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Plasmid#145164PurposeMammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity variant 1DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsFc region of human IgG1ExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
NESRA-DEVD-BNLS
Plasmid#50849PurposeExpresses a tandem red ddFP heterodimer in mammalian cells, construct 1 for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid GANLS.DepositorInsertddRFP A and ddRFP B
UseTagsNES sequence LQKKLEELELDE placed after ddGFP A an…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-LiMETER-v1
Plasmid#113933PurposeExpress an optogenetic tether to label and interrogate ER-plasma membrane contact sites (MCS); visualized by GFPDepositorInsertLOV2 and Rit polybasic domain (PB)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTBL680 CHYRON3 integration construct
Plasmid#126448PurposeTo integrate the CHYRON3 locus at AAVS1 in human cells.DepositorInsertspU6/3xLacO-CHYRON3 hgRNA
pCMV-puro
UseTagsExpressionMammalianMutationThe SpCas9 sgRNA constant region is mutated to ma…PromoterCMV and human U6/3xLacOAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Ikkb-FLAG(K44A)
Plasmid#23299DepositorInsertIkkbeta (IKBKB Human)
UseTagsFlagExpressionMammalianMutationK44APromoterAvailable sinceApril 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v2.4(732)-IgG1
Plasmid#154106PurposeMammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (IgG1-Fc fusion)DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsFc region of human IgG1ExpressionMammalianMutationExtracellular, soluble protease and neck domains …PromoterCMVAvailable sinceJune 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v2-Fc(IgG1)
Plasmid#145165PurposeMammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 2DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsFc region of human IgG1ExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v2.4(732)-8h
Plasmid#154103PurposeMammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (8his tag)DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsShort GSG linker and 8his tagExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-G…PromoterCMVAvailable sinceJune 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
CRY2high-mCherry-Raf1
Plasmid#104064PurposeExpresses fusion of CRY2high mutant (CRY2PHR E490R) with mCherry and Raf1DepositorUseTagsExpressionMammalianMutationCRY2PHR E490RPromoterCMVAvailable sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-mTET2 CD mutant
Plasmid#89896PurposeExpresses mouse TET2 CD mutant in mammalian cellsDepositorInsertTET2 (Tet2 Mouse)
UseTagsFlagExpressionMammalianMutationCD (cysteine-rich, dioxygenase domain) truncation…PromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-mTET2 (CD)
Plasmid#89736PurposeExpresses the CD-domain truncation to mouse TET2 in mammalian cellsDepositorInsertTET2 (Tet2 Mouse)
UseTagsFlagExpressionMammalianMutationCD (cysteine-rich, dioxygenase domain) truncation…PromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v2.4-sfGFP
Plasmid#149666PurposeMammalian expression plasmid for sACE2-sfGFP high affinity variant 2.4DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsLinker GSGGSGSGG and superfolder GFPExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 AURKA Lys162Met
Plasmid#157751PurposeExpression of kinase-dead AURKA K62M in mammalian cellsDepositorInsertAURKA (AURKA Human)
UseTagsExpressionMammalianMutationAURKA K162M is a kinase-dead version of AURKAPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-T131D
Plasmid#213600PurposeExpresses human SKP1 T131D mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-T131D (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
aL-EGFP
Plasmid#103879Purposefull-length human aL with EGFP at betapropellerDepositorInsertfull-length human aL with EGFP in betapropeller (ITGAL Human)
UseTagstension sensor moduleExpressionMutationPromoterAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-proα1(I)
Plasmid#119842PurposeExpresses mouse Type I procollagen α1 chain (Col1a1) with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α1 chain (Col1a1 Mouse)
UseTagsmCeruleanExpressionMammalianMutationCol1a1 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-PRMT6
Plasmid#164697PurposeExpression of PRMT6 with an N-terminal FLAG tagDepositorInsertPRMT6 (PRMT6 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTE4497
Plasmid#80339Purposemammalian expression FnCpf1 nuclease and Fn crRNADepositorInsertsFn crRNA
FnCpf1
UseCRISPRTags3xHA and NLSExpressionMammalianMutationPromoterCMV and human U6Available sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Ikka-HA (K44M)
Plasmid#23297DepositorInsertIKK alpha (Chuk Mouse)
UseTagsHAExpressionMammalianMutationK44MPromoterAvailable sinceApril 12, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GFP-IMP2-2_antisense-control
Plasmid#42174DepositorInsertInsulin-like growth factor 2 mRNA binding protein 2-2 antisense control (IGF2BP2 Human)
UseTagsGFPExpressionMammalianMutationPromoterT7Available sinceJune 14, 2013AvailabilityAcademic Institutions and Nonprofits only