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Plasmid#126072PurposeFor CRISPR/Cas9 delivery in maize. Expresses Cas9 with rice ubiquitin promoter, BAR with 35S promoter, and sgRNA/PTG with rice snoRNA U3 promoter (pol III). Can produce 1 or more gRNAs.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoter35S for BAR; Rice UBI for Cas9; Rice snoRNA U3 fo…Available sinceJune 24, 2019AvailabilityAcademic Institutions and Nonprofits only
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pAAV-Ef1a-DIO ChETA-EYFP
Plasmid#26968PurposeAAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by EF1a promoter for optogenetic activationDepositorHas ServiceAAV1, AAV5, and AAV9InserthChR2(E123T/H134R)-EYFP
UseAAVTagsEYFPExpressionMammalianMutationE123T, H134RPromoterEF1alphaAvailable sinceJan. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
plk1 WT GFP-myc
Plasmid#132963PurposeTo study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damageDepositorInsertpolo-like kinase 1 (PLK1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
sgTrack-mCherry
Plasmid#114013PurposeEmpty sgRNA expression vector with co-expression of mCherry reporterDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hNoxo1
Plasmid#58530Purposeexpresses human Noxo1 in mammalian cellsDepositorInsertNADPH oxidase organizer 1 (NOXO1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mNox4
Plasmid#69353Purposeexpresses mouse Nox4 in mammalian cellsDepositorInsertmNox4 (Nox4 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-C1C2-TS-EYFP
Plasmid#35520DepositorInsertChR1-ChR2 Chimera
UseLentiviralTagsTrafficking signal and EYFPExpressionMammalianMutationPromoterCaMKIIaAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRR-ERalpha-5Z
Plasmid#23061DepositorInsertERalpha (ESR1 Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 31, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLenti-mCherry-CAAX
Plasmid#166229PurposeLentiviral plasmid for expression of mCherry with a C-terminal CAAX polybasic sequence from KRras targeting mCherry to the plasma membrane.DepositorInsertmCherry-CAAX
UseLentiviralTagsCAAXExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/hChR2(H134R)-EYFP
Plasmid#20940PurposeMammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activationDepositorInsertchannelrhodopsin-2
UseTagsExpressionMammalianMutationH134R mutation in ChR2PromoterCMVAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
sgTrack-GFP
Plasmid#114012PurposeEmpty sgRNA expression vector with co-expression of TurboGFP reporterDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-RosaL6 ELD mutations
Plasmid#37198DepositorInsertFokI (fused to Rosa L6 ZFN)
UseZfn expressionTagsHA, ROSA26 eZFNs L6, and SV40 NLSExpressionMammalianMutationQ486E, I499L and N496DPromoterCMVAvailable sinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFLEX+mCyclin D1
Plasmid#86793Purposemammalian expression of cyclin D1DepositorInsertcyclin D1 (Ccnd1 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
Luc-SIRT1 3'UTR
Plasmid#20379DepositorInsertSIRT1 (SIRT1 Human)
UseLuciferaseTagsExpressionMutation3'UTR of SIRT1PromoterAvailable sinceMay 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pT2ADWpuro_2paRAF
Plasmid#129653PurposeFor generating cell line expressing the 2P-activatable RAF system with P2A and full-length RAF1DepositorInsert2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTXRE5-Z
Plasmid#112509PurposeReporter plasmid, responsive to activated Ah receptor/ArntDepositorInsertRW95-3 with 5x xenobiotic response elements (lacZ E.coli, Synthetic)
UseTagsExpressionYeastMutationPromoter5 XREs+ minimal Cytochrome CAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
sgRNA1_Tet-inducible Luciferase reporter
Plasmid#64161PurposePhotoactivatable transcription system. Mammalian expression of sgRNA1 to target Tet-inducible-luciferase reporter.DepositorInsertsgRNA1 for Tet-inducible Luciferase reporter
UseCRISPRTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
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PLKO-mcherry-luc-puro-shSCR
Plasmid#29780DepositorInsertshSCR
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPIDCB2
Plasmid#169900PurposeEmpty dox inducible vector with piggyBac, insulators, and a DTS. The constitutive mClover3 is fused to H2B to allow for visualization of the nucleus.DepositorTypeEmpty backboneUseAvian expressionTagsExpressionMammalianMutationPromoterDox inducibleAvailable sinceDec. 24, 2021AvailabilityAcademic Institutions and Nonprofits only