Addgene

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pEnt L1L3 EF1a-tTA-3

Plasmid

#24415

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Type

Empty backbone

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2010

Availability

Academic Institutions and Nonprofits only

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pGGE000

Plasmid

#48860

Purpose

Empty entry vector for creating GreenGate terminator modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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R777-E086 Hs.FGFR2-nostop

Plasmid

#70370

Purpose

Gateway ORF clone of human FGFR2 [NM_000141.4] without stop codon (for C-terminal fusions)

Depositor

Dominic Esposito

Insert

FGFR2 (FGFR2 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pSB1A3_GFP_dT34

Plasmid

#89669

Purpose

expression of doubleTAL staple protein dT34 with N-terminally fused cycle3-GFP

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

GFP_dT34

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pSB1A3_GFP_dT23

Plasmid

#89663

Purpose

expression of doubleTAL staple protein dT23 with N-terminally fused cycle3-GFP

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

GFP_dT23

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pME-NLS-tdMCP

Plasmid

#86245

Purpose

Middle entry vector for inserting promotors for tissue specific expression of NLS-tdMCP

Depositor

Florence Marlow

Article

Campbell et al Development. 2015 Apr 1;142(7):1368-74.

Type

Empty backbone

Use

Unspecified

Tags

NLStdMCP

Expression

Mutation

Promoter

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pSB1A3_GFP_dT33

Plasmid

#89668

Purpose

expression of doubleTAL staple protein dT33 with N-terminally fused cycle3-GFP

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

GFP_dT33

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pGGF000

Plasmid

#48861

Purpose

Empty entry vector for creating GreenGate plant resistance cassette modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
R777-E272 Hs.RB1-nostop

Plasmid

#70556

Purpose

Gateway ORF clone of human RB1 [NM_000321.2] without stop codon (for C-terminal fusions)

Depositor

Dominic Esposito

Insert

RB1 (RB1 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pEnt L1L3 tTA-2

Plasmid

#24414

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Type

Empty backbone

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
p3EHA

Plasmid

#67708

Purpose

3' entry HA tag

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

HA tag

Use

Tags

HA

Expression

Mutation

Promoter

None

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pME-6MTnlstdTomato2A

Plasmid

#67685

Purpose

Middle entry nuclear localized tdTomato reporter with 6xMT and 2A

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3796-38

Insert

tdTomato

Use

Tags

2A and 6xMyc-NLS

Expression

Mutation

Promoter

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pSB1A3_GFP_dT17

Plasmid

#89660

Purpose

expression of doubleTAL staple protein dT17 with N-terminally fused cycle3-GFP

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

GFP_dT17

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
R777-E132 Hs.MET-nostop

Plasmid

#70416

Purpose

Gateway ORF clone of human MET [NM_000245.2] without stop codon (for C-terminal fusions)

Depositor

Dominic Esposito

Insert

MET (MET Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221 Rrp8

Plasmid

#63775

Purpose

yeast ORF Rrp8 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Rrp8

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pLG371

Plasmid

#128410

Purpose

Cuminic acid-inducible system

Depositor

Christopher Voigt

Article

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Insert

gfpmut2 (NEWENTRY Synthetic)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pspank(C)

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 Spb1

Plasmid

#63777

Purpose

yeast ORF Spb1 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Spb1

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB1A3_GFP_dT21

Plasmid

#89662

Purpose

expression of doubleTAL staple protein dT21 with N-terminally fused cycle3-GFP

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

GFP_dT21

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB1A3_GFP_dT32

Plasmid

#89667

Purpose

expression of doubleTAL staple protein dT32 with N-terminally fused cycle3-GFP

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

GFP_dT32

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB1A3_GFP_dT16

Plasmid

#89659

Purpose

expression of doubleTAL staple protein dT16 with N-terminally fused cycle3-GFP

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

GFP_dT16

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge