GB1-ΔN-CHMP4C(S191C)
(Plasmid
#180027)
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PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, S191C).
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 180027 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET-11a
- Backbone size w/o insert (bp) 5393
- Total vector size (bp) 6264
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGB1-His6-TEV-CHMP4C(121-233, S191C)
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Alt nameCHMP4C
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Alt nameCharged multivesicular body protein 4c
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SpeciesH. sapiens (human)
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Insert Size (bp)621
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MutationS191C
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GenBank ID
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Entrez GeneCHMP4C (a.k.a. SNF7-3, Shax3, VPS32C)
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Tag
/ Fusion Protein
- GB1-His6-TEV (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GB1-ΔN-CHMP4C(S191C) was a gift from Lalit Deshmukh (Addgene plasmid # 180027 ; http://n2t.net/addgene:180027 ; RRID:Addgene_180027) -
For your References section:
Reversible phase separation of ESCRT protein ALIX through tyrosine phosphorylation. Elias RD, Zhu Y, Su Q, Ghirlando R, Zhang J, Deshmukh L. Sci Adv. 2023 Jul 14;9(28):eadg3913. doi: 10.1126/sciadv.adg3913. Epub 2023 Jul 14. 10.1126/sciadv.adg3913 PubMed 37450591
