Addgene

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pDHt/sk-PC

Plasmid

#92125

Purpose

Expression of codon-optimized Cas9 with Ppdc promoter in Trichoderma reesei

Depositor

Zhihua Zhou

Article

Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1

Insert

Cas9

Use

CRISPR; Trichoderma reesei expression

Tags

Expression

Mutation

Promoter

Ppdc

Availability

Academic Institutions and Nonprofits only

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pDHt/sk-PE

Plasmid

#92126

Purpose

Expression of codon-optimized Cas9-eGFP fusion protein with Ppdc promoter in Trichoderma reesei

Depositor

Zhihua Zhou

Article

Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1

Insert

Cas9-eGFP

Use

CRISPR; Trichoderma reesei expression

Tags

EGFP

Expression

Mutation

Promoter

Ppdc

Availability

Academic Institutions and Nonprofits only

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pDHt/sk-CC

Plasmid

#92127

Purpose

Expression of codon-optimized Cas9 with Pcbh1 promoter in Trichoderma reesei

Depositor

Zhihua Zhou

Article

Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1

Insert

Cas9

Use

CRISPR; Trichoderma reesei expression

Tags

Expression

Mutation

Promoter

Pcbh1

Availability

Academic Institutions and Nonprofits only

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pDHt/sk-CE

Plasmid

#92128

Purpose

Expression of codon-optimized Cas9-eGFP fusion protein with Pcbh1 promoter in Trichoderma reesei

Depositor

Zhihua Zhou

Article

Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1

Insert

Cas9-eGFP

Use

CRISPR; Trichoderma reesei expression

Tags

EGFP

Expression

Mutation

Promoter

Pcbh2

Availability

Academic Institutions and Nonprofits only

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lentiGuide-RRBP1-1

Plasmid

#92156

Purpose

CRISPR guide RNA targeting human RRBP1

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

RRBP1 sgRNA-1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Availability

Academic Institutions and Nonprofits only

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lentiGuide-RRBP1-2

Plasmid

#92157

Purpose

CRISPR guide RNA targeting human RRBP1

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

RRBP1 sgRNA-2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Availability

Academic Institutions and Nonprofits only

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lentiGuide-SYNJ2BP-1

Plasmid

#92159

Purpose

CRISPR guide RNA targeting human SYNJ2BP

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

SYNJ2BP sgRNA-1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Availability

Academic Institutions and Nonprofits only

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lentiGuide-SYNJ2BP-2

Plasmid

#92160

Purpose

CRISPR guide RNA targeting human SYNJ2BP

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

SYNJ2BP sgRNA-2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Availability

Academic Institutions and Nonprofits only

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CMV-sgE1TSS-short-3'box

Plasmid

#92165

Purpose

sgRNA expression vector for Evx1as RNA tethering assay

Depositor

Xiaohua Shen

Article

Luo et al Cell Stem Cell. 2016 May 5;18(5):637-52.

Insert

Evx1as short isoform

Use

CRISPR

Tags

Expression

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pLenti_dCas9-2xAM

Plasmid

#92220

Purpose

Lentiviral plasmid expressing dCas9-2xAM and gRNA cloned in Bbs I sites

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

Inserts

Sp-dCas9-2xAM tag
gRNA to be inserted into Bbs I sites

Use

CRISPR and Lentiviral

Tags

2xAM tag

Expression

Mammalian

Mutation

human codon-optimized, D10A + H840A

Promoter

CBh and U6

Availability

Academic Institutions and Nonprofits only

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pLenti_dCas9-2xAM_hIRF-1

Plasmid

#92221

Purpose

Lentiviral plasmid expressing dCas9-2xAM and gRNA for human IRF-1 promoter

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

Inserts

Sp-dCas9-2xAM tag
gRNA targeting human IRF-1 promoter

Use

CRISPR and Lentiviral

Tags

2xAM tag

Expression

Mammalian

Mutation

human codon-optimized, D10A + H840A

Promoter

CBh and U6

Availability

Academic Institutions and Nonprofits only

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VE-cad-eGFP Donor plasmid

Plasmid

#92309

Purpose

Knock eGFP into human VE-cad locus

Depositor

Sean Palecek

Article

Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

Insert

VEcad Homologous Recombination Arms (CDH5 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

about 30 a.a. truncated at end of protein

Promoter

Availability

Academic Institutions and Nonprofits only

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VE-cad KI gRNA1

Plasmid

#92310

Purpose

CRISPR-GFP-gRNA for cutting VEcad

Depositor

Sean Palecek

Article

Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

Insert

VEcad KI gRNA1 (CDH5 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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VE-cad KI gRNA2

Plasmid

#92311

Purpose

CRISPR-GFP-gRNA for cutting VEcad

Depositor

Sean Palecek

Article

Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

Insert

VEcad KI gRNA2 (CDH5 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pHdzCas9-KRAB

Plasmid

#92341

Purpose

Expression dzCas9-KRAB in microalgae. CRISPR/Cas based plant genome editing and gene regulation, Hyg resistance

Depositor

I-Son Ng

Article

Kao et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Use

CRISPR; Plant expression

Tags

KRAB

Expression

Plant

Mutation

Promoter

CaMV35S

Availability

Academic Institutions and Nonprofits only

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pIKU801-CrPEPC1

Plasmid

#92342

Purpose

Expresses sgRNA for targeting CrPEPC1 gene, paromomycin resistance

Depositor

I-Son Ng

Article

Kao et al Bioresour Technol. 2017 May 4. pii: S096

Insert

sgRNA-CrPEPC1

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

AtU6-26

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-wtSpCas9 (without sgRNA)

Plasmid

#92353

Purpose

Expression plasmid for human codon-optimized wild-type SpCas9 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

3xFlag-NLS-wild-type SpCas9-NLS

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

Promoter

Cbh

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-eSpCas9 (without sgRNA)

Plasmid

#92354

Purpose

Expression plasmid for human codon-optimized high-fidelity eSpCas9 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

3xFLAG-NLS-Streptococcus pyogenes enhanced Cas9-NLS

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

K848A, K1003A, R1060A

Promoter

Cbh

Availability

Academic Institutions and Nonprofits only

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HeFSpCas9

Plasmid

#92355

Purpose

Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity Cas9-NLS

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

Promoter

Cbh

Availability

Academic Institutions and Nonprofits only

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pCAG MCP-VP64

Plasmid

#92357

Purpose

CAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

MCP-VP64

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

Enlarge