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Plasmid#87317PurposeExpresses the "Bottom" transcript of the Split-Broccoli system downstream of an RNA Toehold switch Trigger sequence.DepositorInsertSplit-Broccoli "Bottom" transcript downstream of RNA Trigger (TS2_AT01)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterP70aAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
POT5-CYC1p-ycrtYB-ADH1t
Plasmid#65360PurposeTU for combinatorial construction of ?-carotene pathwayDepositorInsertCYC1p-ycrtYB-ADH1t
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJB166
Plasmid#86998PurposeCRISPR/Cas9 in fission yeast using fluoride selectionDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET3a-AqAdk_Y52C
Plasmid#18090DepositorInsertadenylate kinase (kad A. aeolicus)
UseTagsExpressionBacterialMutationTyr 52 changed to CysPromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRS313-PRP8
Plasmid#89966Purposecomplements prp8 genomic deletionDepositorInsertPRP8 (PRP8 Budding Yeast)
UseTagsExpressionYeastMutationnonePromoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-CytERM-N-17
Plasmid#55476PurposeLocalization: Endoplasmic Reticulum, Excitation: 462, Emission: 492DepositorInsertCytERM
UseTagsmTFP1ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-MGC20398
Plasmid#34825DepositorInsertMGC20398 (ZNF830 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTC151
Plasmid#70015PurposeTALENs targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a non-replicating, mutant version of the Gemini Viral RepliconDepositorInsertNuclease (TALENs) + Donor + GVR
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pCas61
Plasmid#82397PurposeIPTG inducible, cLac143 (Markley 2015), LDH V39R with Gmr in glpKDepositorInsertLDH
UseTagsExpressionMutationV39RPromotercLac143Available sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-Laforin Myc R171H
Plasmid#84671Purposemammalian expression and/or retrovirus preparationDepositorArticleInsertLaforin (EPM2A Human)
UseRetroviralTagsMycExpressionMammalianMutationPromoter5'LTRAvailable sinceNov. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
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T7-AglC-His6
Plasmid#89726PurposeExpresses AglC from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.DepositorInsertAglC
UseTagsHis6 and T7ExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
T7-AglK-His
Plasmid#89714PurposeExpresses AglK from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.DepositorInsertAglK
UseTagsHis6 and T7ExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
His8-TEV-PseC
Plasmid#89724PurposeExpresses PseC from C. jejuni with an N-terminal octaHis tag and TEV cleavage siteDepositorInsertPseC (pseC Campylobacter jejuni)
UseTagsHis8 and TEVExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-PglF130
Plasmid#89708PurposeExpresses truncated PglF from C. jejuni with N-terminal GST tagDepositorInsertpglF
UseTagsGSTExpressionBacterialMutationresidues 1-129 of putative transmembrane domain h…PromoterAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7ddx19gRNA
Plasmid#47932Purposein vitro transcription of ddx19 gRNADepositorInsertddx19 gRNA (ddx19 Zebrafish)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
MSCV-PM-BAF180-SH2
Plasmid#41932DepositorInsertBAF180
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRS314-PRP16
Plasmid#89971PurposePrp16 in a trp backbone complements prp16 genomic deletionDepositorInsertPRP16 (PRP16 Budding Yeast)
UseTagsExpressionYeastMutationnonePromoterAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only