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Plasmid#157897PurposeExpresses the AVAST type 3 defense systemDepositorInsertAVAST type 3 (STAND ATPase with N-terminal DUF4297)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ906
Plasmid#44472DepositorInsertMinimal Mos1, unc-119, [4-3] Gateway
UseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO DEST (775-1)
Plasmid#17432Purpose3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoterDepositorTypeEmpty backboneUseLentiviral; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCK364.306
Plasmid#192642PurposeExpresses dSpRY and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmRDepositorInsertJ306 scRNA
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterBBa_J23119Available sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03
Plasmid#67137PurposeGateway destination vector for cell-free expression with N-term 8xHis eGFP tagDepositorTypeEmpty backboneUseCell-free expressionTagsEGFP and HisExpressionMutationPromoterAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJRA108
Plasmid#209341PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mCherry fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNLuc
Plasmid#118058PurposeGBPart - NLuc Luciferase CDSDepositorInsertNano Luciferase
UseLuciferase and Synthetic Biology; GbpartTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB105
Plasmid#68559PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsCitrineExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJRA107
Plasmid#209340PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mNeonGreen fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAC-BA-lacZ
Plasmid#13423DepositorInsertBA
UseCre/LoxTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)::NL3F10H
Plasmid#141290PurposeNLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertNLUC3F10H
UseLuciferaseTagsFlag (x3) and His (x10)ExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB108
Plasmid#68562PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsCitrineExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
PylRS-AS
Plasmid#137908PurposeFor incorporation of fluorophenylalanine derivativesDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
UseTagsExpressionBacterialMutationContaining N346A, C348S mutationsPromoterpBADAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2xp53_RE
Plasmid#118053PurposeGBPart - Operator/DNA Response element - 2 copies of the p53 DNA binding motifDepositorInsert2 copies of the p53 DNA binding motif
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046757
Plasmid#145657PurposeBacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pALS099 (pGEX-2TP-cTevHis-DEST)
Plasmid#139787PurposeGateway destination vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHisDepositorTypeEmpty backboneUseTags6xHis (cleavable by TEV protease) and GST (cleava…ExpressionBacterialMutationPromoterAvailable sinceApril 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sth1Cas9n cytosine base editor
Plasmid#194209PurposeSth1Cas9-D9A-nickase_rAPOBEC1_cytosine base editor for RNA recordingDepositorInsertSth1Cas9-D9A nickase
UseCRISPRTagsExpressionBacterialMutationChanged Aspartate 9 to Alanine.PromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJB042 (FtsZ-mEos2)
Plasmid#49764PurposeInducible expression of FtsZ-mEos2 in bacteriaDepositorInsertFtsZ-mEos2
UseTagsmEos2ExpressionBacterialMutationPromoterT5-lacAvailable sinceMarch 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAC-85b
Plasmid#53282PurposeContains crtE, crtI, crtY genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and will produce beta-carotene in E. coli when complemented with a gene encoding phytoene synthaseDepositorInsertcrtE, crtY, crtI
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAJT430
Plasmid#202291PurposeRPU integration plasmid (LPattB7)DepositorInsertJ23101-RiboJ00-B0034-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only