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Plasmid#222444PurposeLevel 0, N-terminal tag module (CCAT-AATG) containing LoxP-mCherry-stop.DepositorInsertLoxP-mCherry-stop
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW110034
Plasmid#222443PurposeLevel 0, N-terminal tag module (CCAT-AATG) containing LoxP-mCherry.DepositorInsertLoxP-mCherry
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
PtetA with pBELO_L6-PT3-T4_CAMR_TAN+
Plasmid#225644PurposetetA promoter expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistanceDepositorInserttetR/tetA
UseTagsmCherryExpressionBacterialMutationPromotertetA promoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
PLac0301 with pBELO_L6-PT3-T4_CAMR_TAN+
Plasmid#225643PurposeLac0301 promoter expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistanceDepositorInsertLacO3O1
UseTagsmCherryExpressionBacterialMutationPromoterLacO3O1 promoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
ParaBAD with pBELO_L6-PT3-T4_CAMR_TAN+
Plasmid#225642PurposearaBAD promoter expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistanceDepositorInsertaraBAD
UseTagsmCherryExpressionBacterialMutationPromoterBAD promoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1177
Plasmid#217961PurposeDual cistronic expression vector for the RAPID-release approach. H3.2-mCherry is released from mitochondria to the nucleus upon addition of rapamycin.DepositorInsertHistone H3 (HIST2H3PS2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB5230
Plasmid#215496PurposeA pAde6 backbone integrating plasmid expressing Pil1-mCherry-SpAtg8(1-115) under the inducible promoter 41nmt1DepositorInsertPil1-mCherry-SpAtg8(1-115)
UseTagsExpressionYeastMutationPromoterP41nmt1Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCX:γB2ΔEC1ΔICD-mCh
Plasmid#208521PurposemCherry-fused PcdhγB2 lacking an EC1 domain and an ICD domainDepositorInsertPcdhgB2 (Pcdhgb2 Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterChicken β-actinAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB3236
Plasmid#190401PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proTT*::GFPint_ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_proTT*::GFPint_ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
Receiver_plasmid1
Plasmid#140694PurposeEncodes constitutive red (mCherry) fluorescence for identificationDepositorInsertmCherry
UseTagsssrA degradation tag (ASV)ExpressionBacterialMutationPromoterpIq constitutive promoterAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFGL1259R
Plasmid#118995PurposemCherry (without start and stop codons) in pGFL1010DepositorInsertmCherry (without start and stop codons)
UseFungal expressionTagsExpressionMutationPromoterpromoter lessAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVH011
Plasmid#80395PurposeContains bicistronic pTet-Scaffold(3xFLAG)- ShineDelgarno-mCherry and TetR genes. Used for Western blots.DepositorInsertBicistronic Scaffold-3xFLAG-SD-mCherry
UseSynthetic BiologyTags3xFLAG tag on scaffold protein for WesternExpressionBacterialMutationPromoterAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMB-TGNVHAr
Plasmid#61179PurposeFluorescent reporter for trans-Golgi network AtVHA-a1-mCherry expressed under MtBCP1 promoterDepositorInsertAtVHA-a1
UseTagsmCherryExpressionPlantMutationPromoterMtBCP1Available sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMB-GAr
Plasmid#61173PurposeFluorescent reporter for Golgi apparatus GmMAN49-mCherry expressed under MtBCP1 promoterDepositorInsertGmMAN49-mCherry
UseTagsExpressionPlantMutationPromoterMtBCP1Available sinceApril 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMB-TGN41r
Plasmid#61177PurposeFluorescent reporter for trans-Golgi network mCherry-MtSYP41 expressed under MtBCP1 promoterDepositorInsertMtSYP41
UseTagsmCherryExpressionPlantMutationPromoterMtBCP1Available sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMB-LEr
Plasmid#61186PurposeFluorescent reporter for late endosome mCherry-MtRAB5A2 expressed under MtBCP1 promoterDepositorInsertMtRAB5A2
UseTagsmCherryExpressionPlantMutationPromoterMtBCP1Available sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMB-ERr
Plasmid#61171PurposeFluorescent reporter for endoplasmic reticulum AtWAK2sp-mCherry-HDEL expressed under MtBCP1 promoterDepositorInsertAtWAK2-mCherry-HDEL
UseTagsExpressionPlantMutationPromoterMtBCP1Available sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II donor only control (F40-based tension sensor)
Plasmid#118717PurposeThe donor (YPet(short)) only control for the F40-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET.DepositorInserthuman Desmoplakin II-[YPet(short)-F40-mCherry(Y72L)] (internal-1353) (DSP Human)
UseRetroviralTagsYPet(short)-F40-mCherry(Y72L)ExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterCMVAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II donor only control (F40-based no force control)
Plasmid#118718PurposeThe donor (YPet(short)) only control for the no force control of the F40-based human desmoplakin II tension sensor provides the donor only lifetime used in lifetime measurements to determine FRET.DepositorInserthuman Desmoplakin II (1-1353)-[YPet(short)-F40-mCherry(Y72L)] (DSP Human)
UseRetroviralTagsYPet(short)-F40-mCherry(Y72L)ExpressionMammalianMutationtruncation after aa1353; Y72L mutation in mCherry…PromoterCMVAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II donor only control (FL-based tension sensor)
Plasmid#118723PurposeThe donor only (YPet(short)) control for the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET.DepositorInserthuman Desmoplakin II-[YPet(short)-FL-mCherry(Y72L)] (internal-1353) (DSP Human)
UseRetroviralTagsYPet(short)-FL-mCherry(Y72L)ExpressionMammalianMutationinserted FL-based tension sensor module after aa1…PromoterCMVAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only