Addgene

Showing: 4441 - 4460 of 30516 results

Results per page:

Sort results by:

CMVTO-nTVMVP-AP4-tevs-P3-tevs-cTVMVPmut

Plasmid

#179660

Purpose

Cytosolic TEVP-inducible caged-nTVMVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Caged-nTVMVP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CMVTO-nTVMVPmut-tevs-AP4-tevs-P3-cTVMVP

Plasmid

#179661

Purpose

Cytosolic TEVP-inducible caged-cTVMVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Caged-cTVMVP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-CMV-Rheb(D60I)-IRES-eGFP-W

Plasmid

#32521

Depositor

Mustafa Sahin

Insert

Dominant negative Rheb (D60I) (Rheb Rat)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

D60I to make dominant negative

Promoter

CMV

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
p6900 PHAGE-P CMVT N-HA-hE6AP III C840A

Plasmid

#37606

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP III C840A (UBE3A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C840A

Promoter

CMV tet regulatable

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-ABEmax(TadA E59A, TadA*E59A)

Plasmid

#125649

Purpose

pCMV-ABEmax(TadA E59A, TadA*E59A) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A TadA* E59A)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-CMV-cGAS R71/75E C396/7A-HA

Plasmid

#130920

Purpose

Expresses human cGAS with R71/75E and C396/397A point mutations; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

human cGAS R71/75E C396/7A

Use

Tags

HA

Expression

Mammalian

Mutation

aa 71 and 75 mutated to glutamate; aa 396 and 397…

Promoter

CMV

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-Tagged Clavesin1 (pCMV-Tag2B-Sec14a)

Plasmid

#47430

Depositor

Peter McPherson

Article

Katoh et al J Biol Chem. 2009 Oct 2;284(40):27646-54

Insert

Clavesin1 (CLVS1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
Flag-Tagged Clavesin2 (pCMV-Tag2B-Sec14b)

Plasmid

#47431

Depositor

Peter McPherson

Article

Katoh et al J Biol Chem. 2009 Oct 2;284(40):27646-54

Insert

Clavesin2 (CLVS2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-Myc-XPLN aa1-125 (N125)

Plasmid

#73378

Purpose

Expression of the N-terminal region of XPLN (amino acids 1–125, designated N125)

Depositor

Jie Chen

Article

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Use

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

Expresses N terminal amino acid 1-125 of XPLN (ar…

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pCMV10/3xflag-Clock L57E/L113E/W284A

Plasmid

#47343

Purpose

triple mutation used for co-IP

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Use

Tags

3x Flag

Expression

Mammalian

Mutation

L57E/L113E/W284A

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pCMV5 MYC-Smurf2 (FF29/30A, Y459A) WT

Plasmid

#24606

Depositor

Jeff Wrana

Article

Ogunjimi et al J Biol Chem. 2010 Feb 26. 285(9):6308-15

Insert

Smurf2 (SMURF2 Human)

Use

Tags

MYC

Expression

Mammalian

Mutation

F29A, F30A, Y459A

Promoter

Available since

May 5, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pCMV5-Flag-EPLIN beta delta C term

Plasmid

#40931

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN beta (LIMA1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

C terminus deleted

Promoter

Available since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
AAV-CMV-NLS-mTurquoise2-optoNES-WPRE

Plasmid

#159962

Purpose

optical regulation of nuclear export

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2-optoNES

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

synthetic

Promoter

CMV-IE

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHDR-CDKN2A-Ex2-CMV-H2B-mOrange2

Plasmid

#110735

Purpose

Recombination template for replacing CDKN2A Exon2 with Ef1alpha-H2B-mOrange2

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

H2B-mOrange2

Use

Other

Tags

Expression

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CMV-dSa-VPR mini.-1X snRP1

Plasmid

#99687

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 17 nt snRP1 poly adenylation signal

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VPR mini

Expression

Mutation

dead Cas9

Promoter

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-ADA add back mutant pCMV5

Plasmid

#17554

Depositor

Domenico Accili

Article

Kitamura et al J Clin Invest. 2007 Sep . 117(9):2477-85

Insert

FLAG-FoxO1-ADA

Use

Tags

FLAG

Expression

Mammalian

Mutation

siRNA-resistant Foxo1 by replacing 3 residues (un…

Promoter

Available since

Oct. 17, 2008

Availability

Academic Institutions and Nonprofits only
pDEST-CMV 3xFLAG-LC3B-G120A-GFP

Plasmid

#123108

Purpose

Expresses 3xFLAG-LC3B-G120A-EGFP in mammalian cells, negative control for monitoring ATG4 activity.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Tags

3xFLAG and EGFP

Expression

Mammalian

Mutation

Glycine 120 to Alanine

Promoter

CMV

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-ABEmax(TadA E59A, TadA*V106Q)

Plasmid

#125651

Purpose

pCMV-ABEmax(TadA E59A, TadA*V106Q) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A TadA* V106Q)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-ABEmax(TadA E59A, TadA*R47Q)

Plasmid

#125657

Purpose

pCMV-ABEmax(TadA E59A, TadA*R47Q) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A TadA* R47Q)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-ABEmax(TadA E59A, TadA*D108Q)

Plasmid

#125655

Purpose

pCMV-ABEmax(TadA E59A, TadA*D108Q) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A TadA* D108Q)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge