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Plasmid#118061PurposeGBPart - Bovine growth hormone terminatorDepositorInsertbgH_poly(A)
UseSynthetic Biology; GbpartTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB103
Plasmid#68557PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB102
Plasmid#68556PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Blast DEST (w524-1)
Plasmid#19065Purpose3rd gen lentiviral Gateway destination vector, expression, PGK promoter, BlastDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMRE141
Plasmid#118493Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE143
Plasmid#118495Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertsYFP2
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
MAC-tag-N
Plasmid#108078PurposeGateway compatible destination vectors containing StrepIII/HA/BioIDDepositorInsertCCDB (ccdB(letD) )
UseTagsStrepIII-HA-BirA*ExpressionBacterial and MammalianMutationPromoterAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-TET
Plasmid#20909PurposepiggyBac empty vector with Gateway cassette, tet inducible promoter, and linked reporter (IRES-bgeo)DepositorInsertGateway Destination Vector
UsepiggybacTagsIRES-bGeoExpressionMammalianMutationPromoterAvailable sinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
plenti-UBC-HA-gate-pGK-HYG
Plasmid#107396PurposeLentiviral gateway destination vectorDepositorTypeEmpty backboneUseLentiviral; Gateway destinationTagsHAExpressionMammalianMutationPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLG021 (AVAST type 5)
Plasmid#157899PurposeExpresses the AVAST type 5 defense systemDepositorInsertAVAST type 5 (STAND ATPase with N-terminal SIR2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Neo DEST (w531-1)
Plasmid#19067Purpose3rd gen lentiviral Gateway destination vector, expression, PGK promoter, NeoDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing the MBP sybody Sb_MBP#1
Plasmid#132699Purposeexpresses the MBP sybody Sb_MBP#1 in E.coli, to use as positive control in ELISADepositorInsertsybody_MBP#1
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-TAG-ERP2
Plasmid#80479PurposeAll-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)DepositorTypeEmpty backboneUsePiggybac transposonTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)::NL
Plasmid#141289PurposeNLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertNLUC
UseLuciferaseTagsExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbA5c-MevT(CO)-MBIS(CO, ispA)
Plasmid#35151DepositorInsertAtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA
UseSynthetic BiologyTagsExpressionBacterialMutationR364S in Mevalonate KinasePromoterLacUV5,Available sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLR16-Phes*
Plasmid#98783PurposeSuicide vector for allelic exchange in Listeria utilizing an engineered counterselection marker for Listeria monocytogenes based on a mutated phenylalanyl-tRNA synthetase gene (pheS*).DepositorInsertpheS
UseTagsExpressionBacterialMutationA309GPromoterAvailable sinceJan. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL0915 (CRISPR with target 3)
Plasmid#130643PurposeExpresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA that targets lacZ.DepositorInsertCRISPR(target-3)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterT7Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCBC-MT2T3
Plasmid#50594PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT2, gRNA scaffold, TaU3t plus, U6-26p, T3
UseCRISPR; Pcr templateTagsExpressionPlantMutationPromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_WTDHFR_WTTS
Plasmid#91226Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-Flag-HA
Plasmid#101766PurposeGateway-compatible destination vector for mammalian expression of N-terminal HA & Flag-tag fusion proteinDepositorTypeEmpty backboneUseTagsFlag-HAExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only