plasmid#196214PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-88)-Strep.DepositorInsertAnnexin A11 (ANXA11 Human)
UseTagsGB1-6xHis-TEV and Strep-II tagExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-A11(89-196)
Plasmid#196215PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(89-196).DepositorInsertAnnexin A11 (ANXA11 Human)
UseTagsGB1-6xHis-TEV and Strep-II tagExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-A11(2-68)-Strep
Plasmid#196216PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-68)-Strep.DepositorInsertAnnexin A11 (ANXA11 Human)
UseTagsGB1-6xHis-TEV and Strep-II tagExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-A11(2-196)-Strep
Plasmid#196208PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196)-Strep.DepositorInsertAnnexin A11 (ANXA11 Human)
UseTagsGB1-6xHis-TEV and Strep-II tagExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-A11(2-52)-Strep
Plasmid#196213PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-52)-Strep.DepositorInsertAnnexin A11 (ANXA11 Human)
UseTagsGB1-6xHis-TEV and Strep-II tagExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-ΔN-CHMP4C(G154C)
Plasmid#190783PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, G154C)DepositorInsertGB1-His6-TEV-CHMP4C(121-233, G154C) (CHMP4C Human)
UseTagsGB1-His6-TEVExpressionBacterialMutationG154CPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-ΔN-CHMP4C(M165C)
Plasmid#190784PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, M165C)DepositorInsertGB1-His6-TEV-CHMP4C(121-233, M165C) (CHMP4C Human)
UseTagsGB1-His6-TEVExpressionBacterialMutationM165CPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-ΔN-CHMP4C(S191C)
Plasmid#180027PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, S191C).DepositorInsertGB1-His6-TEV-CHMP4C(121-233, S191C) (CHMP4C Human)
UseTagsGB1-His6-TEVExpressionBacterialMutationS191CPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBtPIPLC_N168C His
Plasmid#173809PurposeExpression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cellsDepositorInsertBacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
UseTags6XHisExpressionBacterialMutationAsn168Cys for labelingPromoterT7Available sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3R112C/T180CHFm
Plasmid#85091PurposeExpresses PCNA3 variant (R112C/T180C) fused to heme-FMN domain of P450 BM3 variant (A74G) in E. coliDepositorInsertThe R112C/T180C variant of PCNA3 fused to Heme-FMN domain of P450 BM3
UseTagsHis6 tagExpressionBacterialMutationR112C/T180C in PCNA3, A74G in heme-FMN domain of …PromoterT7 promoterAvailable sinceDec. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
HASP_HUMAN_D0
Plasmid#79730PurposeThis plasmid encodes the kinase domain of HASP. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertHASP
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
KCC2D_HUMAN_D0
Plasmid#79734PurposeThis plasmid encodes the kinase domain of KCC2D. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKCC2D
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAPK2_HUMAN_D0
Plasmid#79738PurposeThis plasmid encodes the kinase domain of AAPK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertAAPK2
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
KCC4_HUMAN_D0
Plasmid#79744PurposeThis plasmid encodes the kinase domain of KCC4. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKCC4 (SLC12A7 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only