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Plasmid#72982PurposeN-terminal linker with pET-RBS (5' GTAC/3' TAAA fusion)DepositorInsertN-terminal linker with pET-RBS
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-HexHis
Plasmid#72983PurposeHexahistidine N-terminal tag (5' TAAA/3' CATA fusion)DepositorInsertPolyhistidine (HHHHHH) N-terminal tag
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-ORF-eCFP
Plasmid#72961PurposeLevel 0 Entry vector" ORF - eCFP"DepositorInsertEnhanced cyan fluorescent protein (eCFP)
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-ORF-mCherry
Plasmid#72962PurposeLevel 0 Entry vector" ORF - mCherry "DepositorInsertmCherry fluorescent protein
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-ORF-eGFP
Plasmid#72960PurposeLevel 0 Entry vector" ORF - eGFP "DepositorInsertEnhanced green flourescent protein (eGFP)
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
AEB-T4-2
Plasmid#63855PurposeThis pFTV1 vector contains a thyroxine binding riboswitch, controlling the translation initaition rate of luciferase gene.DepositorInsertT4-2 thyroxine riboswitch
UseSynthetic BiologyTagsExpressionMutationPromotertacAvailable sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSTARR-seq_fly-x16
Plasmid#71505PurposeflySTARR-seq screening vector with x16 core promoterDepositorTypeEmpty backboneUseFlystarr-seq screening vectorTagssgGFP (SuperGlo, Qbiogene, Inc)ExpressionInsectMutationPromoterAvailable sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJB051 (mEos2-ZapA)
Plasmid#72650PurposeInducible expression of mEos2-ZapA in bacteriaDepositorInsertmEos2-ZapA
UseTagsmEos2ExpressionBacterialMutationPromoterT5-lacAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3-RpS12-Gateway
Plasmid#71508PurposeflySTARR-seq validation vector with Gateway cassette downstream of the firefly luciferase gene and the RpS12 core promoterDepositorTypeEmpty backboneUseFlystarr-seq validation vectorTagsluciferaseExpressionInsectMutationPromoterAvailable sinceDec. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSTARR-seq_fly-eEF1delta
Plasmid#71501PurposeflySTARR-seq screening vector with eEF1delta core promoterDepositorTypeEmpty backboneUseFlystarr-seq screening vectorTagssgGFP (SuperGlo, Qbiogene, Inc)ExpressionInsectMutationPromoterAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJBL-RNAINS4
Plasmid#69456PurposeRNA-IN S4 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)DepositorInsertRNA-IN S4 + SFGFP
UseSynthetic BiologyTagsExpressionBacterialMutationmutations for S4 variant (from wt)PromoterJ23119 and J23119 (sigma 70)Available sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJBL-RNAINS3
Plasmid#69455PurposeRNA-IN S3 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)DepositorInsertRNA-IN S3
UseSynthetic BiologyTagsExpressionBacterialMutationmutations for S3 variant (from wt)PromoterJ23119 and J23119 (sigma 70)Available sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA5woH
Plasmid#65019PurposeExpresses the P94R/D160N/D165K/K190E/K196T mutant of Staphylococcus aureusDepositorInsertsortase A
UseTagsS tagExpressionBacterialMutationdeleted amino acids 1-59, P94R, D160N, D165A, K19…Promotertetracycline promoter/operatorAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA2woH
Plasmid#65018PurposeExpresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsS tagExpressionBacterialMutationdeleted amino acids 1-59, E105K, E108APromotertetracycline promoter/operatorAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSALECT-SupH
Plasmid#59710PurposepSALECT with SupH insertDepositorInsertSupH
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSALECT-AhyI
Plasmid#59708PurposepSALECT with AhyI insertDepositorInsertAhyI
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-D91G/V220A
Plasmid#47648PurposepAC-EsaR with a272g and c659t mutations in esaRDepositorInsertesaR-D91G/V220A
UseSynthetic BiologyTagsExpressionBacterialMutationChanged Aspartic acid 91 to Glycine, and Valine 2…PromoterPlacAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSCT-GAL93-DEST
Plasmid#46339DepositorTypeEmpty backboneUseTags6XHISExpressionMammalianMutationPromoterpCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNH066
Plasmid#42162DepositorInsertN-TAP Tag-RT-::mTFP1
UseTagsTAP-TAgExpressionMutationPromoterAvailable sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNH094
Plasmid#42167DepositorInsertmTFP1-RT-::[(G4S)3]::C-TAP Tag
UseTagsExpressionMutationPromoterAvailable sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only