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pUC57-mini v2-HF24_F21

Plasmid

#195735

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#21 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-TAPtagN-PrP-SBP-HsAGO2_AC

Plasmid

#148440

Purpose

Mammalian Expression of HsAGO2

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsAGO2 (AGO2 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation C840Tand sequence differs in …

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot1iso1-FNKRNFKFEtoAAAAAAAAA-shRNAres_AC

Plasmid

#148442

Purpose

Mammalian Expression of HsNot1iso1-FNKRNFKFEtoAAAAAAAAA-shRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsNot1iso1-FNKRNFKFEtoAAAAAAAAA-shRNAres (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ1373_mU6_sgRNA targeting e1

Plasmid

#190684

Purpose

sgRNA targeting enhancer 1 of MYC

Depositor

Stanley Qi

Article

Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

Insert

sgRNA targeting enhancer 1 of MYC

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

BFP and Puromycin

Expression

Mammalian

Mutation

Promoter

mouse U6 promoter

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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double_sgRNA targeting e1 and e4

Plasmid

#190688

Purpose

sgRNAs targeting enhancer 1 and 4 of MYC separately

Depositor

Stanley Qi

Article

Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

Insert

sgRNAs targeting enhancer 1 and 4 of MYC separately

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

BFP and Puromycin

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ5004_hU6_sgRNA targeting e7

Plasmid

#190687

Purpose

sgRNA targeting enhancer 7 of MYC

Depositor

Stanley Qi

Article

Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

Insert

sgRNA targeting enhancer 7 of MYC

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

BFP and Puromycin

Expression

Mammalian

Mutation

Promoter

Human U6 promoter

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ5004_hU6_sgRNA targeting e4

Plasmid

#190686

Purpose

sgRNA targeting enhancer 4 of MYC

Depositor

Stanley Qi

Article

Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

Insert

sgRNA targeting enhancer 4 of MYC

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

BFP and Puromycin

Expression

Mammalian

Mutation

Promoter

Human U6 promoter

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ1373_mU6_sgRNA targeting e3

Plasmid

#190685

Purpose

sgRNA targeting enhancer 3 of MYC

Depositor

Stanley Qi

Article

Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

Insert

sgRNA targeting enhancer 3 of MYC

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

BFP and Puromycin

Expression

Mammalian

Mutation

Promoter

mouse U6 promoter

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pMX-SHP1C/S-ires-mCD8

Plasmid

#183719

Purpose

Expresses the myc-tagged SHP-1 C453S mutant (SHP-1C/S) (substrate-trapping mutant) and mouse CD8.

Depositor

Takeshi Tsubata

Article

Alborzian Deh Sheikh et al J Immunol. 2021 Jun 1;206(11):2544-2551.

Insert

SHP-1

Use

Retroviral

Tags

myc

Expression

Mutation

C453S

Promoter

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso2-6xmut_U

Plasmid

#147732

Purpose

Mammalian Expression of HsNot1iso2-6xmut

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot1iso2-6xmut (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

4 silent and two non silent G917E and R2353Q muta…

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso2-del1844-2133_T

Plasmid

#147628

Purpose

Mammalian Expression of HsNot1iso2-del1844-2133

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot1iso2-del1844-2133 (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

4 silent and two non silent G917E and R2353Q muta…

Promoter

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsRck_296-472-10xmut_AC

Plasmid

#148420

Purpose

Mammalian Expression of HsRck_296-472-10xmut

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck_296-472-10xmut (DDX6 Human)

Use

Tags

Expression

Mammalian

Mutation

Q309A, H312A, T316A, R320A, Q322A, N324A, R335A, …

Promoter

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot3-del607-634-F673E_S

Plasmid

#147489

Purpose

Mammalian Expression of HsNot3-del607-634-F673E

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot3-del607-634-F673E (CNOT3 Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutations compared to the sequence giv…

Promoter

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDON207 NSP3 K977Q

Plasmid

#180057

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 NSP3 K977Q (ORF1ab SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

NSP3 K977Q

Promoter

Available since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON207 NSP3 A890D

Plasmid

#180069

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 NSP3 A890D (ORF1ab SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

NSP3 A890D

Promoter

Available since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON221 ORF3a Q57H

Plasmid

#180072

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 ORF3a Q57H (ORF3a SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

ORF3a Q57H

Promoter

Available since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 Spike D138Y

Plasmid

#180056

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike D138Y (S SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike D138Y

Promoter

Available since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 N S235F

Plasmid

#180071

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 N S235F (N SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

N S235F

Promoter

Available since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 NSP5 K90R

Plasmid

#180073

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 NSP5 K90R (ORF1ab SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

NSP5 K90R

Promoter

Available since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 Spike A570D

Plasmid

#180064

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike A570D (S SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike A570D

Promoter

Available since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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